Jute sticks biomass delignification through laccase-mediator system for enhanced saccharification and sustainable release of fermentable sugar

Suman, Sunil Kumar; Malhotra, Manisha; Kurmi, Akhilesh Kumar; Anand, N.; Bhaskar, Thallada; Ghosh, Sanjoy; Jain, Suman L.

doi:10.1016/j.chemosphere.2021.131687

Cited by 33 publications

(6 citation statements)

References 43 publications

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“…Traditional LM composting generally decomposes multifarious biopolymers for a long time to produce small compounds, resulting in low composting efficiency . Fortunately, biopolymers such as lipids, proteins, and lignocelluloses are rapidly cleaved to micromolecules such as phenols, fatty acids, amino acids, and monosaccharides by a laccase-mediator system, − thus shortening the humification process and composting time (Figure a). For example, laccase can induce the decomposition of the most recalcitrant lignin (the cleavage of C α –C β of nonphenolic sites) through a mediator-facilitated humification, which provides more “land pointing” and small humic precursors for enzymes; thus, it is conducive to the formation of humus products by laccase-driven repolymerization of these precursors …”

Section: Role Of Laccase-aided Composting In Preservation Of Organicsmentioning

confidence: 99%

Insights into the Applications of Extracellular Laccase-Aided Humification in Livestock Manure Composting

Sun

Latif

et al. 2022

Environ. Sci. Technol.

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Traditional composting is a well-suited biotechnology for onfarm management of livestock manure (LM) but still leads to the release of toxic micropollutants and imbalance of nutrients. One in situ exoenzymeassisted composting has shown promise to ameliorate the agronomical quality of end products by improving humification and polymerization. The naturally occurring extracellular laccase from microorganisms belongs to a multicopper phenoloxidase, which is verified for its versatility to tackle micropollutants and conserve organics through the reactive radical-enabled decomposition and polymerization channels. Laccase possesses an indispensable relationship with humus formation during LM composting, but its potential applications for the harmless disposal and resource utilization of LM have until now been overlooked. Herein, we review the extracellular laccase-aided humification mechanism and its optimizing strategy to maintain enzyme activity and in situ production, highlighting the critical roles of laccase in treating micropollutants and preserving organics during LM composting. Particularly, the functional effects of the formed humification products by laccaseamended composting on plant growth are also discussed. Finally, the future perspectives and outstanding questions are summarized. This critical review provides fundamental insights into laccase-boosted humification that ameliorates the quality of end products in LM composting, which is beneficial to guide and advance the practical applications of exoenzyme in humification remediation, the carbon cycle, and agriculture protection.

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Section: Role Of Laccase-aided Composting In Preservation Of Organicsmentioning

confidence: 99%

Insights into the Applications of Extracellular Laccase-Aided Humification in Livestock Manure Composting

Sun

Latif

et al. 2022

Environ. Sci. Technol.

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“…A similar effect of xylanase activity on rice straw saccharification was previously reported by Tsujiyama and Ueno [ 39 ]. Furthermore, enhanced saccharification of various lignocellulosic substrates due to the action of laccase, alone or in the presence of mediators, has been previously reported by several authors [ 5 , 18 , 40 , 41 ]. Conversely, we did not detect positive correlations between both enzymatic activities, peroxidases ( VP and MnP ) and CMCase, and the production of reducing sugars ( Table 2 ; models A and B), which is in line with previous observations [ 39 , 42 ].…”

Section: Resultsmentioning

confidence: 88%

Solid-State Fermentation of Chestnut Shells and Effect of Explanatory Variables in Predictive Saccharification Models

Pinto

Bezerra

Fraga

et al. 2022

IJERPH

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In this study, chestnut shells (CNS), a recalcitrant and low-value agro-industrial waste obtained during the peeling of Castanea sativa fruits, were subjected to solid-state fermentation by six white-rot fungal strains (Irpex lacteus, Ganoderma resinaceum, Phlebia rufa, Bjerkandera adusta and two Trametes isolates). After being fermented, CNS was subjected to hydrolysis by a commercial enzymatic mix to evaluate the effect of fermentation in saccharification yield. After 48 h hydrolysis with 10 CMCase U mL−1 enzymatic mix, CNS fermented with both Trametes strains was recorded with higher saccharification yield (around 253 mg g−1 fermented CNS), representing 25% w/w increase in reducing sugars as compared to non-fermented controls. To clarify the relationships and general mechanisms of fungal fermentation and its impacts on substrate saccharification, the effects of some independent or explanatory variables in the production of reducing sugars were estimated by general predictive saccharification models. The variables considered were lignocellulolytic activities in fungal fermentation, CNS hydrolysis time, and concentration of enzymatic hydrolysis mix. Multiple linear regression analysis revealed a very high significant effect (p < 0.0001) of fungal laccase and xylanase activities in the saccharification models, thus proving the key potential of these enzymes in CNS solid-state fermentation.

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“…In a recent work, an enzymatic extract from Trametes maxima and HOBT as mediator was used for the delignification of jute sticks. The study showed that the presence of this mediator increased porosity and surface area, favoring the attack of cellulolytic enzymes, obtaining a sugar yield of more than 20% higher compared with a control pretreated with only laccase (Suman et al 2022).…”

Section: Biohydrogenmentioning

confidence: 97%

Are biological pretreatments of lignocellulosic residues a real option for biofuels production?

Alba Fierro,

Escobedo Bretado,

Núñez Ramírez

et al. 2024

BioRes

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The use of lignocellulosic residues as feedstocks for biofuels production represents an economic and ecofriendly option, since they are generated as byproducts or wastes from different industrial areas. Nevertheless, a pretreatment method aimed at eliminating the lignin content of these residues must be performed. This is required in order to increase cellulose bioavailability, which favors the production of reducing sugars through microbial or enzymatic attack. Some performed pretreatments can be classified as physical, chemical, and physicochemical methods. Although such methods are the most used pretreatments, they are expensive and generate or make use of harmful compounds. Biological methods, by the action of microorganisms or their enzymes for lignin content reduction, may be regarded as an alternative, being cheaper and more friendly to the environment than the aforementioned methods. However, until now, biological pretreatments have not shown the same yield as the previously mentioned methods in both sugar recovery and biofuel production. In that sense, the aim of this work is to review the efficiency of these methods, with the goal of clarifying their advantages and disadvantages for improvement of biofuel production.

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Jute sticks biomass delignification through laccase-mediator system for enhanced saccharification and sustainable release of fermentable sugar

Cited by 33 publications

References 43 publications

Insights into the Applications of Extracellular Laccase-Aided Humification in Livestock Manure Composting

Insights into the Applications of Extracellular Laccase-Aided Humification in Livestock Manure Composting

Solid-State Fermentation of Chestnut Shells and Effect of Explanatory Variables in Predictive Saccharification Models

Are biological pretreatments of lignocellulosic residues a real option for biofuels production?

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