K+-Selective Inward-Rectifying Channels and Apoplastic pH in Barley Roots1

Amtmann, Anna; Jelitto, Till C.; Sanders, Dale

doi:10.1104/pp.120.1.331

Cited by 60 publications

(35 citation statements)

References 47 publications

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“…We were able to measure the current at pH values ranging from pH 7 to 4.5, the current was clearly increased as the pH of the external solution reduced, showing that the K ϩ current is acid activated. Similar findings have been recently reported with barley root protoplasts (46). The biophysical and pharmacological characteristics of the KDC1 channel in CHO cells recorded in the whole cell configuration were in good agreement with those obtained from root hair protoplasts (were Kdc1 is highly expressed).…”

Section: Discussionsupporting

confidence: 90%

KDC1, a Novel Carrot Root Hair K+Channel

Downey¹,

Szabó²,

Ivashikina³

et al. 2000

Journal of Biological Chemistry

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Potassium is an essential nutrient which plays an important role in many aspects of plant growth and development. Plants have developed a number of highly specific mechanisms to take up potassium from the soil; these include the expression of K ؉ transporters and potassium channels in root cells. Despite the fact that root epidermal and hair cells are in direct contact with the soil, the role of these tissues in K ؉ uptake is not well understood. Here we report the molecular cloning and functional characterization of a novel potassium channel KDC1 which forms part of a new subfamily of plant K in channels. Kdc1 was isolated from carrot root RNA and in situ hybridization experiments show Kdc1 to be highly expressed in root hair cells. Expressing the KDC1 protein in Chinese hamster ovary cells identified it as a voltage and pH-dependent inwardly rectifying potassium channel. An electrophysiological analysis of carrot root hair protoplasts confirmed the biophysical properties of the Kdc1 gene product (KDC1) in the heterologous expression system. KDC1 thus represents a major K ؉ uptake channel in carrot root hair cells.

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Section: Discussionsupporting

confidence: 90%

KDC1, a Novel Carrot Root Hair K+Channel

Downey¹,

Szabó²,

Ivashikina³

et al. 2000

Journal of Biological Chemistry

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“…The element considered is located at 0.2 mm above root apex at 0 time point. Reprint from Peters (2004) with the permission of the publisher (Licence No: 2693010825600, 'John Wiley and Sons') Amtmann et al, (1999) using different experimental systems had similar results on barley roots. They found that H + excretion could have crucial role in activation of inward K + channels.…”

Section: Figure 14 Trajectory Of a Root Elementmentioning

confidence: 52%

Apoplast Acidification in Growing Barley (Hordeum vulgare L.) Leaves

Visnovitz

Touati

Miller

et al. 2012

J Plant Growth Regul

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“…The finding raises the question of a potential common signal downstream of AKT1 and NRT2.1. pH is a good candidate; K-influx promotes H + -efflux through the proton pump (Amtmann et al, 1999), while nitrate influx is accompanied by H + -influx (Miller et al, 2007). The importance of apoplastic pH for extension growth and cell wall deposition is well documented, and its potential effect on MR angle is further supported by a report that root skewing is ATP dependent and reduced in aha2 mutants, defective in the major root H + -ATPase (Haruta and Sussman, 2012).…”

Section: Opposite Action Of Akt1 and Nrt21 Determines The Mr Anglementioning

confidence: 58%

Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals

et al. 2014

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As plant roots forage the soil for food and water, they translate a multifactorial input of environmental stimuli into a multifactorial developmental output that manifests itself as root system architecture (RSA). Our current understanding of the underlying regulatory network is limited because root responses have traditionally been studied separately for individual nutrient deficiencies. In this study, we quantified 13 RSA parameters of Arabidopsis thaliana in 32 binary combinations of N, P, K, S, and light. Analysis of variance showed that each RSA parameter was determined by a typical pattern of environmental signals and their interactions. P caused the most important single-nutrient effects, while N-effects were strongly light dependent. Effects of K and S occurred mostly through nutrient interactions in paired or multiple combinations. Several RSA parameters were selected for further analysis through mutant phenotyping, which revealed combinations of transporters, receptors, and kinases acting as signaling modules in K–N interactions. Furthermore, nutrient response profiles of individual RSA features across NPK combinations could be assigned to transcriptionally coregulated clusters of nutrient-responsive genes in the roots and to ionome patterns in the shoots. The obtained data set provides a quantitative basis for understanding how plants integrate multiple nutritional stimuli into complex developmental programs.

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K+-Selective Inward-Rectifying Channels and Apoplastic pH in Barley Roots1

Cited by 60 publications

References 47 publications

KDC1, a Novel Carrot Root Hair K+Channel

KDC1, a Novel Carrot Root Hair K+Channel

Apoplast Acidification in Growing Barley (Hordeum vulgare L.) Leaves

Analysis of the Root System Architecture of Arabidopsis Provides a Quantitative Readout of Crosstalk between Nutritional Signals

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