Junctions between lens cells in differentiating cultures: Structure, formation, intercellular permeability, and junctional protein expression

Menko, A. Sue; Klukas, Kathleen A.; Liu, Tai Feng; Quade, Bradley J.; Sas, Daryl F.; Preus, Doris M.; Johnson, Ross G.

doi:10.1016/0012-1606(87)90389-7

Cited by 27 publications

(18 citation statements)

References 31 publications

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“…Normal lens cultures, when examined at the electron microscopic level, are very similar to normal lens tissue (20,21). The lentoids which form as the cultures differentiate are comprised of cells which are characterized by an increased cell volume, loss of organelles, and extensive junctional membrane.…”

Section: Methodsmentioning

confidence: 95%

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Inhibition of chicken embryo lens differentiation and lens junction formation in culture by pp60v-src.

Menko¹,

Boettiger²

1988

Mol. Cell. Biol.

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A culture system was developed which permitted the differentiation of chicken lens epithelial cells to lentoid bodies which contained several cell layers, accumulated high levels of delta-crystallin, and produced extensive gap junctions. This differentiation process was prevented when the cells were infected with a temperaturesensitive src mutant of Rous sarcoma virus and maintained at the permissive temperature. These transformed cells continued to proliferate and also synthesized the major lens gap junction protein, MP28, at near-normal rates. However, this MP28 was not assembled to produce gap junctions. Cultures shifted to the nonpermissive temperature formed lentoid bodies similar to those in uninfected lens cultures, including the establishment of gap junctions containing MP28.

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Section: Methodsmentioning

confidence: 95%

“…3a, a thin section in which the entire area of plasma membrane apposition seen is junctional and only a part of an even larger junction. Previous reports have extensively characterized the junctions in normal chicken embryo lens cultures (20,21). RSV-transformed lens cells, when examined as thin sections in the electron microscope (Fig.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of chicken embryo lens differentiation and lens junction formation in culture by pp60v-src.

Menko¹,

Boettiger²

1988

Mol. Cell. Biol.

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“…First, avian embryos are very accessible for manipulation, permitting intervention and study at all stages of lens development (Beebe and Piatigorsky, 1981;Schuetze and Goodenough, 1982;Miller and Goodenough, 1986). Second, methods for the culture of embryonic avian lens cells are well developed, and the cultured cells differentiate into "lentoids", clusters of cells that express proteins unique to the differentiated lens fibers and that acquire some of the differentiated fiber morphology (Okada et al, 1971;Piatigorsky et al, 1973;Menko et al, 1984Menko et al, , 1987. Moreover, these avian cultures are capable of assembling large numbers of fiber-fiber junctions, which is not true for cultures of rat lens cells (FitzGerald and Goodenough, 1986;Jiang et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and functional characterization of chick lens fiber connexin 45.6.

Jiang

White

Goodenough

et al. 1994

MBoC

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The avian lens is an ideal system to study gap junctional intercellular communication in development and homeostasis. The lens is experimentally more accessible in the developing chick embryo than in other organisms, and chick lens cells differentiate well in primary cultures. However, only two members of the connexin gene family have been identified in the avian lens, whereas three are known in the mammalian system. We report here the molecular cloning and characterization of the third lens connexin, chick connexin45.6 (ChCx45.6), a protein with a predicted molecular mass of 45.6 kDa. ChCx45.6 was encoded by a single copy gene and was expressed specifically in the lens. There were two mRNA species of 6.4 kilobase (kb) and 9.4 kb in length. ChCx45.6 was a functional connexin protein, because expression in Xenopus oocyte pairs resulted in the development of high levels of conductance with a characteristic voltage sensitivity. Antisera were raised against ChCx45.6 and chick connexin56 (ChCx56), another avian lens-specific connexin, permitting the examination of the distribution of both proteins. Immunofluorescence localization showed that both ChCx45.6 and ChCx56 were abundant in lens fibers. Treatment of lens membranes with alkaline phosphatase resulted in electrophoretic mobility shifts, demonstrating that both ChCx45.6 and ChCx56 were phosphoproteins in vivo.

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“…The lens is composed of two major cell types: epithelial cells, which are primarily located at the anterior of the lens, and fiber cells, which are located at the cortical and posterior region (1,2). Previous studies have shown that the lens epithelial cells (LECs) differentiate into lens fiber cells, and that this is followed by the degeneration of their nuclei and organelles as development proceeds.…”

mentioning

confidence: 99%

Downregulated Expression of Integrin α6 by Transforming Growth Factor-β1 on Lens Epithelial Cells in Vitro

Lim

Kim

Lee

et al. 2001

Biochemical and Biophysical Research Communications

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Junctions between lens cells in differentiating cultures: Structure, formation, intercellular permeability, and junctional protein expression

Cited by 27 publications

References 31 publications

Inhibition of chicken embryo lens differentiation and lens junction formation in culture by pp60v-src.

Inhibition of chicken embryo lens differentiation and lens junction formation in culture by pp60v-src.

Molecular cloning and functional characterization of chick lens fiber connexin 45.6.

Downregulated Expression of Integrin α6 by Transforming Growth Factor-β1 on Lens Epithelial Cells in Vitro

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