JWA as a functional molecule to regulate cancer cells migration via MAPK cascades and F-actin cytoskeleton

Chen, Hairong; Bai, Jing; Ye, Jian; Liu, Zulong; Chen, Rui; Mao, Wenge; Li, Aiping; Zhou, Jianwei

doi:10.1016/j.cellsig.2007.01.007

Cited by 62 publications

(91 citation statements)

References 41 publications

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“…We previously reported that JWA knockdown could promote B16 melanoma cell migration (Chen et al, 2007a). Here we confirmed this conclusion by using wound healing assay, transwell assay and immunofluorescence analysis.…”

Section: Jwa Inhibits Cell Attachment In Melanoma Cellssupporting

confidence: 86%

“…Our recent data showed that JWA was essential for the rearrangement of F-actin cytoskeleton and activation of MAPK cascades induced by As 2 O 3 and PMA. JWA deficiency blocked the inhibitory effect of As 2 O 3 on HeLa cell migration, but enhanced cell migration after PMA treatment (Chen et al, 2007a). As increased cell migration is a crucial step in cancer metastasis, these findings implied that JWA may regulate the metastatic potential of cancer cells.…”

Section: Discussionmentioning

confidence: 78%

“…We previously showed that JWA regulates cancer cell migration through MAPK cascades and F-actin cytoskeleton, suggesting that JWA could have an important role in cancer metastasis (Chen et al, 2007a). To further address the functional role of JWA in cancer metastasis, B16-F10 and A375, two metastatic melanoma cell lines were selected as our in vivo model.…”

Section: Jwa Knockdown Enhances Melanoma Metastasis In Vivomentioning

confidence: 99%

“…The pEGFP-C1-antisense JWA and pEGFP-C1-JWA expression plasmids were described previously (Chen et al, 2007a(Chen et al, , 2007b. The JWA siRNA expression cassette and scrambled shRNA (Huang et al, 2006) were subcloned into the linearized vector pSEC (Ambion, Austin, TX, USA) to produce JWA shRNA and control shRNA plasmids, respectively.…”

Section: Plasmids and Transfectionsmentioning

confidence: 99%

“…We previously reported that JWA regulates cancer cell migration through MAPK cascades and F-actin cytoskeleton, which suggested that JWA has a potential inhibitory role in cancer metastasis (Chen et al, 2007a).…”

Section: Introductionmentioning

confidence: 98%

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JWA regulates melanoma metastasis by integrin αVβ3 signaling

Bai

Zhang

et al. 2009

Oncogene

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JWA, a newly identified novel microtubule-associated protein (MAP), was recently demonstrated to be indispensable for the rearrangement of actin cytoskeleton and activation of MAPK cascades induced by arsenic trioxide (As 2 O 3 ) and phorbol ester (PMA). JWA depletion blocked the inhibitory effect of As 2 O 3 on HeLa cell migration, but enhanced cell migration after PMA treatment. As cancer cell migration is a hallmark of tumor metastasis and the functional role of JWA in cancer metastasis is not understood, here we show that JWA has an important role in melanoma metastasis. Our data demonstrated that JWA knockdown increased the adhesion and invasion abilities of melanoma cells. Furthermore, JWA knockdown in B16-F10 and A375 melanoma cells significantly promoted the formation and growth of metastatic colonies in vivo. Moreover, in the tumor biopsies from human melanoma patients, JWA expression was significantly decreased in malignant melanoma compared with normal nevi. In addition, we found that JWA knockdown could intensify tumor integrin a V b 3 signaling by regulating nuclear factor Sp1. These findings suggest that JWA suppresses melanoma metastasis and may serve a potential therapeutic target for human melanoma.

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Section: Jwa Inhibits Cell Attachment In Melanoma Cellssupporting

confidence: 86%

Section: Discussionmentioning

confidence: 78%

Section: Jwa Knockdown Enhances Melanoma Metastasis In Vivomentioning

confidence: 99%

Section: Plasmids and Transfectionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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JWA regulates melanoma metastasis by integrin αVβ3 signaling

Bai

Zhang

et al. 2009

Oncogene

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Downregulation of JWA promotes tumor invasion and predicts poor prognosis in human hepatocellular carcinoma

Chen

Gao

et al. 2012

Mol. Carcinog.

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We previously identified JWA as a novel microtubule-associated protein (MAP), which is implicated in carcinogenesis and tumor progression. The aims of the present study were to investigate the biological action and the prognostic significance of JWA in hepatocellular carcinoma (HCC). Quantitative real-time PCR and Western blot were used to detect JWA mRNA and protein expression, respectively, in stepwise metastatic HCC cell lines and HCC tissues. Short hairpin RNA was used to inhibit JWA expression in HCC cells. The effects of JWA depletion on cell migration, invasion, adhesion and in vivo metastasis were investigated. Immunohistochemistry of JWA was conducted in microarrays with tissue from 314 HCC patients who had undergone surgical resection. Prognostic significance was assessed using the Kaplan-Meier method and log-rank tests. The result showed JWA expression was decreased in the highly metastatic HCC cell lines and HCC tissues. Depletion of JWA caused a notable increase in cell migration, invasion and adhesion in vitro and metastasis in vivo. Furthermore, there was an inverse correlation between JWA expression and FAK expression and phosphorylation, RhoA activation and matrix metalloproteinase-2 (MMP-2) activity in HCC cells. More notably, multivariate analysis revealed that a low level of JWA expression was an independent prognosticator for both recurrence-free and overall survival for HCC patients after surgical resection, especially for AFP-normal HCC patients. Taken together, our data demonstrate that JWA plays a crucial role in HCC progression and suggest JWA may be a potential prognostic biomarker and therapeutic target for HCC.

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Drosophila melanogaster as a model to study drug addiction

2012

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Animal studies have been instrumental in providing knowledge about the molecular and neural mechanisms underlying drug addiction. Recently, the fruit fly Drosophilamelanogaster has become a valuable system to model not only the acute stimulating and sedating effects of drugs but also their more complex rewarding properties. In this review, we describe the advantages of using the fly to study drug-related behavior, provide a brief overview of the behavioral assays used, and review the molecular mechanisms and neural circuits underlying drug-induced behavior in flies. Many of these mechanisms have been validated in mammals, suggesting that the fly is a useful model to understand the mechanisms underlying addiction.

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JWA as a functional molecule to regulate cancer cells migration via MAPK cascades and F-actin cytoskeleton

Cited by 62 publications

References 41 publications

JWA regulates melanoma metastasis by integrin αVβ3 signaling

JWA regulates melanoma metastasis by integrin αVβ3 signaling

Downregulation of JWA promotes tumor invasion and predicts poor prognosis in human hepatocellular carcinoma

Drosophila melanogaster as a model to study drug addiction

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