Kaempferol attenuates mitochondrial dysfunction and oxidative stress induced by H2O2 during porcine embryonic development

Yao, Xuerui; Jiang, Hao; NanXu, Yong; Piao, Xuanjing; Gao, Qingshan; Kim, Nam‐Hyung

doi:10.1016/j.theriogenology.2019.06.013

Cited by 23 publications

(17 citation statements)

References 34 publications

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“…The quality of oocytes is a cornerstone of embryonic development, and the oxidative stress caused by in vitro culture is one of the key problems that oocytes cannot avoid. Based on previous studies, we found that many reports had investigated the addition of antioxidants such as mogroside, phloridin, or kaempferol to the in vitro culture process to combat oxidative stress, but the effects were not satisfactory [19][20][21]. It is currently recognized that the antioxidant effects of melatonin can improve the nuclear and qualitative maturation of oocytes.…”

Section: Discussionmentioning

confidence: 99%

α-Ketoglutarate Improves Meiotic Maturation of Porcine Oocytes and Promotes the Development of PA Embryos, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway

Chen

Gao

et al. 2022

Oxidative Medicine and Cellular Longevity

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α-Ketoglutarate (α-KG) is a metabolite in the tricarboxylic acid cycle. It has a strong antioxidant function and can effectively prevent oxidative damage. Previous studies have shown that α-KG exists in porcine follicles, and its content gradually increases as the follicles grow and mature. However, the potential mechanism of supplementation of α-KG on porcine oocytes during in vitro maturation (IVM) has not yet been reported. The purpose of this study was to explore the effect of α-KG on the early embryonic development of pigs and the mechanisms underlying these effects. We found that α-KG can enhance the development of early pig embryos. Adding 20 μM α-KG to the in vitro culture medium significantly increased the rate of blastocyst formation and the total cell number. Compared with to that of the control group, apoptosis in blastocysts of the supplement group was significantly reduced. α-KG reduced the production of reactive oxygen species and glutathione levels in cells. α-KG not only improved the activity of mitochondria but also inhibited the occurrence of apoptosis. After supplementation with α-KG, pig embryo pluripotency-related genes (OCT4, NANOG, and SOX2) and antiapoptotic genes (Bcl2) were upregulated. In terms of mechanism, α-KG activates the Nrf2/ARE signaling pathway to regulate the expression of antioxidant-related targets, thus combating oxidative stress during the in vitro culture of oocytes. Activated Nrf2 promotes the transcription of Bcl2 genes and inhibits cell apoptosis. These results indicate that α-KG supplements have a beneficial effect on IVM by regulating oxidative stress during the IVM of porcine oocytes and can be used as a potential antioxidant for IVM of porcine oocytes.

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Section: Discussionmentioning

confidence: 99%

α-Ketoglutarate Improves Meiotic Maturation of Porcine Oocytes and Promotes the Development of PA Embryos, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway

Chen

Gao

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…The intracellular oxidative and antioxidative statuses are primarily determined by ROS and GSH activity. Many studies have reported changes in the oxidant‐antioxidant balance following the addition of H 2 O 2 to IVM medium (Nguyen et al, 2017; Truong & Gardner, 2017; Tu et al, 2016; Yao et al, 2019). External oxygen or an inefficient antioxidant system increases ROS generation in vitro (Armand et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

The antioxidant dieckol reduces damage of oxidative stress‐exposed porcine oocytes and enhances subsequent parthenotes embryo development

Pyeon

Lee

Yoon

et al. 2021

Molecular Reproduction Devel

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This study investigated the effect of the antioxidant dieckol, a component of Ecklonia cava, on maturation and developmental competence of porcine oocytes exposed to oxidative stress in vitro. Oocytes were matured in in vitro maturation (IVM) medium containing various concentrations of dieckol. The blastocyst formation rate was highest in the 0.5 μM dieckol‐treated (0.5 DEK) group. The reactive oxygen species level was decreased, and the level of glutathione and expression of antioxidant genes (NFE2L, SOD1, and SOD2) at metaphase II were increased in the 0.5 DEK group. Abnormal spindle organization and chromosome misalignment were prevented in the 0.5 DEK group. Expression of maternal markers (CCNB1 and MOS) and activity of p44/42 mitogen‐activated protein kinase were increased in the 0.5 DEK group. After parthenogenetic activation, the total number of cells per blastocyst was increased and the percentage of apoptotic cells was decreased in the 0.5 DEK group. Expression of development‐related genes (CX45, CDX2, POU5F1, and NANOG), antiapoptotic genes (BCL2L1 and BIRC5), and a proapoptotic gene (CASP3) were altered in the 0.5 DEK group. These results indicate that the antioxidant dieckol improves IVM and subsequent development of porcine oocytes and can be used to improve the quality of oocytes under peroxidation experimental conditions.

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“…Nobiletin attenuates palmitate-induced ROS and mitochondrial dysfunction in cultured alpha mouse liver 12 cells [101]. In addition, naringenin [102], luteolin [103], genistein [104], kaempferol [105], and quercetin [106] have all been observed to inhibit oxidative damage in a variety of ways. Therefore, flavonoids may eliminate oxidative damage in senescent cells and help cells to overcome aging and aging-related diseases.…”

Section: Clinical Applications Of Flavonoid On Agingmentioning

confidence: 99%

Flavonoids—Natural Gifts to Promote Health and Longevity

Fan

Yang

et al. 2022

IJMS

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The aging of mammals is accompanied by the progressive atrophy of tissues and organs and the accumulation of random damage to macromolecular DNA, protein, and lipids. Flavonoids have excellent antioxidant, anti-inflammatory, and neuroprotective effects. Recent studies have shown that flavonoids can delay aging and prolong a healthy lifespan by eliminating senescent cells, inhibiting senescence-related secretion phenotypes (SASPs), and maintaining metabolic homeostasis. However, only a few systematic studies have described flavonoids in clinical treatment for anti-aging, which needs to be explored further. This review first highlights the association between aging and macromolecular damage. Then, we discuss advances in the role of flavonoid molecules in prolonging the health span and lifespan of organisms. This study may provide crucial information for drug design and developmental and clinical applications based on flavonoids.

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Kaempferol attenuates mitochondrial dysfunction and oxidative stress induced by H2O2 during porcine embryonic development

Cited by 23 publications

References 34 publications

α-Ketoglutarate Improves Meiotic Maturation of Porcine Oocytes and Promotes the Development of PA Embryos, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway

α-Ketoglutarate Improves Meiotic Maturation of Porcine Oocytes and Promotes the Development of PA Embryos, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway

The antioxidant dieckol reduces damage of oxidative stress‐exposed porcine oocytes and enhances subsequent parthenotes embryo development

Flavonoids—Natural Gifts to Promote Health and Longevity

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