Juvenile hormone synthesis in relation to corpus allatum development in embryos of the viviparous cockroach<i>Diploptera punctata</i>

Holbrook, Glenn L.; Chiang, Ann‐Shyn; Lee, Yuan-Ju; Lin, Cai; Schal, Coby

doi:10.1080/07924259.1998.9652343

Cited by 9 publications

(11 citation statements)

References 38 publications

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“…This was in N. cinerea at 40% post-dorsal closure (Brüning et al, 1985;Holbrook et al, 1998), but at 65% post-dorsal closure time in D. punctata. It was reported that the JH III titer peaks in embryos after 35 to 40 days of embryo development time in the B. orientalis as would be predicted under conditions used in this investigation (Short and Edwards, 1992).…”

Section: Radiochemical Assaymentioning

confidence: 81%

“…A substantial amount of methyl farnesoate (MF), the immediate precursor of JH III, has also been found in embryos of N. cinerea (Bürgin and Lanzrein, 1988;Lanzrein et al, 1984). A recent study showed that a small amount of MF is synthesized and released by embryonic CA of D. punctata by using a higher specific activity radiolabeled precursor than previous studies have used (Holbrook et al, 1998;Stay et al, 2002).…”

Section: Introductionmentioning

confidence: 94%

“…At least three broods were examined for each species. Embryos were individually dissected, and their heads were severed on a dissecting plate under cockroach saline (Holbrook et al, 1998). Upon isolating a CA-CC complex from other tissues (nerves, fat, etc.…”

Section: Radiochemical Assaymentioning

confidence: 99%

“…The complex then was transferred into 20 µl fresh radiolabeled medium in 6 × 25 mm borosilicate glass culture tubes, where the CA were incubated for 6 h at 27°C with orbital shaking. JH III and MF biosynthesis and release were measured by the procedure described by Holbrook et al (1998). Briefly, to test for synthesis of JH III and MF by insect CA (i.e., the amount retained in CA and released into medium), 100 µl of isooctane was added to each culture tube and the medium with CA was vortexed at the highest intensity.…”

Section: Radiochemical Assaymentioning

confidence: 99%

“…Broods of all species were partitioned with blunt forceps into individual embryos under cockroach saline modified to 360 mOsm/l (Holbrook et al, 1998). The day of oviposition was recorded as day 0.…”

Section: Reproductive Biology and Dorsal Closurementioning

confidence: 99%

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Juvenile hormone and methyl farnesoate production in cockroach embryos in relation to dorsal closure and the reproductive modes of different species of cockroaches

2007

Arch Insect Biochem Physiol

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Juvenile hormone (JH), produced by the corpora allata (CA), is first detectable after dorsal closure, a conspicuous event in embryogenesis. The present research found that the timing of dorsal closure was consistently at about 45% of the total embryonic development time across most of the oviparous and ovoviviparous cockroach species examined. These included the ovoviviparous cockroaches Blaberus discoidalis, Byrsotria fumigata, Rhyparobia maderae, Nauphoeta cinerea, Phoetalia pallida, Schultesia lampyridiformis, and Panchlora nivea, as well as the oviparous cockroaches Blatta orientalis, Periplaneta americana, Eurycotis floridana, and Supella longipalpa. However, the only known viviparous cockroach Diploptera punctata completed dorsal closure at 20.8% of embryo development time. Methyl farnesoate (MF), the immediate precursor of JH III, is considered a functional molecule in crustaceans; however, in insects its function is still unclear. To understand the role of JH and MF in cockroach embryos, I compared JH and MF biosynthesis and release in several cockroach species of known phylogenetic relationships. Using a radiochemical assay, the present research showed that cockroach embryos representing all three reproductive modes produced and released both JH and MF, as previously shown for B. germanica, N. cinerea, and D. punctata. Members of a pair of embryonic CA from B. discoidalis, B. fumigata, R. maderae, and D. punctata were incubated with and without farnesol. MF accumulated in large amounts only in CA of R. maderae in the presence of farnesol, which indicates that control of the last step of biosynthesis of JH, conversion of MF into JH by MF epoxidase, is probably a rate-limiting step in this species.

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Section: Radiochemical Assaymentioning

confidence: 81%

Section: Introductionmentioning

confidence: 94%

Section: Radiochemical Assaymentioning

confidence: 99%

Section: Radiochemical Assaymentioning

confidence: 99%

Section: Reproductive Biology and Dorsal Closurementioning

confidence: 99%

See 3 more Smart Citations

Juvenile hormone and methyl farnesoate production in cockroach embryos in relation to dorsal closure and the reproductive modes of different species of cockroaches

2007

Arch Insect Biochem Physiol

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Hormonal Regulation of Development and Reproduction

Palli

2016

Management of Insect Pests to Agriculture

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Juvenile hormone and allatostatins in the German cockroach embryo

Maestro

Pascual

Treiblmayr

et al. 2010

Insect Biochemistry and Molecular Biology

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Levels of juvenile hormone III (JH), FGLamide allatostatin peptides (ASTs), ASTs precursor (preproAST) mRNA and methyl farnesoate epoxidase (CYP15A1) mRNA were measured in embryos of the cockroach Blattella germanica. JH starts to rise just after dorsal closure, reaches maximal levels between 60% and 80% of embryogenesis, and decrease subsequently to undetectable levels. ASTs show low levels during the first two thirds of embryogenesis, increase thereafter and maintain high levels until hatching. PreproAST mRNA shows quite high levels during the two days following oviposition, thus behaving as a maternal transcript, the levels then become very low until mid embryogenesis, and increase afterwards, peaking towards the end of embryo development. CYP15A1 transcripts were detected around 25% embryogenesis and the levels tended to increase through embryogenesis, although differences amongst the days studied were not statistically significant. The opposite patterns of JH and AST towards the end of embryo development, along with the detection of AST immunoreactivity in corpora allata from late embryos, suggest that JH decline is caused by the increase of AST. Moreover, the uncorrelated patterns of JH concentration and CYP15A1 mRNA levels suggest that CYP15A1 expression does not modulate JH production.

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Juvenile hormone synthesis in relation to corpus allatum development in embryos of the viviparous cockroachDiploptera punctata

Cited by 9 publications

References 38 publications

Juvenile hormone and methyl farnesoate production in cockroach embryos in relation to dorsal closure and the reproductive modes of different species of cockroaches

Juvenile hormone and methyl farnesoate production in cockroach embryos in relation to dorsal closure and the reproductive modes of different species of cockroaches

Hormonal Regulation of Development and Reproduction

Juvenile hormone and allatostatins in the German cockroach embryo

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