We present an efficient and expandable calcium imaging analysis platform that encapsulates the entire analysis process from raw data to interactive e-figures. It provides a graphical interface to the latest analysis methods for pre-processing, and signal extraction. We demonstrate how Mesmerize can be applied to a broad range of scientific questions by using datasets ranging from the mouse visual cortex, neurons, epidermis and TLCs of the protochordate C. intestinalis, and C. elegans.Large-scale calcium imaging of neuronal activity in populated brain regions, or entire animals, has become an indispensable technique in neuroscience research. The analysis of calcium imaging datasets presents significant challenges with respect to signal extraction and data organization.Numerous commendable packages, such as the Caiman library 1 , Suite2p 2 and SIMA 3 have been created to address pre-processing and signal extraction of imaging data. However, users often incorporate these tools in custom written scripts without a system to link the analysis procedures, raw and analyzed data.This non-ideal organization can greatly impede the reproducibility of the work even when the raw data are available 4,5 . Projects such as the Jupyter notebook 6 can help address some of these issues; however these platforms are not easily accessible to non-programmers. Moreover, these solutions do not have a system in place for FAIR, open & functionally linked data 4,7,8 . In order to address these organizational