jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun

Schütte, J.; Viallet, Jean; Nau, Marion M.; Segal, Shoshana; Fedorko, Joseph; Minna, John D.

doi:10.1016/0092-8674(89)90755-1

Cited by 450 publications

(253 citation statements)

References 56 publications

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“…The results of gel shift assays shown above indicate that forced expression of PU.1 in NIH3T3 cells leads (Chiu et al, 1989;SchuÈ tte et al, 1989). In turn, simultaneous expression of c-Jun and JunB inhibits activity of AP-1 responsive promoters due to formation of inactive c-Jun/JunB dimers (Deng and Karin, 1994).…”

Section: Forced Expression Of Pu1 In Nih3t3 Cells Results In Diminismentioning

confidence: 99%

Differential regulation of interstitial collagenase (MMP-1) gene expression by ETS transcription factors

1997

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Expression of interstitial collagenase (MMP-1) has been detected in stromal ®broblasts of various malignant tumors. Here, we have studied the e ect of three structurally di erent ETS transcription factors (ETS-1, ERGB/Fli-1, and PU.1) on MMP-1 promoter activity in NIH3T3 ®broblasts. ETS-1 increased the activity of 3.8 kb MMP-1 promoter construct up to tenfold, while ERGB/Fli-1 or PU.1 alone had no marked e ect on basal promoter activity. ETS-1 also markedly potentiated enhancement of MMP-1 promoter by both c-Jun and JunB, whereas ERGB/Fli-1 augmented only the e ect of c-Jun. Interestingly, PU.1 abolished induction of MMP-1 promoter by both c-Jun and JunB. Stimulation of MMP-1 promoter by 12-O-tetradecanoyl phorbol-13-acetate and okadaic acid was di erentially augmented by ETS-1 and ERGB/Fli-1, and abrogated by PU.1. Cotransfection studies with MMP-1 promoter 5'-deletion constructs revealed that AP-1 site was necessary for PU.1-elicited suppression. As compared to control cell lines, PU.1-positive stable cells exhibited clearly weaker binding of c-Jun and JunD containing AP-1 complexes to MMP-1 promoter AP-1 element, as well as marked reduction in basal level and induction of c-jun mRNA by 12-O-tetradecanoyl phorbol-13-acetate and okadaic acid, suggesting a novel mechanism for PU.1-mediated inhibition of AP-1 dependent gene expression. These results show that three structurally distinct ETS transcription factors di erently modulate AP-1 dependent upregulation of MMP-1 gene expression.

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Section: Forced Expression Of Pu1 In Nih3t3 Cells Results In Diminismentioning

confidence: 99%

Differential regulation of interstitial collagenase (MMP-1) gene expression by ETS transcription factors

1997

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“…Wild type c-Jun and mutants cloned into either RSV-or CMV-based eukaryotic expression vectors have been previously described: c-Jun/CMV (Brown et al, 1996); c-Jun/RSV ; JunD287 ± 331 (Alani et al, 1991); the leucine zipper point mutants, M8, M9 and M14, ; internal deletion mutants, JunD194 ± 223 and JunD146 ± 221 ; JunA?D 265 In265 (Brown et al, 1996); c-Jun/v-Jun chimeras (Oehler et al, 1993); and cJun Ala63/73 (Smeal et al, 1991). JunB (Schutte et al, 1989) and JunD (Ryder et al, 1989) have been previously described. JunD1 ± 245 was constructed by using the polymerase chain reaction (PCR) to amplify c-Jun amino acids 246 ± 331.…”

Section: Plasmidsmentioning

confidence: 99%

Identification of domains of c-Jun mediating androgen receptor transactivation

et al. 1998

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“…At least two other members, jun-B and jun-D, have been identified in the jun family. Jun-B was shown to inhibit the trans.activating activities of c-jun when both were eo-transfected into F9 cells [11][12][13][14].…”

Section: Introductioi'imentioning

confidence: 99%