Kanamycin activates caspase-1 in NC/Nga mice

Abstract

Abuse of antibiotics to treat children has been associated with an increased risk of the development of inflammatory diseases. The underlying mechanism behind this association still remains to be clarified. Here, we examined the mechanisms behind kanamycin‐induced skin inflammation in NC/Nga mice. NC/Nga mice were orally administered kanamycin for 7 days consecutively. Blood, spleen and dorsal skin were taken 18 weeks after kanamycin treatment was stopped. Kanamycin significantly increased the allergic reaction. We also observed significant increases in caspase‐1 mRNA and protein expression in the dorsal skin of the kanamycin‐administered mice compared to the control mice. The increased enzymatic activity of caspase‐1 in the dorsal skin of the kanamycin‐administered mice increased the mRNA expressions of IL‐1β and IL‐18. The productions of IL‐1β and IL‐18 were also increased in the splenocytes obtained from kanamycin‐administered mice. Kanamycin upregulated the TNF‐α mRNA expression in the dorsal skin and the TNF‐α production in stimulated splenocytes. The activation of nuclear factor‐κB and degradation of IκBα were increased by kanamycin administration. Our findings suggest that the use of kanamycin during infancy may increase the potential for skin inflammatory reactions through the upregulation of caspase‐1.

Inhibition of IL-32 and TSLP production through the attenuation of caspase-1 activation in an animal model of allergic rhinitis by Naju Jjok (Polygonum tinctorium)

“…Caspase-1 levels in allergic asthmatic patients are also higher than those in normal individuals (38). IL-1β, IL-18, histamine and IgE have been shown to be upregulated through the activation of caspase-1 in kanamycin-administered NC/Nga mice (39). IL-32 and TSLP production are also increased by the activation of caspase-1 (7,20).…”

Cysteine induces longitudinal bone growth in mice by upregulating IGF-I

“…. qPCR was performed using SYBR-Green Master Mix, and the detection of mRNA was carried out using an ABI StepOne real-time PCR system (Applied Biosystems, Foster City, CA, USA), as previously described (25)(26)(27). PCR was performed with the following primers: human IGF-I forward, 5'-TGC CCA AGA CCC AGA AGT-3' and reverse, 5'-CTC CTG TCC CCT CCT TCT GTT-3'; human GAPDH forward, 5'-TCG ACA GTC AGC CGC ATC TTC TTT-3' and reverse, 5'-ACC AAA TCC GTT GAC TCC GAC CTT-3'; mouse IGF-I forward, 5'-CCG GAC CAG AGA CCC TTT G-3' and reverse, 5'-CCT GTG GGC TTG TTG AAG TAA AA-3'; and mouse GAPDH forward, 5'-GGC AAA TTC AAC GGC ACA-3' and reverse, 5'-GTT AGT GGG GTC TCG CTC CTG-3'.…”

Taurine, a major amino acid of oyster, enhances linear bone growth in a mouse model of protein malnutrition

“…Total RNA was isolated from liver tissues using an easy‐BLUE™ RNA extraction kit (iNtRON Biotech, Republic of Korea) and quantitative real‐time PCR was performed using a SYBR Green master mix. mRNA was analyzed using an ABI StepOne real‐time PCR System (Applied Biosystems, Foster City, CA), as previously described .…”