K252a, an Indrocarbazole Derivative, Causes the Membrane of Myoblasts to Enter a Fusion-Capable State

Hirayama, Etsuko; Sasao, Nagako; Yoshimasu, Sakura; Kim, Jeman

doi:10.1006/bbrc.2001.5327

Cited by 7 publications

(5 citation statements)

References 23 publications

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“…One possible explanation is that cholesterol-enriched membrane microdomains restrict myoblast recognition, and that fusion can only occur when the domains are disorganized or internalized, the membrane becomes more fluid, and adhesion molecules (such as cadherins) can form fusion-competent complexes. Previous data showing that a decrease in membrane cholesterol is necessary for myoblast fusion (Sekiya et al 1984;Hirayama et al 2001;Nakanishi et al 2001) are in agreement with our results. The hypothesis of the internalization of cholesterolenriched microdomains participating in myoblast fusion also corroborates the work of Kalderon and Gilula (1979).…”

Section: Resultssupporting

confidence: 94%

“…Cholesterol is a sterol lipid that plays pleiotropic roles in plasma membrane function; it is involved in maintaining membrane fluidity and impermeability and the structure of lipid microdomains. A decrease in membrane cholesterol has been shown to be necessary for myoblast fusion (Sekiya et al 1984;Hirayama et al 2001;Nakanishi et al 2001). Conversely, earlier work by Cornell et al (1980) has shown that a continued local supply of cholesterol is a requirement for the assembly or maintenance of the aggregation activity that occurs concomitant with myoblast fusion.…”

Section: Introductionmentioning

confidence: 99%

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Cholesterol depletion by methyl-?-cyclodextrin enhances myoblast fusion and induces the formation of myotubes with disorganized nuclei

et al. 2004

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The formation of a skeletal muscle fiber begins with the withdrawal of committed mononucleated precursors from the cell cycle. These myoblasts elongate while aligning with each other, guided by recognition between their membranes. This step is followed by cell fusion and the formation of long striated multinucleated myotubes. We used methyl-beta-cyclodextrin (MCD) in primary cultured chick skeletal muscle cells to deplete membrane cholesterol and investigate its role during myogenesis. MCD promoted a significant increase in the expression of troponin T, enhanced myoblast fusion, and induced the formation of large multinucleated myotubes with nuclei being clustered centrally and not aligned at the cell periphery. MCD myotubes were striated, as indicated by sarcomeric alpha-actinin staining, and microtubule and desmin filament distribution was not altered. Pre-fusion MCD-treated myoblasts formed large aggregates, with cadherin and beta-catenin being accumulated in cell adhesion contacts. We also found that the membrane microdomain marker GM1 was not present as clusters in the membrane of MCD-treated myoblasts. Our data demonstrate that cholesterol is involved in the early steps of skeletal muscle differentiation.

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Section: Resultssupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Cholesterol depletion by methyl-?-cyclodextrin enhances myoblast fusion and induces the formation of myotubes with disorganized nuclei

et al. 2004

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“…Notably, these biological processes were not observed in differentially expressed genes from each pairwise comparison, elucidating that the stage‐specific expression may be responsible for the specific molecular mechanisms. During skeletal muscle development, myoblasts undergo a number of biochemical and morphological changes, including membrane cholesterol decreased during the early stages of myoblasts fusion 79–83 . For instance, previous studies had demonstrated that ABCG1 ‐ and ABCA1 ‐mediated cholesterol efflux played an important role in reducing cell plasma membrane cholesterol content and apoA‐IV encoded by APOA4 gene is known to participate in promoting cholesterol efflux 84–88 .…”

Section: Discussionmentioning

confidence: 99%

“…20,22,74,75 including membrane cholesterol decreased during the early stages of myoblasts fusion. [79][80][81][82][83] For instance, previous studies had demonstrated that ABCG1-and ABCA1-mediated cholesterol efflux played an important role in reducing cell plasma membrane cholesterol content and apoA-IV encoded by APOA4 gene is known to participate in promoting cholesterol efflux. [84][85][86][87][88] In addition, elevation in cytosolic calcium, membrane remodelling and adhesive interactions are considered to participate in the fusion of myoblasts into multinucleated myotubes.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional states and chromatin accessibility during bovine myoblasts proliferation and myogenic differentiation

Wang

et al. 2022

Cell Proliferation

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Objectives: Although major advances have been made in bovine epigenome study, the epigenetic basis for fetal skeletal muscle development still remains poorly understood. The aim is to recapitulated the time course of fetal skeletal muscle development in vitro, and explore the dynamic changes of chromatin accessibility and gene expression during bovine myoblasts proliferation and differentiation.Methods: PDGFR-cells were isolated from bovine fetal skeletal muscle, then cultured and induced myogenic differentiation in vitro in a time-course study (P, D0, D2, and D4). The assay for transposase-accessible chromatin sequencing (ATAC-seq) and RNA sequencing (RNA-seq) were performed.Results: Among the enriched transcriptional factors with high variability, we determined the effects of MAFF, ZNF384, and KLF6 in myogenesis using RNA interference (RNAi). In addition, we identified both stage-specific genes and chromatin accessibility regions to reveal the sequential order of gene expression, transcriptional regulatory, and signal pathways involved in bovine skeletal muscle development. Further investigation integrating chromatin accessibility and transcriptome data was conducted to explore cis-regulatory regions in line with gene expression. Moreover, we combined bovine GWAS results of growth traits with regulatory regions defined by chromatin accessibility, providing a suggestive means for a more precise annotation of genetic variants of bovine growth traits. Conclusion:Overall, these findings provide valuable information for understanding the stepwise regulatory mechanisms in skeletal muscle development and conducting beef cattle genetic improvement programs. | INTRODUCTIONSkeletal muscle, which accounts for about 40% of total body weight, 1 is a highly dynamic tissue of the body. The growth and maintenance of skeletal muscle play an important role in crucial Qian Li and Yahui Wang contributed equally to this work and shared the first authorship.

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“…Among all these aforementioned players, the membrane lipid cholesterol is perhaps the one we know best about its role in myoblast fusion ( van der Bosch et al, 1973 ; Cornell et al, 1980 ; Sekiya et al, 1984 ; Hirayama et al, 2001 ; Nakanishi et al, 2001 ). Cholesterol is a multifunctional molecule involved in plasma membrane fluidity and permeability, in addition to take part of the synthesis of steroid hormones and others biological processes.…”

Section: Dissecting Skeletal Myogenesis Using Chick Myoblast Culturesmentioning

confidence: 99%

The Role of Embryonic Chick Muscle Cell Culture in the Study of Skeletal Myogenesis

2021

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The mechanisms involved in the development of skeletal muscle fibers have been studied in the last 70 years and yet many aspects of this process are still not completely understood. A myriad of in vivo and in vitro invertebrate and vertebrate animal models has been used for dissecting the molecular and cellular events involved in muscle formation. Among the most used animal models for the study of myogenesis are the rodents rat and mouse, the fruit fly Drosophila, and the birds chicken and quail. Here, we describe the robustness and advantages of the chick primary muscle culture model for the study of skeletal myogenesis. In the myoblast culture obtained from embryonic chick pectoralis muscle it is possible to analyze all the steps involved in skeletal myogenesis, such as myoblast proliferation, withdrawal from cell cycle, cell elongation and migration, myoblast alignment and fusion, the assembly of striated myofibrils, and the formation of multinucleated myotubes. The fact that in vitro chick myotubes can harbor hundreds of nuclei, whereas myotubes from cell lines have only a dozen nuclei demonstrates the high level of differentiation of the autonomous chick myogenic program. This striking differentiation is independent of serum withdrawal, which points to the power of the model. We also review the major pro-myogenic and anti-myogenic molecules and signaling pathways involved in chick myogenesis, in addition to providing a detailed protocol for the preparation of embryonic chick myogenic cultures. Moreover, we performed a bibliometric analysis of the articles that used this model to evaluate which were the main explored topics of interest and their contributors. We expect that by describing the major findings, and their advantages, of the studies using the embryonic chick myogenic model we will foster new studies on the molecular and cellular process involved in muscle proliferation and differentiation that are more similar to the actual in vivo condition than the muscle cell lines.

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K252a, an Indrocarbazole Derivative, Causes the Membrane of Myoblasts to Enter a Fusion-Capable State

Cited by 7 publications

References 23 publications

Cholesterol depletion by methyl-?-cyclodextrin enhances myoblast fusion and induces the formation of myotubes with disorganized nuclei

Cholesterol depletion by methyl-?-cyclodextrin enhances myoblast fusion and induces the formation of myotubes with disorganized nuclei

Transcriptional states and chromatin accessibility during bovine myoblasts proliferation and myogenic differentiation

The Role of Embryonic Chick Muscle Cell Culture in the Study of Skeletal Myogenesis

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