Kaempferol reduces obesity, prevents intestinal inflammation, and modulates gut microbiota in high-fat diet mice

Bian, Yifei; Lei, Jiaqi; Zhong, Jia; Wang, Bo; Wan, Yan; Li, Jinxin; Liao, Chaoyong; He, Yang; Liu, Zhongjie; Ito, Koichi; Zhang, Bingkun

doi:10.1016/j.jnutbio.2021.108840

Cited by 104 publications

(60 citation statements)

References 43 publications

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“…(2) A continuous subcutaneous low-rate infusion of LPS induced most, if not all, of the features of metabolic diseases. (3) Diverse nutritional interventions alleviated chronic inflammation by changing the composition of gut microbes, especially gram-negative bacteria ( 7 – 9 ). To verify this hypothesis, we used antibiotic intervention to suppress the total amount of intestinal microbes and then reduced the production of LPS.…”

Section: Discussionmentioning

confidence: 99%

“…The initial inducement of chronic inflammation is still under discussion, and no unanimous point of view is formed. It is reported that nutritional intervention alleviates the low-grade chronic inflammation by disturbing the intestinal microbes, especially gram-negative bacteria ( 7 – 9 ). Lipopolysaccharide (LPS), a product sourced from intestinal microbes and characterized as a potent mediator for inflammation, infiltrates into the lymphatic circulation after high-fat diets (HFD) is consumed ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

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The Mechanism of Lipopolysaccharide Escaping the Intestinal Barrier in Megalobrama amblycephala Fed a High-Fat Diet

et al. 2022

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With the popularity of western food characterized by excessive fat and sugars, obesity has currently been a public health issue. Low-grade chronic inflammation accompanied by obesity increases the risk of multiple epidemics such as diabetes, cancer and cardiovascular diseases. Here, we show that feeding Megalobrama amblycephala with a high-fat diet (HFD) drives obesity-related chronic inflammation and the penetration of lipopolysaccharide (LPS). Interference with antibiotics inhibits the produce of LPS and this alleviates the sustained release of pro-inflammatory factors induced by HFD. LPS penetration is attributed to weakened intestinal mucus barrier after high-fat exposure. Mechanically, the consumption of HFD inhibits the secretion of mucin 2 (MUC2) due to the induction of endoplasmic reticulum stress mediated by the inositol-requiring enzyme 1 (IRE1) /X box-binding protein 1 (XBP1) pathway in goblet cells. Furthermore, excessive lipid exacerbates the leakage of LPS across the intestinal epithelial cell barrier via the transcellular pathway. Mechanically, lipid increases the internalization of LPS in intestinal epithelial cells depending on the activation of fatty acid translocase (FAT/CD36). These results demonstrate that HFD causes the penetration of LPS due to the weakened intestinal mucosal barrier and the assistance of CD36.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Mechanism of Lipopolysaccharide Escaping the Intestinal Barrier in Megalobrama amblycephala Fed a High-Fat Diet

et al. 2022

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“…Quercetin and kaempferol have been revealed to ameliorate GI function in the presence of IBS for their ability to inhibit lipopolysaccharide and NF-kB signaling [37,38], respectively. Moreover, administration of quercetin and kaempferol was shown to ameliorate gut the microbiota composition affected by colitis or high fat diet-induced obesity, to highlight the wide range of beneficial biological actions mediated by these flavonoids [39,40].…”

Section: Discussionmentioning

confidence: 99%

Influence of Tilia tomentosa Moench Extract on Mouse Small Intestine Neuromuscular Contractility

et al. 2021

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Functional gastrointestinal disorders (FGIDs) are characterized by abdominal pain, bloating and bowel disturbances. FGID therapy is primarily symptomatic, including treatment with herbal remedies. Flower extract of Tilia tomentosa Moench (TtM) is occasionally used as an anti-spasmodic in popular medicine. Since its effect on intestinal response is unknown, we evaluated the influence of TtM extract on small intestine contractility. Ileal preparations from C57BL/6J mice were mounted in organ baths to assess changes in muscle tension, following addition of TtM extract (0.5–36 μg/mL) or a vehicle (ethanol). Changes in contractile response to receptor- and non-receptor-mediated stimuli were assessed in ileal preparations pretreated with 12 μg/mL TtM. Alterations in the enteric nervous system neuroglial network were analyzed by confocal immunofluorescence. Increasing addition of TtM induced a marked relaxation in ileal specimens compared to the vehicle. Pretreatment with TtM affected cholinergic and tachykininergic neuromuscular contractions as well as K+-induced smooth muscle depolarization. Following incubation with TtM, a significant reduction in non-adrenergic non-cholinergic-mediated relaxation sensitive to Nω-Nitro-L-arginine methyl ester hydrochloride (pan-nitric oxide synthase inhibitor) was found. In vitro incubation of intestinal specimens with TtM did not affect the myenteric plexus neuroglial network. Our findings show that TtM-induced intestinal relaxation is mediated by nitric oxide pathways, providing a pharmacological basis for the use of TtM in FGIDs.

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“…Green tea extract, which is rich in catechins, especially EGCG, has been shown to improve the integrity of the intestinal barrier by reducing the translocation of gut-derived endotoxin and thus the resulting proinflammatory response in both animals and humans [87][88][89]. Campferol, which is present in tea leaves, improves the integrity of the intestinal barrier and inhibits inflammation in the intestines by reducing the activation of the TLR4/NF-κB pathway, and prevents the obesity-related gut dysbiosis [90].…”

Section: Teamentioning

confidence: 99%

The Role of Nutritional Factors in the Modulation of the Composition of the Gut Microbiota in People with Autoimmune Diabetes

et al. 2022

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Type 1 diabetes mellitus (T1DM) is a disease marked by oxidative stress, chronic inflammation, and the presence of autoantibodies. The gut microbiota has been shown to be involved in the alleviation of oxidative stress and inflammation as well as strengthening immunity, thus its’ possible involvement in the pathogenesis of T1DM has been highlighted. The goal of the present study is to analyze information on the relationship between the structure of the intestinal microbiome and the occurrence of T1DM. The modification of the intestinal microbiota can increase the proportion of SCFA-producing bacteria, which could in turn be effective in the prevention and/or treatment of T1DM. The increased daily intake of soluble and non-soluble fibers, as well as the inclusion of pro-biotics, prebiotics, herbs, spices, and teas that are sources of phytobiotics, in the diet, could be important in improving the composition and activity of the microbiota and thus in the prevention of metabolic disorders. Understanding how the microbiota interacts with immune cells to create immune tolerance could enable the development of new therapeutic strategies for T1DM and improve the quality of life of people with T1DM.

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Kaempferol reduces obesity, prevents intestinal inflammation, and modulates gut microbiota in high-fat diet mice

Cited by 104 publications

References 43 publications

The Mechanism of Lipopolysaccharide Escaping the Intestinal Barrier in Megalobrama amblycephala Fed a High-Fat Diet

The Mechanism of Lipopolysaccharide Escaping the Intestinal Barrier in Megalobrama amblycephala Fed a High-Fat Diet

Influence of Tilia tomentosa Moench Extract on Mouse Small Intestine Neuromuscular Contractility

The Role of Nutritional Factors in the Modulation of the Composition of the Gut Microbiota in People with Autoimmune Diabetes

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