Juvenile hormone induces the expression of the SnoSP2 gene encoding a methionine-rich hexamerin in Sesamia nonagrioides (Lepidoptera)

Gkouvitsas, Theodoros; Kourti, Anna

doi:10.1016/j.cbpb.2009.02.017

Cited by 11 publications

(4 citation statements)

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“…1a; Tables S3 and S4, Supporting information). LSPs are amino acid transport and storage molecules (Burmester 2001) regulated at the transcription level by JH (Gkouvitsas & Kourti 2009) and serve as protein sources during the provisioning of developing eggs [vitellogenesis (Pan & Telfer 2001)]. Also more highly expressed in new‐population butterflies were lipid transporters (lipophorins and perilipin ; GO:0005319; P = 8.6 × 10 −4 , Table S3, Supporting information) that are similarly involved in the mobilization and transfer of nutrients to eggs (Teixeira et al.…”

Section: Resultsmentioning

confidence: 99%

Functional genomics of life history variation in a butterfly metapopulation

et al. 2011

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In fragmented landscapes, small populations frequently go extinct and new ones are established with poorly understood consequences for genetic diversity and evolution of life history traits. Here, we apply functional genomic tools to an ecological model system, the well-studied metapopulation of the Glanville fritillary butterfly. We investigate how dispersal and colonization select upon existing genetic variation affecting life history traits by comparing common-garden reared 2-day adult females from new populations with those from established older populations. New-population females had higher expression of abdomen genes involved in egg provisioning and thorax genes involved in the maintenance of flight muscle proteins. Physiological studies confirmed that new-population butterflies have accelerated egg maturation, apparently regulated by higher juvenile hormone titer and angiotensin converting enzyme mRNA, as well as enhanced flight metabolism. Gene expression varied between allelic forms of two metabolic genes (Pgi and Sdhd), which themselves were associated with differences in flight metabolic rate, population age and population growth rate. These results identify likely molecular mechanisms underpinning life history variation that is maintained by extinction-colonization dynamics in metapopulations.

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Section: Resultsmentioning

confidence: 99%

Functional genomics of life history variation in a butterfly metapopulation

et al. 2011

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“…The quantity of mRNA levels were normalized with those of S. nonagrioides -tubulin (GenBank accession number: DQ147771) in the same samples quantified in the same manner. We have used -tubulin as a control for Real Time and semiquantitative PCR experiments and we have seen that the -tubulin mRNA was consistent in different treatments and relative to (1) the nucleic acid concentration (using Optical Density (OD) at 260 nm); and (2) the quantity of rRNA bands present in agarose gels (Gkouvitsas et al, 2008;Gkouvitsas, Kontogiannatos, & Kourti, 2009a;Gkouvitsas, Kontogiannatos, & Kourti, 2009b;Gkouvitsas & Kourti, 2009;Kontogiannatos, Michail, & Kourti, 2011;Spyliotopoulos, Gkouvitsas, Fantinou, & Kourti, 2007). The final relative mRNA levels of per and tim genes average of four independent experiments.…”

Section: Q Rt-pcrmentioning

confidence: 99%

The expression patterns of the clock genes period and timeless are affected by photoperiod in the Mediterranean corn stalk borer, Sesamia nonagrioides

Kontogiannatos

Gkouvitsas

Kourti

2016

Arch Insect Biochem Physiol

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To obtain clues to the link between the molecular mechanism of circadian and photoperiod clocks, we cloned two circadian clock genes, period (per) and timeless (tim) from the moth Sesamia nonagrioides, which undergoes facultative diapause controlled by photoperiod. Sequence analysis revealed a high degree of conservation among the compared insects fοr both genes. We also investigated the expression patterns of per and tim in brains of larvae growing under 16L:8D (long days), constant darkness (DD) and 10L:14D (short days) conditions by qPCR assays. The results showed that mRNA accumulations encoding both genes exhibited diel oscillations under different photoperiods. The oscillation of per and tim mRNA, under short-day photoperiod differed from long-day. The difference between long-day and short-day conditions in the pattern of mRNA levels of per and tim appears to distinguish photoperiodic conditions clearly and both genes were influenced by photoperiod in different ways. We infer that not all photoperiodic clocks of insects interact with circadian clocks in the same fashion. Our results suggest that transcriptional regulations of the both clock genes act in the diapause programing in S. nonagrioides. The expression patterns of these genes are affected by photoperiod but runs with 24 h by entrainment to daily environmental cues.

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“…So far, SPs and hexamerins have been cloned and studied in Musca domestica [21], Apriona germari [22,23], Apis mellifera [5], Drosophila melanogaster [24] and Reticulitermes flavipes [25]. In Lepidoptera, SPs and hexamerins have been cloned and studied in Spodoptera litura [26], Plodia interpunctella [2], Corcyra cephalonica [27], Manduca sexta [28], Amsacta albistriga [29-31], Plutella xylostella [32], Helicoverpa zea [33], Omphisa fuscidentalis [34], and Sesamia nonagrioides [35,36]. …”

Section: Introductionmentioning

confidence: 99%

Two storage hexamerins from the beet armyworm Spodoptera exigua: Cloning, characterization and the effect of gene silencing on survival

Tang

Wang

Zhang³

2010

BMC Molecular Biol

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BackgroundIn insects, hemocyanin superfamily proteins accumulate apparently to serve as sources of amino acids during metamorphosis, reproduction and development. Storage hexamerins are important members of the hemocyanin superfamily. Although insects possess storage hexamerins, very little is known about the character and specific functions of hexamerin 1 and storage protein 1 in insect development.ResultsTo gain insight into the function of storage proteins in insects, cDNAs for two storage proteins were cloned from the fat body of Spodoptera exigua. S. exigua hexamerin 1 (SeHex) cDNA contained an open reading frame of 2124 nucleotides encoding a protein of 707 amino acids with a predicted molecular weight of 82.12 kDa. S. exigua storage protein 1 (SeSP1) cDNA contained an open reading frame of 2256 bp encoding a protein of 751 amino acids with a predicted molecular weight of ~88.84 kDa. Northern blotting analyses revealed that SeHex mRNA is expressed in the fat body, cuticle, midgut and Malpighian tubules and SeSP1 in fat body, Malpighian tubules and tracheae. SeHex and SeSP1 mRNAs were expressed in fat body at different levels from first instar larvae to pupae, with expression was much lower from first instar larvae to first-day fifth instar larvae. SeHex transcript expression was high in fat body of wandering larvae (pre-pupae) and steadily decreased to the seventh pupal day. SeSP1 transcript expression was high in fat body of wandering larvae, 2-day-old fifth instar larvae and 2-, 4- and 7-day-old pupae. SeHex and SeSP1 mRNAs levels were expressed lower than control on the condition of starvation at 12 h. Of insects injected with SeHex and SeSP1 dsRNA, 38.7% and 24.3% survived to 204 h after treatment, respectively. This was significantly lower than in the controls groups.ConclusionsThese findings provide new data on the tissue distribution, expression patterns and the function in starvation of storage proteins. RNA interference results revealed that storage protein genes are key in metamorphosis, reproduction and insect development. The results for SeHex and SeSP1 interference reveal that a potential method to control this pest is to disrupt the regulation of storage proteins.

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Juvenile hormone induces the expression of the SnoSP2 gene encoding a methionine-rich hexamerin in Sesamia nonagrioides (Lepidoptera)

Cited by 11 publications

References 56 publications

Functional genomics of life history variation in a butterfly metapopulation

Functional genomics of life history variation in a butterfly metapopulation

The expression patterns of the clock genes period and timeless are affected by photoperiod in the Mediterranean corn stalk borer, Sesamia nonagrioides

Two storage hexamerins from the beet armyworm Spodoptera exigua: Cloning, characterization and the effect of gene silencing on survival

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