K⁺(Rb⁺) and Ca²⁺ fluxes in young winter wheat plants exposed to sub‐zero temperatures

Abstract

Ice crystal formation temperature was determined in the region of the crown in one group of 7‐day‐old intact unhardened high‐salt plants of winter wheat (Triticum aestivum L. cv. Weibulls Starke II) with TA (Thermal Analysis) and DTA (Differential Thermal Analysis) methods. After exposure of another group of plants, grown for the first 7 days in the same way as the first group, to various sub‐zero temperatures (‐1 to 5°C), influx in roots of Rb+(86Rb+) and Ca2+(45Ca2+) and contents of K+ and Ca2+ were determined at intervals during 7 days of recovery. Ice crystal formation in the crown tissue was probably extracellular and took place at about ‐4°C. There was a large loss of K+ from the roots after treatment at sub‐zero temperatures. This loss increased as the temperature of the sub‐zero treatment decreased. During recovery, roots of plants exposed to ‐1, ‐2 and ‐3°C gradually reabsorbed K+. Reabsorption of K+ in roots of plants exposed to ‐4°C was greatly impaired. Rb+ influx decreased and Ca2+ influx increased after sub‐zero temperature treatments of the plants. Active Rb+ influx mechanisms and active extrusion of Ca2+ were impaired or irreversibly damaged by the exposure. While Rb+ influx mechanisms were apparently repaired during recovery in plants exposed to temperatures down to ‐3°C, Ca2+ extrusion mechanisms were not. The temperature for ice crystal formation in the region of the crown tissue coincides with the temperature at which the plants lost the ability to reabsorb K+ and to repair Rb+ influx mechanisms during the recovery period. Plants were lethally damaged at temperatures below −4°C.