Kaempferol and quercetin isolated from Euonymus alatus improve glucose uptake of 3T3-L1 cells without adipogenesis activity

Fang, Xiankang; Gao, Jie; Zhu, Daling

doi:10.1016/j.lfs.2007.12.021

Cited by 303 publications

(196 citation statements)

References 36 publications

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“…30 Kaempferol and kaempferol 3-neohesperidoside (the avonoid glycoside) showed insulinomimetic effects and stimulation of glucose uptake in differentiated 3T3-L1 adipocytes. 31,32 Conversely, in our study no signicant differences among the experimental groups were found in the expression of GLUT4 and InsR in adipose tissue. However, although not statistically signicant, a slight increase in the betatrophin gene expression of supplemented groups was found.…”

Section: Discussioncontrasting

confidence: 71%

Modulation of hyperglycemia and TNFα-mediated inflammation by helichrysum and grapefruit extracts in diabetic db/db mice

et al. 2014

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Type-2 diabetes is associated with a chronic low-grade systemic inflammation accompanied by an increased production of adipokines/cytokines by obese adipose tissue. The search for new antidiabetic drugs with different mechanisms of action, such as insulin sensitizers, insulin secretagogues and aglucosidase inhibitors, has directed the focus on the potential use of flavonoids in the management of type-2 diabetes. Thirty six diabetic male C57BL/6J db/db mice were fed a standard diet and randomly assigned into four experimental groups: non-treated control, (n ¼ 8); acarbose (5 mg per kg bw, n ¼ 8);helichrysum (1 g per kg bw, n ¼ 10) and grapefruit (0.5 g per kg bw, n ¼ 10) for 6 weeks. The mRNA expression in pancreas, liver and epididymal adipose tissue was determined by RT-PCR. DNA methylation was quantified in epididymal fat using pyrosequencing. Mice supplemented with helichrysum and grapefruit extracts showed a significant decrease in fasting glucose levels (p < 0.05). A possible mechanism of action could be the up-regulation of liver glucokinase (p < 0.05). The antihyperglycemic effect of both extracts was accompanied by decreased mRNA expression of some proinflammatory genes (monocyte chemotactic protein-1, tumor necrosis factor-a, cyclooxygenase-2, nuclear factorkappaB) in the liver and epididymal adipose tissue. The CpG3 site of TNFa, located 5 bp downstream of the transcription start site, showed increased DNA methylation in the grapefruit group compared with the non-treated group (p < 0.01). In conclusion, helichrysum and grapefruit extracts improved hyperglycemia through the regulation of glucose metabolism in the liver and reduction of the expression of proinflammatory genes in the liver and visceral fat. The hypermethylation of TNFa in adipose tissue may contribute to reduce the inflammation associated with diabetes and obesity.

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Section: Discussioncontrasting

confidence: 71%

Modulation of hyperglycemia and TNFα-mediated inflammation by helichrysum and grapefruit extracts in diabetic db/db mice

et al. 2014

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“…Studies have also demonstrated hypoglycemic activities of flavonoids exerted by the prevention of oxidative stress in pancreatic β-cells [91][92]. In combination with anti-inflammatory capacities, these molecules may cause an improvement in blood glucose levels and side effects of the disease [93].…”

Section: Biological Activity Of Natural Plant Componentsmentioning

confidence: 99%

Chemical and Biological Aspects of Extracts from Medicinal Plants with Antidiabetic Effects

Gushiken¹,

Beserra²,

Rozza³

et al. 2016

Rev Diabet Stud

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■ AbstractDiabetes mellitus is a chronic disease and a leading cause of death in western countries. Despite advancements in the clinical management of the disease, it is not possible to control the late complications of diabetes. The main characteristic feature of diabetes is hyperglycemia, which reflects the deterioration in the use of glucose due to a faulty or poor response to insulin secretion. Alloxan and streptozotocin (STZ) are the chemical tools that are most commonly used to study the disease in rodents. Many plant species have been used in ethnopharmacology or to treat experimentally symptoms of this disease. When evaluated pharmacologically, most of the plants employed as antidiabetic substances have been shown to exhibit hypoglycemic and antihyperglycemic activities, and to contain chemical constituents that may be used as new antidiabetic agents. There are many substances extracted from plants that offer antidiabetic potential, whereas others may result in hypoglycemia as a side effect due to their toxicity, particularly their hepatotoxicity. In this article we present an updated overview of the studies on extracts from medicinal plants, relating the mechanisms of action by which these substances act and the natural principles of antidiabetic activity.

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“…Several studies have shown that intake of foods containing kaempferol is associated with reductions in mortality, the incidence of myocardial infarction, and the incidence of cerebrovascular disease, as well as with a slightly reduced risk of coronary heart disease (Geleijnse et al, 2002;Knekt et al, 2002;Lin et al, 2006). Previously, in an in vitro study, it was shown that kaempferol ameliorates hyperglycemia by improving insulin-stimulated glucose uptake in adipocytes (Fang et al, 2008). Kaempferol also performs a beneficial role in diabetes by preventing oxidative damage in pancreatic b cells .…”

Section: +mentioning

confidence: 99%

Influence of kaempferol, a flavonoid compound, on membrane-bound ATPases in streptozotocin-induced diabetic rats

Al‐Numair

Veeramani

Alsaif

et al. 2015

Pharmaceutical Biology

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Context: Kaempferol is a flavonoid found in many edible plants (e.g. tea, cabbage, beans, tomato, strawberries, and grapes) and in plants or botanical products commonly used in traditional medicine. Numerous preclinical studies have shown that kaempferol have a wide range of pharmacological activities, including antioxidant, anti-inflammatory, anticancer, cardioprotective, neuroprotective, and antidiabetic activities. Objective: The present study investigates the effect of kaempferol on membrane-bound ATPases in erythrocytes and in liver, kidney, and heart of streptozotocin (STZ)-induced diabetic rats. Materials and methods: Diabetes was induced into adult male albino rats of the Wistar strain, by intraperitoneal administration of STZ (40 mg/kg body weight (BW)). Kaempferol (100 mg/kg BW) or glibenclamide (600 mg/kg BW) was administered orally once daily for 45 d to normal and STZinduced diabetic rats. The effects of kaempferol on membrane-bound ATPases (total ATPase, Na + /K + -ATPase, Ca 2+ -ATPase, and Mg 2+ -ATPase) activity in erythrocytes and in liver, kidney, and heart were determined. Results: In our study, diabetic rats had significantly (p50.05) decreased activities of total ATPases, Na + /K + -ATPase, Ca 2+ -ATPase, and Mg 2+ -ATPase in erythrocytes and tissues. Oral administration of kaempferol (100 mg/kg BW) or glibenclamide (600 mg/kg BW) for a period of 45 d resulted in significant (p50.05) reversal of these enzymes' activities to near normal in erythrocytes and tissues when compared with diabetic control rats. Discussion and conclusion: Thus, obtained results indicate that administration of kaempferol has the potential to restore deranged activity of membrane-bound ATPases in STZ-induced diabetic rats. Further detailed investigation is necessary to discover kaempferol's action mechanism.

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Kaempferol and quercetin isolated from Euonymus alatus improve glucose uptake of 3T3-L1 cells without adipogenesis activity

Cited by 303 publications

References 36 publications

Modulation of hyperglycemia and TNFα-mediated inflammation by helichrysum and grapefruit extracts in diabetic db/db mice

Modulation of hyperglycemia and TNFα-mediated inflammation by helichrysum and grapefruit extracts in diabetic db/db mice

Chemical and Biological Aspects of Extracts from Medicinal Plants with Antidiabetic Effects

Influence of kaempferol, a flavonoid compound, on membrane-bound ATPases in streptozotocin-induced diabetic rats

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