Joseon Funerary Texts Tested Using Ancient DNA Analysis of a Korean Mummy

Abstract

In Korea, ancient DNA (aDNA) analysis has been applied to investigations into the genetic affiliations of mummies found in Joseon Dynasty tombs (1392( -1910, becoming now indispensable tool for researches studying human remains from archaeological sites. In the course of our recent examinations on a Korean mummy of Joseon Dynasty, we discovered many teeth contained in a pouch. And in fact, the historical literature on the topic of Joseon funerals contain general accounts of pouches in which an individual's lost teeth were collected over the course of a lifetime and, after death, placed in the coffin with the body. To test the veracity of the historical texts, the present study undertook aDNA analyses and compared the results between specifically questioned (Q) samples (teeth) and known (K) samples (brain and bone) from the mummy to ensure that they came from the same individual. Although the Q-K comparison of autosomal short tandem repeat results did not show full concordance due to allelic drop-outs in some loci, our statistical calculation indicated that the teeth in the pouch are highly likely those of the mummy. Additionally, Q-K comparison of mitochondrial DNA sequence results showed 100% matches between samples. There results, in short, Abbreviations used: aDNA 5 ancient DNA; BSA 5 bovine serum albumin;; CT 5 computed tomography; DTT 5 dithiothreitol; DVI 5 disaster victim identification; EDTA 5 ethylenediaminetetraacetic acid; HVR 5 hypervariable region; LR 5 likelihood ratio; LSMB 5 lime-soil mixture barrier; MDCT 5 multi-detector computed tomography; MPR 5 multi-planar reformat; mtDNA 5 mitochondrial DNA; PCR 5 polymerase chain reaction; SDS 5 sodium dodecyl sulfate; STR 5 short tandem repeats; VR 5 volume rendering; UV 5 ultra violet.

The impact of burial period on compact bone microstructure: Histological analysis of matrix loss and cell integrity in human bones exhumed from tropical soil

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Single-nucleotide polymorphism analyses on ABCC11, EDAR, FGFR2, and ABO genotypes of mummified people of the Joseon Dynasty, South Korea

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