Junctional Adhesion Mechanisms in Airway Basal Cells

Evans, Michael J.; Cox, Robert A.; Shami, Susan G.; Plopper, Charles G.

doi:10.1165/ajrcmb/3.4.341

Cited by 36 publications

(10 citation statements)

References 10 publications

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“…There has been some controversy as to whether basal cells are immature "precursors" of other bronchial epithelial cells or represent a differentiated population with specific functions [24]. The absence of cells expressing intermediate levels of 4-1BB is consistent with the later possibility and suggests that this antibody may be useful in isolating basal cells for functional studies.…”

Section: Discussionmentioning

confidence: 82%

Expression of three members of the TNF-R family of receptors (4-1BB, lymphotoxin-beta receptor, and Fas) in human lung

Boussaud¹,

Soler²,

Moreau³

et al. 1998

Eur Respir J

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Receptors belonging to the tumour necrosis factor receptor (TNF-R) superfamily are implicated in a variety of important biological processes. Although messenger ribonucleic acid coding for many of these receptors has been detected in lung, little is known about their expression in this organ. In this study, immunohistochemical techniques were used to evaluate the expression of three receptors of this family (4-1BB, lymphotoxin-beta receptor (LTbeta-R), and Fas) in normal human lung, lung carcinomas and by tuberculous and sarcoid granulomas. The 4-1BB receptor was uniformly expressed by endothelial cells of small vessels and by basal epithelial cells within pseudostratified bronchial epithelium. LTbeta-R expression by parenchymal cells was limited to those of epithelial origin (bronchial and bronchiolar epithelium, hyperplastic alveolar epithelium and lung carcinomas). Fas was present on both fibroblasts and epithelial cells (bronchial and alveolar epithelium and most, but not all, carcinomas), but not on endothelial cells. A subpopulation of T-lymphocytes expressed these receptors, but only Fas was detected on normal alveolar macrophages and epithelioid cells. Thus, 4-1BB, LTbeta-R, and Fas have characteristic and only partially overlapping patterns of expression in the lung. The findings should facilitate further evaluation of their role in lung homeostasis and pathology.

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Section: Discussionmentioning

confidence: 82%

Expression of three members of the TNF-R family of receptors (4-1BB, lymphotoxin-beta receptor, and Fas) in human lung

Boussaud¹,

Soler²,

Moreau³

et al. 1998

Eur Respir J

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“…Because the sloughed cells were intact [1 I], it was concluded that cotton smoke affected their desmosome mechanisms for attachment, which is the primary mechanism for columnar cell adherence to the basal lamina in the sheep [13,14]. In unexposed regions of the trachea, all cells appeared normal, which indicates that the effect of smoke on tracheal cells is local and not systemic.…”

Section: Discussionmentioning

confidence: 99%

Cellular sequence of tracheal repair in sheep after smoke inhalation injury

Barrow

Wang

Cox

et al. 1992

Lung

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The cellular repair process of injured tracheal epithelium is described for sheep after exposure to toxic smoke containing high concentrations of acrolein. Fourteen fasted 3-4-year-old ewes had a portion of their cervical trachea exposed to cotton smoke for 20 min and then were sacrificed at various time intervals ranging from 1 to 22 days after exposure. Within 1 day of injury, columnar epithelium sloughed intact from the trachea with a concomitant reduction of nearly 35% in the basal cell population. At 2 days of recovery, the cellularity of the epithelium had increased and mitotic figures were observed in some tracheal epithelial and gland cells. By 8 days, undifferentiated hyperplastic cells increased to 30/100 microns, differentiated nonciliated columnar cells first appeared, and the basal cell population returned to a normal count of 13 cells/100 microns. Thirteen days after exposure, the undifferentiated hyperplastic cell population had declined to 7 cells/100 microns, nonciliated columnar cells were at control values, and some ciliated cells were identified. At 18 and 22 days, epithelium was normal in appearance and the count was 13 cells/100 microns. Data suggest that because the columnar epithelium sloughs intact with the cilia remaining active, toxic smoke may affect their attachment to the basal lamina. Furthermore, the regeneration process involves differentiation of hyperplastic cells in which they elongate down to the basal lamina, thus re-establishing the integrity of tall epithelium in the sheep trachea.

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“…6,7,11). This close relationship suggests that a lesion in one component of the supracellular anchoring network may affect development of the whole network.…”

Section: Discussionmentioning

confidence: 93%

“…Collagen VII is found in the epithelial surface of the BMZ. Bar: 10 m. (6,7). In general, as the volume of the epithelium increases, the amount of junctional adhesion present in the supracellular anchoring network also increases.…”

Section: Discussionmentioning

confidence: 99%

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Reduction of collagen VII anchoring fibrils in the airway basement membrane zone of infant rhesus monkeys exposed to house dust mite

Evans

Fanucchi

Miller

et al. 2010

American Journal of Physiology-Lung Cellular and Molecular Phys

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Collagen VII anchoring fibrils in the basement membrane zone (BMZ) are part of a supracellular anchoring network that attaches the epithelium to the BMZ. Sloughing of airway epithelium in asthmatics (creola bodies) is a pathology associated with the supracellular anchoring network. In a rhesus monkey model of house dust mite (HDM)-induced allergic asthma, we found increased deposition of collagen I in the BMZ. In this study, we determine whether HDM also affected deposition of collagen VII in the BMZ. In the developing airway of rhesus monkeys, the width of collagen VII anchoring fibrils in the BMZ was 0.02 +/- 0.04 microm at 1 mo of age. At 6 mo the width had increased to 1.28 +/- 0.34 microm and at 12 mo 2.15 +/- 0.13 microm. In animals treated with HDM, we found a 42.2% reduction in the width of collagen VII layer in the BMZ at 6 mo (0.74 +/- 0.15 microm; P < 0.05). During recovery, the rate of collagen VII deposition returned to normal. However, the amount of collagen VII lost was not recovered after 6 mo. We concluded that normal development of the collagen VII attachment between the epithelium and BMZ occurs in coordination with development of the BMZ. However, in HDM-treated animals, the collagen VII attachment with the epithelium was significantly reduced. Such a reduction in collagen VII may weaken the supracellular anchoring network and be associated with sloughing of the epithelium and formation of creola bodies in asthmatics.

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Junctional Adhesion Mechanisms in Airway Basal Cells

Cited by 36 publications

References 10 publications

Expression of three members of the TNF-R family of receptors (4-1BB, lymphotoxin-beta receptor, and Fas) in human lung

Expression of three members of the TNF-R family of receptors (4-1BB, lymphotoxin-beta receptor, and Fas) in human lung

Cellular sequence of tracheal repair in sheep after smoke inhalation injury

Reduction of collagen VII anchoring fibrils in the airway basement membrane zone of infant rhesus monkeys exposed to house dust mite

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