jun:Oncogene and Transcription Factor

Cooperative cell transformation by Myc/Mil(Raf) involves induction of AP-1 and activation of genes implicated in cell motility and metastasis

“…Hence, we decided to study the regulation of the avian OPN gene Figure 1d), primary chicken embryo fibroblasts (CEF) were freshly transformed by the MH2 virus (CEF/MH2) and passaged. mRNA was isolated from these cells and analysed by Northern hybridization in comparison with mRNAs from normal CEF and from CEF transformed by the ASV17 virus carrying the v-jun oncogene (Vogt and Bos, 1990). Furthermore, secreted OPN protein (p52 OPN ) levels were analysed by immunoprecipitation using an antiserum directed against OPN recombinant protein (see below).…”

“…AP-1 represents a heterogeneous collection of dimers composed of members of the Jun, Fos, or activating transcription factor (ATF) protein families, and is a key regulator of genes involved in cell proliferation or differentiation (Curran and Franza, 1988;Ransone and Verma, 1990;Vogt and Bos, 1990;Shaulian and Karin, 2002;Vogt, 2002;Eferl and Wagner, 2003). Jun proteins can bind to DNA as functional homodimers, whereas Fos proteins need a member of the Jun protein family for dimerization (Curran and Franza, 1988;Ransone and Verma, 1990;Vogt and Bos, 1990;Vogt, 2002). AP-1 transcription factors bind with high affinity to the 12-O-tetradecanoyl-phorbol-13-acetate responsive element (TRE) with the consensus sequence 5 0 -TGA C / G TCA-3 0 , or to variants thereof, in the promoters of various target genes (Curran and Franza, 1988;Vogt and Bos, 1990;Vogt, 2002).…”

“…Jun proteins can bind to DNA as functional homodimers, whereas Fos proteins need a member of the Jun protein family for dimerization (Curran and Franza, 1988;Ransone and Verma, 1990;Vogt and Bos, 1990;Vogt, 2002). AP-1 transcription factors bind with high affinity to the 12-O-tetradecanoyl-phorbol-13-acetate responsive element (TRE) with the consensus sequence 5 0 -TGA C / G TCA-3 0 , or to variants thereof, in the promoters of various target genes (Curran and Franza, 1988;Vogt and Bos, 1990;Vogt, 2002). The oncogenic v-jun or v-fos alleles were generated by retroviral transduction of the cellular c-jun or c-fos proto-oncogenes (Curran and Franza, 1988;Vogt and Bos, 1990).…”

cJun overexpression in MCF-7 breast cancer cells produces a tumorigenic, invasive and hormone resistant phenotype

“…The role of cJun in cellular transformation has been de®ned previously in rodent and avian model systems (Vogt et al, 1990;Suzuki et al, 1991;Castellazzi et al, 1990, Dong et al, 1994. Deregulated expression of cJun can lead to malignant transformation of immortalized rat ®broblasts while transformation of primary rat embryo cells required co-expression of an activated c-Ha-ras (Alani et al, 1991;Schutte et al, 1989).…”

Bacterial expression and characterization of the CREB bZip module: Circular dichroism and 2D 1H‐NMR studies

“…Members of the leucine zipper class of proteins include the AP-1 family (Vogt & BOS, 1990); the yeast transactivator, GCN4 (Hope & Struhl, 1986; the CAAT box binding transcription factor, C/EBP (Landschulz et al, 1988); and the CREWATF family of proteins (Hai et al, 1989). The leucine zipper proteins bind to their target DNA as dimers (Kouzarides & Ziff, 1988;Landschulz et al, 1988).…”