KAJIAN HISTOLOGI OVARIUM IKAN SIDAT, Anguilla bicolor McClelland, YANG TERTANGKAP DI SEGARA ANAKAN CILACAP

Rachmawati, Farida Nur; Susilo, Untung

doi:10.23869/bphjbr.18.1.20128

Cited by 9 publications

(7 citation statements)

References 6 publications

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“…Both data culture, freshwater and brackish water, significantly improve the eye and fin index (Figure 2.). This study also confirm earlier study that no real correlation between sex (male) and length ( Table 1), except that eel of over ≥ 45 cm in length were females (Colombo et al, 1984;Colombo & Grandi, 1990;Colombo & Grandi, 1995;Tesch, 2003;Rachmawati & Susilo, 2012;Rachmawati et al, 2017;Arai & Kadir, 2017). In nature eel needs marine or brackish water to get maturation stage for reproduction, so we call eel as a catadromous species.…”

Section: Resultssupporting

confidence: 90%

Effectively Of 17α-Methyltestosterone on Tropical Eel, Anguilla bicolor McClelland Masculinization in Different Salinity Culture

2018

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Eel population in nature reaches critical number, so that culture strategy is urgently needed to fulfil the high demand of this fish. A shortcut to get functional male, which proven difficult to be founded from natural catching, is masculinization. This research aimed to induce masculinization of tropical eel, Anguilla bicolor McClelland supplemented with various doses of 17α-methyltestosterone during a month culture in freshwater (0 ppt) or brackish water (10 ppt). The eel was grouped and fed with supplementation of 17α-methyltestosterone at various doses, depending upon treatments, namely 0 mg Kg-1 diet (control), 40, 80 or 120 mg Kg-1 diet. Eels size were similar, at approximately 16,78 g ± 0,62 in weight and 25,38 cm ± 0,15 in length were either culture in freshwater or brackish during the experiment for eight weeks. Sex gonad, based on anatomical histological structures, Eye Index and Fin Index were measured after time culture treatment achieved, as well as body length, weight, eye diameter and the length of the pectoral fin were measured. Results showed that supplementation 17α-methyltestosterone 80 mg/Kg diet culture in brackish water has the highest number of male (90%). This study proven that, the hormone was effective for masculinization in eels, It useful for masculinization in eels. Results proved that the 17α-methyltestosterone highly significant (P<0.01) effect on the Eye index (3.63 – 5.14) and Fin Index (3.03 – 4.08) of eels. This study concluded, that 17α-methyltestosterone more effective in improving the number of males in brackish water than in freshwater culture.

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Section: Resultssupporting

confidence: 90%

Effectively Of 17α-Methyltestosterone on Tropical Eel, Anguilla bicolor McClelland Masculinization in Different Salinity Culture

2018

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“…Nilai IKG ikan sidat pada masing-masing perlakuan relatif sama tidak berbeda nyata (p>0,05) masih di bawah 2%, sedangkan ikan sidat fase silver di alam mempunyai nilai IKG 2,88% (Rachmawati & Susilo 2012). Hasil penelitian Rovara et al (2008) yang menggunakan ekstrak hipofisis ikan mas dengan dosis ½, berhasil memacu kematangan gonad yang tecermin pada peningkatan nilai IKG hingga mencapai 3,37%.…”

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Karakteristik reproduksi ikan sidat Anguilla bicolor McClelland, 1844 yang diinduksi GNRH-analog

2018

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Eel, Anguilla bicolor McClelland was hard to mature in captivity, due to low levels of pituitary gonadotropin. In order to stimulate gonadal maturation in captivity, exogenous gonadotropin is needed. The purpose of this study was to assess the effect of GnRH-analog induction on gonadal maturation of tropical eel base on the value of the eye index, gonado-somatic index, and estradiol levels. The study was carried out experimentally with a completely randomized design with three treatments and eight replications. The treatments were the induction of hormone GnRH-analogues with doses of 0.0; 0.5 and 1.0 ml kg-1 body weight. Eel with an average total length of 67 cm and an average weight of 500 g were injected with different doses of GnRH-analog (0.0; 0.5 and 1.0 ml kg-1 body weight), kept in the fiberglass aquarium for two months. The eels were fed with discard fish daily in ad libitum at 16.00 pm. The observed variables were eye index, Gonadosomatic Index (GSI), and estradiol levels. The results showed that injection of three different doses of GnRH-analog did not affect on the increasing of GSI, eye index (P> 0.05), and estradiol (P <0.05). Induction of GnRH-analog of 0.0; 0.5 and 1.0 ml kg-1 body weight can not stimulate the gonadal maturity of tropical eel. AbstrakIkan sidat Anguilla bicolor McClelland sulit matang gonad dalam kondisi budi daya, karena rendahnya kadar gonado-tropin pituitari. Oleh karena itu untuk memacu pematangan gonad ikan sidat dalam kondisi budi daya perlu ditambah-kan gonadotropin secara eksogen. Tujuan penelitian ini adalah mengkaji pengaruh induksi GnRH-analog terhadap pe-matangan gonad ikan sidat berdasarkan perubahan nilai indeks mata, indeks kematangan gonad (IKG), dan kadar estradiol. Penelitian dilakukan secara eksperimental menggunakan rancangan acak lengkap dengan tiga perlakuan dan delapan ulangan. Perlakuan percobaan terdiri atas induksi hormon GnRH-analog dengan dosis 0,0; 0,5 dan 1,0 ml kg-1 bobot badan. Ikan sidat dengan rata-rata ukuran panjang 67 cm dan rata-rata bobot 500 g sebanyak 18 ekor yang telah diinduksi dengan berbagai dosis GnRH analog (0, 0,5 dan 1 ml kg-1 bobot badan) dipelihara dalam akuarium fiberglass selama dua bulan. Pakan berupa ikan rucah diberikan satu kali sehari pada pukul 16.00 secara ad libitum. Variabel yang diamati adalah indeks mata, IKG, dan kadar estradiol. Hasil penelitian menunjukkan bahwa induksi hormon GnRH-analog tidak berpengaruh terhadap peningkatan nilai indeks mata, nilai IKG (p>0,05), dan kadar estradiol ikan sidat (p<0,05). Induksi hormon GnRH-analog dengan dosis 0,5 dan 1 ml kg-1 bobot badan tidak efektif memacu pematangan gonad ikan sidat.

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“…Sintesis vitelogenin di hati sangat dipengaruhi oleh estradiol-17β oosit bertahap termasuk ke dalam tipe asinkron (metakrom) atau tidak sinkron yang berarti ovarium mempunyai oosit dengan berbagai tingkat perkembangan. Rachmawati (2012) mendeskripsikan pola perkembangan oosit pada fase yellow eel, ovarium masih dalam tahap perkembangan yang ditandai dengan posisi inti yang mulai bergerak ke tepi dan belum tampak adanya vesikula dalam sitoplasma. Hal ini terlihat pada Gambar 4 (a) di mana dapat diamati perkembangan oosit tahap 1.…”

Section: Indeks Gonadosomatik (Igs)unclassified

Evaluation of PMSG (Oodev®) application on hepatosomatic and gonadosomatic index of snakehead fish

Hutagalung

Widodo

Faqih

2015

Jurnal Akuakultur Indonesia

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ABSTRACT Snakehead fish (Channa striata) is a freshwater fish commodities that have high economic value, while their needs depend on natural catching. In general, hormonal induction commonly used for the efficiency of female parent utilization and enhancement quality and quantity of fish fry. One of the hormones that can be used is pregnant mare serum gonadotropin (PMSG) and antidopamine with trademark Oodev®. These hormones contain many elements follicle stimulating hormone (FSH) which can trigger the early stages of gonad maturity. The purpose of this research was to determine the effectiveness of hormone PMSG on gonadosomatic index (GSI) and hepatosomatic index (HSI). The size of female fish was 30−40 cm, weighing of 500−700 g, adapted in the aquarium and then induced with different doses of PMSG, namely: treatment A dose: 0.75 mL/kg; B: 1.0 mL/kg; C: 1.25 mL/kg; D: 1.5 mL/kg and be repeated three times. Fishes were then reared up to 72 hours to determine the development of GSI and HSI. The results showed that the best treatment was at dose of 1.25 mL/kg with GSI and HSI values of 1.37% and 3.35%, respectively. Keywords: PMSG, GSI, HSI, snakehead fish ABSTRAK Ikan gabus (Channa striata) merupakan salah satu komoditas air tawar yang mempunyai nilai ekonomi tinggi. Saat ini pemenuhan kebutuhan hanya bergantung pada hasil penangkapan dari alam. Pada umumnya cara pemijahan buatan dengan induksi hormon dilakukan untuk efisiensi penggunaan induk serta peningkatan kualitas dan kuantitas benih ikan. Salah satu hormon yang dapat digunakan adalah pregnant mare serum gonadotropin (PMSG) dan antidopamin dengan merk dagang Oodev®. Hormon-hormon tersebut banyak mengandung unsur follicle stimulating hormon (FSH) yang dapat memicu kematangan gonad tahap awal. Tujuan dari penelitian ini adalah untuk menguji efektivitas hormon PMSG terhadap nilai indeks gonadosomatik (IGS) dan indeks hepatosomatik (IHS). Induk ikan gabus yang digunakan berukuran 30−40 cm dengan bobot 500–700 g, diaklimatisasikan di akuarium kemudian diinduksi hormon PMSG dengan perlakuan dosis A: 0,75 mL/kg; B: 1,0 mL/kg; C: 1,25 mL/kg; D: 1,5 mL/kg dan dilakukan pengulangan sebanyak tiga kali. Selanjutnya ditunggu hingga 72 jam untuk mengevaluasi perkembangan IGS dan IHS nya. Hasil penelitian menunjukkan bahwa perlakuan terbaik adalah dosis 1,25 ml/kg dengan nilai rata-rata IHS 1,37% dan IGS 3,35%, Kata kunci: PMSG, IGS, IHS, ikan gabus

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KAJIAN HISTOLOGI OVARIUM IKAN SIDAT, Anguilla bicolor McClelland, YANG TERTANGKAP DI SEGARA ANAKAN CILACAP

Cited by 9 publications

References 6 publications

Effectively Of 17α-Methyltestosterone on Tropical Eel, Anguilla bicolor McClelland Masculinization in Different Salinity Culture

Effectively Of 17α-Methyltestosterone on Tropical Eel, Anguilla bicolor McClelland Masculinization in Different Salinity Culture

Karakteristik reproduksi ikan sidat Anguilla bicolor McClelland, 1844 yang diinduksi GNRH-analog

Evaluation of PMSG (Oodev®) application on hepatosomatic and gonadosomatic index of snakehead fish

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