Kallikrein-binding protein inhibits retinal neovascularization and decreases vascular leakage

Gao, Guoquan; Shao, Chen; Zhang, S. X.; Dudley, Andrew C.; Fant, James; Ma, Jichi

doi:10.1007/s00125-003-1085-9

Cited by 60 publications

(70 citation statements)

References 48 publications

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“…S2). 5 Western blot also showed that KBP treatment markedly down-regulated VEGF expression in cultured tumor cells and tumor tissues. Densitometric analysis of the bands showed VEGF protein levels in cultured tumor cells treated with KBP were decreased in a dose-dependent manner (Fig.…”

Section: Kbp Down-regulates Vegf In Tumor Cells and Tumor Xenograftsmentioning

confidence: 85%

“…The KBP/ pET28 construct was introduced into Escherichia coli strain BL-21/DE3 (Novagen). The expression and purification were carried out as described previously (5).…”

Section: Cell Culturementioning

confidence: 99%

“…Columns, mean; bars, SE (*P < 0.05, **P < 0.01). 5 Supplementary material for this article is available at Molecular Cancer Therapeutics Online (http://mct.aacrjournals.org/).…”

Section: Kbp Suppresses Growth and Metastasis Of Human Gastric Carcinomamentioning

confidence: 99%

“…Many serpins, such as pigment epithelium-derived factor, antithrombin, and maspin, have been shown to have antiangiogenic activity (10 -12). As a member of serpin superfamily, KBP also shows antiangiogenic property and has been identified as an endogenous angiogenic inhibitor (4,5,13). We previously showed that intravitreal injection of KBP inhibited retinal neovascularization and decreased vascular permeability of retina, iris, and choroid in rats with an oxygen-induced retinopathy (5).…”

Section: Introductionmentioning

confidence: 99%

“…We previously found that KBP inhibited retinal angiogenesis and decreased vascular permeability by reducing vascular endothelial growth factor (VEGF) production in endothelial cells and blocking VEGF binding to endothelial cells (5). However, the effect of KBP on the expression and release of VEGF in tumor cells has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

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Kallikrein-binding protein inhibits growth of gastric carcinoma by reducing vascular endothelial growth factor production and angiogenesis

Zhu¹,

Lü²,

Cai³

et al. 2007

Molecular Cancer Therapeutics

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Kallikrein-binding protein (KBP) has been identified as an endogenous angiogenic inhibitor. We previously showed that KBP inhibited rat retinal neovascularization by downregulation of vascular endothelial growth factor (VEGF) in endothelial cells. However, its antiangiogenic potential for inhibition of gastric carcinoma and the effect on VEGF in tumor cells have not been elucidated. The present study was designed to investigate the effect of KBP on growth of gastric carcinoma and the possible molecular mechanism. Recombinant KBP dose dependently inhibited proliferation and induced apoptosis of endothelial cells, but no effect on proliferation and apoptosis of SGC-7901 gastric carcinoma cells. I.p. injection of KBP resulted in growth inhibition of both heterotopic and orthotopic gastric carcinoma xenografts at 61.4% and 52.3%, respectively. Microvessel density in tumor tissues treated with KBP was significantly decreased, suggesting that KBP suppressed tumor growth by antiangiogenesis. The expression and release of VEGF, a major angiogenic stimulator, were down-regulated by KBP in SGC-7901 cells and gastric carcinoma xenografts. RNA levels of VEGF in SGC-7901 cells were also decreased by KBP, thus suggesting the regulation at the transcriptional level.

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Section: Kbp Down-regulates Vegf In Tumor Cells and Tumor Xenograftsmentioning

confidence: 85%

“…The KBP/ pET28 construct was introduced into Escherichia coli strain BL-21/DE3 (Novagen). The expression and purification were carried out as described previously (5).…”

Section: Cell Culturementioning

confidence: 99%

“…Columns, mean; bars, SE (*P < 0.05, **P < 0.01). 5 Supplementary material for this article is available at Molecular Cancer Therapeutics Online (http://mct.aacrjournals.org/).…”

Section: Kbp Suppresses Growth and Metastasis Of Human Gastric Carcinomamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Kallikrein-binding protein inhibits growth of gastric carcinoma by reducing vascular endothelial growth factor production and angiogenesis

Zhu¹,

Lü²,

Cai³

et al. 2007

Molecular Cancer Therapeutics

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Current literature in diabetes

2003

Diabetes Metabolism Res

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of diabetes/metabolism. Each bibliography is divided into 17 sections: 1 Books, Reviews & Symposia; 2 General; 3 Genetics; 4 Epidemiology; 5 Immunology; 6 Prediction; 7 Prevention; 8 Intervention: a) General; b) Pharmacology; 9 Pathology: a) General; b) Cardiovascular; c) Neurological; d) Renal; 10 Endocrinology & Metabolism; 11 Nutrition; 12 Animal Studies; 13 Techniques. Within each section, articles are listed in alphabetical order with respect to author (10 Weeks journals ‐ Search completed at 17th Sept. 2003)

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Omic analyses unravels global molecular changes in the brain and liver of a rat model for chronic Sake (Japanese alcoholic beverage) intake

et al. 2009

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The effects of chronic administration of Sake (Japanese alcoholic beverage, Nihonshu) on brain and liver of female F334 (Fisher) rats were surveyed via global omic analyses using DNA microarray, 2-DE, and proton nuclear magnetic resonance. Rats weaned at 4 wk of age were given free access to Sake (15% alcohol), instead of water. At 13 months of age, and 24 h after withdrawal of Sake supply, rats were sacrificed, and the whole brain and liver tissues dissected for analyses. In general, molecular changes in brain were found to be less than those in liver. Transcriptomics data revealed 36 and 9, and 80 and 62 up- and down-regulated genes, in the brain and liver, respectively, with binding and catalytic activity gene categories the most prominently changed. Results suggested Sake-induced fragility of brain and liver toxicity/damage, though no significant abnormalities in growth were seen. At protein level, a striking decrease was found in the expression of NADH dehydrogenase (ubiquinone) Fe-S protein 1 in brain, suggesting attenuation of mitochondrial metabolism. In liver, results again suggested an attenuation of mitochondrial function and, in addition, glycoproteins with unknown function were induced at protein and gene levels, suggesting possible changes in glycoprotein binding in that organ. Metabolomic analysis of brain revealed significant increases in valine, arginine/ornithine, alanine, glutamine, and choline with decreases in isoleucine, N-acetyl aspartate, taurine, glutamate, and gamma aminobutyric acid. Our results provide a detailed inventory of molecular components of both brain and liver after Sake intake, and may help to better understand effects of chronic Sake drinking.

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Kallikrein-binding protein inhibits retinal neovascularization and decreases vascular leakage

Cited by 60 publications

References 48 publications

Kallikrein-binding protein inhibits growth of gastric carcinoma by reducing vascular endothelial growth factor production and angiogenesis

Kallikrein-binding protein inhibits growth of gastric carcinoma by reducing vascular endothelial growth factor production and angiogenesis

Current literature in diabetes

Omic analyses unravels global molecular changes in the brain and liver of a rat model for chronic Sake (Japanese alcoholic beverage) intake

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