Juvenile hormone III-dependent conformational changes of the nuclear receptor ultraspiracle

Jones, Guilford; Woźniak, Mieczysław; Chu, Yanxia; Dhar, Subhra; Jones, Davy

doi:10.1016/s0965-1748(01)00077-7

Cited by 89 publications

(79 citation statements)

References 79 publications

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“…However, without isoform-specific USP antibodies, it is impossible to determine which USP isoform is present at the Vg EcRE during the state of arrest and after the induction of vitellogenesis by a blood meal. In addition, Drosophila USP has been reported to form homodimers in the presence of JH III in vitro (27,28). It is not clear whether AaUSP homodimers exist in the mosquito fat body at the time of elevated JH III titers.…”

Section: Discussionmentioning

confidence: 99%

Cyclicity of mosquito vitellogenic ecdysteroid-mediated signaling is modulated by alternative dimerization of the RXR homologue Ultraspiracle

Zhu

Miura

Chen

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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In anautogenous mosquitoes, egg maturation requires a blood meal. As a consequence, mosquitoes are vectors of numerous devastating human diseases. Blood feeding triggers a 20-hydroxyecdysone (20E) hormonal cascade, which activates yolk protein precursor (YPP) genes in the female fat body, an insect metabolic tissue. An important adaptation for anautogeny is the previtellogenic arrest preventing activation of YPP genes. Equally essential is termination of their expression, so that another arrest is achieved after a batch of eggs is laid. Here, we report that mosquito Seven-up (AaSvp), a chicken ovalbumin upstream promoter-transcription factor homologue, is involved in regulating the cyclicity of vitellogenic ecdysteroid-mediated signaling through heterodimerization with a retinoid X receptor homologue Ultraspiracle (USP), the obligatory functional ecdysteroid receptor (EcR) partner. AaSvp inhibits 20E-dependent activation of the vitellogenin (Vg) gene in transfection assays. Two-hybrid and GST pull-down analyses demonstrate that in vitro AaSvp interacts with both AaUSP and AaEcR. However, the coimmunoprecipitation using fat body nuclear extracts reveals that at 33-36 h postblood meal, when the 20E titer sharply declines and YPP gene expression ceases, AaSvp replaces AaEcR in USP heterodimers. The chromatin immunoprecipitation assay indicates that protein-protein interaction rather than binding competition for the Vg ecdysteroid response element accounts for the inhibition of Vg expression by AaSvp.

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Section: Discussionmentioning

confidence: 99%

Cyclicity of mosquito vitellogenic ecdysteroid-mediated signaling is modulated by alternative dimerization of the RXR homologue Ultraspiracle

Zhu

Miura

Chen

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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“…RA signal transduction in vertebrates requires the binding to and the activation of heterodimers composed of RAR and RXR nuclear receptors isoforms. Interestingly, the only insect homolog of vertebrate RXR is encoded by the Drosophila ultraspiracle (usp) gene, which has been shown to bind to JH in vitro (Jones and Sharp, 1997;Jones et al, 2001). Altogether, these data thus suggest that JH and RA have a related activity.…”

Section: Conserved Role Of Terpenoids In Organ Loopingmentioning

confidence: 98%

The retinoic-like juvenile hormone controls the looping of left-right asymmetric organs in Drosophila

2003

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In vertebrate development, the establishment of left-right asymmetry is essential for sidedness and the directional looping of organs like the heart. Both the nodal pathway and retinoic acid play major and conserved regulatory roles in these processes. We carried out a novel screen in Drosophila to identify mutants that specifically affect the looping of left-right asymmetric organs. We report the isolation of spin, a novel mutant in which the looping of the genitalia and spermiduct are incomplete; under-rotation of the genitalia indicates that spincontrols looping morphogenesis but not direction, thus uncoupling left-right asymmetry and looping morphogenesis. spin is a novel,rotation-specific allele of the fasciclin2 (Fas2) gene,which encodes a cell-adhesion protein involved in several aspects of neurogenesis. In spin mutants, the synapses connecting specific neurosecretory cells to the corpora allata are affected. The corpus allatum is part of the ring gland and is involved in the control of juvenile hormone titers during development. Our genetic and pharmacological results indicate that Fas2spin rotation defects are linked to an abnormal endocrine function and an elevated level of juvenile hormone. As juvenile hormone is an insect sesquiterpenoid related to retinoic acid, these results establish a new genetic model for studying organ looping and demonstrate an evolutionarily conserved role for terpenoids in this process.

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“…This contrasts with the functional role determined for the phospholipid molecule that occupies the ligand-binding pocket of vertebrate NR5 orphan receptors SF-1 and LRH-1 (Krylova et al, 2005). Some experimental data suggest that juvenile hormones represent the USP ligands (Jones and Sharp, 1997;Jones et al, 2002;Xu et al, 2002), but this issue still needs in vivo verification. On the other hand, in vitro and in vivo data have demonstrated that RXR-like USPs, such as TcUSP, are not activated by and do not bind RXR ligands, in contrast to original expectations from the high conservation of the residues in USP with those in RXR that interact with ligand (Iwema et al, 2007).…”

Section: Structure Of the Usp Subunitmentioning

confidence: 82%

The Structure and Function of Ecdysone Receptors

Billas

Browning

Lawrence

et al.

Ecdysone: Structures and Functions

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Juvenile hormone III-dependent conformational changes of the nuclear receptor ultraspiracle

Cited by 89 publications

References 79 publications

Cyclicity of mosquito vitellogenic ecdysteroid-mediated signaling is modulated by alternative dimerization of the RXR homologue Ultraspiracle

Cyclicity of mosquito vitellogenic ecdysteroid-mediated signaling is modulated by alternative dimerization of the RXR homologue Ultraspiracle

The retinoic-like juvenile hormone controls the looping of left-right asymmetric organs in Drosophila

The Structure and Function of Ecdysone Receptors

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