Juvenile hormone facilitates the antagonism between adult reproduction and diapause through the methoprene-tolerant gene in the female Colaphellus bowringi

Liu, Wen; Li, Yi; Zhu, Li; Zhu, Fen; Lei, Chaoliang; Wang, Xiao‐Ping

doi:10.1016/j.ibmb.2016.05.004

Cited by 89 publications

(95 citation statements)

References 48 publications

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“…In a previous study, we found that three Hsps genes, Hsp21, Hsp23 and Hsp70 , were highly expressed in diapause-destined females compared to the non-diapause-destined females, and that the absence of JH-Met signalling in non-diapause-destined females can induce the upregulation of Hsps 26. These findings suggest that Hsps play a role in stress tolerance and that JH signalling regulates their expression in C. bowringi 26. However, that study did not determine how Hsps were upregulated in diapause-destined individuals.…”

Section: Discussionmentioning

confidence: 90%

“…We first measured the expression of three Hsps, Hsp21, Hsp23 , and Hsp70 , which are highly expressed in diapausing individuals and considered critical to stress tolerance during diapause1826. There was no significant difference in the mRNA expression of Hsp21 and Hsp23 in the fat body between the dsFAS2 and dsGFP (control) groups (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…6A). Because previous research demonstrated that JH signaling was significantly higher in non-diapause-destined females, and that JH-Met signaling inhibited the expression of FAS1 to block diapause in C. bowringi 26, we investigated the effects of JH on the expression of FAS2 . The expression of FAS 2 clearly decreased in the fat body of the diapause-destined females after these received a JH analog injection (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Although Hsp70 was upregulated in adult females after these had fed for 3 days after FAS2 knockdown, we suspect that this was unrelated to stress tolerance because higher expression of Hsp70 occurred 7 days after RNAi, when beetles had entered diapause. Since there is evidence that the absence of JH is essential for reproductive diapause to occur32641, we suspect that JH-Met signalling may be the primary regulatory pathway for Hsps upregulation during diapause.…”

Section: Discussionmentioning

confidence: 99%

“…A search of our C. bowringi transcriptome database25 found two key lipid synthesis FAS genes, FAS1 and FAS2 . Our previous study suggested that the expression of FAS1 were inhibited by juvenile JH signaling and the decreased expression of FAS1 might block diapause in C. bowringi 26. We suspect that by mediating lipid reserves FAS regulates stress tolerance, the duration of the diapause preparation stage, and the incidence of diapause in diapause-destined females.…”

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confidence: 96%

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Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression

Tan

Liu

Zhu

et al. 2017

Sci Rep

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Diapause, also known as dormancy, is a state of arrested development that allows insects to survive unfavorable environmental conditions. Diapause-destined insects store large amounts of fat when preparing for diapause. However, the extent to which these accumulated fat reserves influence diapause remains unclear. To address this question, we investigated the function of fatty acid synthase (FAS), which plays a central role in lipid synthesis, in stress tolerance, the duration of diapause preparation, and whether insects enter diapause or not. In diapause-destined adult female cabbage beetles, Colaphellus bowringi, FAS2 was more highly expressed than FAS1 at the peak stage of diapause preparation. FAS2 knockdown suppressed lipid accumulation and subsequently affected stress tolerance genes expression and water content. However, silencing FAS2 had no significant effects on the duration of diapause preparation or the incidence of diapause. FAS2 transcription was suppressed by juvenile hormone (JH) and the JH receptor methoprene-tolerant (Met). These results suggest that the absence of JH-Met induces FAS2 expression, thereby promoting lipid storage in diapause-destined female beetles. These results demonstrate that fat reserves regulate stress tolerance genes expression and water content, but have no significant effect on the duration of diapause preparation or the incidence of diapause.

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Section: Discussionmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 96%

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Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression

Tan

Liu

Zhu

et al. 2017

Sci Rep

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The juvenile hormone receptor as a target of juvenoid “insect growth regulators”

Jindra

Bittova

2019

Arch Insect Biochem Physiol

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Synthetic compounds that mimic the action of juvenile hormones (JHs) are founding members of a class of insecticides called insect growth regulators (IGRs). Like JHs, these juvenoids block metamorphosis of insect larvae to reproductive adults.Many biologically active juvenoids deviate in their chemical structure considerably from the sesquiterpenoid JHs, raising questions about the mode of action of such JH mimics. Despite the early deployment of juvenoid IGRs in the mid-1970s, their molecular effect could not be understood until recent discoveries of JH signaling through an intracellular JH receptor, namely the ligand-binding transcription factor Methoprenetolerant (Met). Here, we briefly overview evidence defining three widely employed and chemically distinct juvenoid IGRs (methoprene, pyriproxyfen, and fenoxycarb), as agonist ligands of the JH receptor. We stress that knowledge of the target molecule is critical for using these compounds both as insecticides and as research tools.

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Involvement of Methoprene‐tolerant and Krüppel homolog 1 in juvenile hormone‐mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae)

Yang

Miao

et al. 2022

Arch Insect Biochem Physiol

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Methoprene‐tolerant (Met) as an intracellular receptor of juvenile hormone (JH) and the Krüppel‐homolog 1 (Kr‐h1) as a JH‐inducible transcription factor had been proved to contribute to insect reproduction. Their functions vary in different insect orders, however, they are not clear in Psocoptera. In this study, LeMet and LeKr‐h1 were identified and their roles in vitellogenesis and ovarian development were investigated in Liposcelis entomophila (Enderlein). Treatment with exogenous JH III significantly induced the expression of LeKr‐h1, LeVg, and LeVgR. Furthermore, silencing LeMet and LeKr‐h1 remarkably reduced the transcription of LeVg and LeVgR, disrupted the production of Vg in fat body and the uptake of Vg by oocytes, and ultimately led to a decline in fecundity. The results indicated that the JH signaling pathway was essential to the reproductive process of this species. Interestingly, knockdown of LeMet or LeKr‐h1 also resulted in fluctuations in the expression of FoxO, indicating the complex regulatory interactions between different hormone factors. Besides, knockdown of both LeMet and LeKr‐h1 significantly increased L. entomophila mortality. Our study provides initial insight into the roles of JH signaling in the female reproduction of psocids and provided evidence that RNAi‐mediated knockdown of Met or Kr‐h1 is a potential pest control strategy.

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Juvenile hormone facilitates the antagonism between adult reproduction and diapause through the methoprene-tolerant gene in the female Colaphellus bowringi

Cited by 89 publications

References 48 publications

Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression

Fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression

The juvenile hormone receptor as a target of juvenoid “insect growth regulators”

Involvement of Methoprene‐tolerant and Krüppel homolog 1 in juvenile hormone‐mediated vitellogenesis of female Liposcelis entomophila (End.) (Psocoptera: Liposcelididae)

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