Kainic acid-induced seizures in the common marmoset

Ishikawa, Akiyoshi; Mizuno, Yuri; Sakai, Keita; Maki, Takehiro; Tanaka, Ryo; Oda, Yasuhiro; Niimi, Kimie; Takahashi, Eiki

doi:10.1016/j.bbrc.2020.02.121

Cited by 2 publications

(3 citation statements)

References 22 publications

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“…The seizure behaviors were evaluated based on revised Racine’s seizure scale for marmoset proposed in previous study [12, 15] . The seizure activities of marmosets were classified as follows: Mouth cleaning-like behavior (rubbing the face along the perch and wire mesh); Head clonus/shaking (rapid and violent movement of the head); Forelimb clonus; Bilateral forelimb clonus, straub tail, postural impairment; Generalized clonic seizures. …”

Section: Methodsmentioning

confidence: 99%

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A natural marmoset model of genetic generalized epilepsy

Yang

Chen

Wang

et al. 2021

Preprint

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SummaryAs a common neurological disease, epilepsy has been extensively studied. Efforts have been made on rodent and other animal models to reveal the pathogenic mechanisms of epilepsy and develop new drugs as treatment. However, the features of current epilepsy models cannot fully mimic different kinds of epilepsy in human, asking for non-human primates models of epilepsy. The common marmoset (Callithrix jacchus) is a New World monkey that is widely used to study brain function. Here, we show a natural marmoset model of generalized epilepsy. In this unique marmoset family, generalized epilepsy was successfully induced by handling operation in some individuals. We mapped the marmoset family with handling-sensitive epilepsy and found that epileptic marmoset had an autosomal dominant genetic predisposition. Those marmosets were more sensitive to epilepsy inducers pentylenetetrazol (PTZ). By electrocorticogram (ECoG) recording, we detected epileptic discharge in marmoset with history of seizures. However, there was no significant change in the overall structure of epileptic marmoset brain. In summary, we report a family of marmosets with generalized seizures induced by handling operation. This epileptic marmoset family provides insights to better understand the mechanism of generalized epilepsy and helps to develop new therapeutic methods.Short summaryDespite the rodent and other animal models on epilepsy, a common neurological disease, the understanding of the pathogenic mechanisms of epilepsy and treatment are yet to be advanced., Non-human primates are good experimental animals for modelling neurological disease. Here we report a common marmoset family with generalized epilepsy induced by handling stimulation. We mapped the epileptic marmoset family and found that epilepsy had a genetic predisposition. The present report deals in detail with the phenotypes and characteristics of epileptic marmosets. This natural marmoset reflect epilepsy model will help to better understand the mechanism of epilepsy and develop new therapeutic methods.

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Section: Methodsmentioning

confidence: 99%

“…The seizure behaviors were evaluated based on revised Racine's seizure scale for marmoset proposed in previous study [12,15] . The seizure activities of marmosets were classified as follows:…”

Section: Seizure Classificationmentioning

confidence: 99%

A natural marmoset model of genetic generalized epilepsy

Yang

Chen

Wang

et al. 2021

Preprint

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“…After administration of KA, mouse behavior was observed for 3 h. Seizure activity was assessed according to Racine’s classification [ 21 ]: phase 1, facial clonus; phase 2, nodding; phase 3, forelimb clonus; phase 4, forelimb clonus with rearing; phase 5, rearing, jumping, and falling. Phases 1 to 3 and 4 to 5 were considered focal seizures and generalized seizures, respectively [ 22 ]. Mice that reached phases 4 to 5 and maintained that level for at least 30 min were considered to have experienced SE.…”

Section: Methodsmentioning

confidence: 99%

Pretreatment with Methylene Blue Protects Against Acute Seizure and Oxidative Stress in a Kainic Acid-Induced Status Epilepticus Model

et al. 2021

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Background The aim of the present study was to investigate the potential anticonvulsant effect of methylene blue (MB) in a kainic acid (KA)-induced status epilepticus (SE) model. The effects of MB on levels of oxidative stress and glutamate (Glu) also were explored. Material/Methods Sixty C57BL/6 mice were randomly divided into 5 equal-sized groups: (1) controls; (2) KA; (3) MB 0.5 mg/kg+KA; (4) MB 1 mg/kg+KA; and (5) vehicle+KA. The SE model was established by intra-amygdala microinjection of KA. Behavioral observations and simultaneous electroencephalographic records of the seizures in different groups were analyzed to determine the potential anticonvulsant effect of MB. The influences of MB on oxidative stress markers and glutamate were also detected to explore the possible mechanism. Results MB afforded clear protection against KA-induced acute seizure, as measured by the delayed latency of onset of generalized seizures and SE, decreased percentage of SE, and increased survival rate in mice with acute epilepsy. MB markedly increased the latency to first onset of epileptiform activity and decreased the average duration of epileptiform events, as well as the percentage of time during which the epileptiform activity occurred. Administration of MB prevented KA-induced deterioration of oxidative stress markers and Glu. Conclusions MB is protective against acute seizure in SE. This beneficial effect may be at least partially related to its potent antioxidant ability and influence on Glu level.

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Kainic acid-induced seizures in the common marmoset

Cited by 2 publications

References 22 publications

A natural marmoset model of genetic generalized epilepsy

A natural marmoset model of genetic generalized epilepsy

Pretreatment with Methylene Blue Protects Against Acute Seizure and Oxidative Stress in a Kainic Acid-Induced Status Epilepticus Model

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