Joint report1 of the First International Workshop on Lymphocyte Alloantigens of the Horse held 24–29 October 1981

Rw, Bull

doi:10.1111/j.1365-2052.1983.tb01067.x

Cited by 19 publications

(13 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of these investigations have focused on the equine lymphocyte antigen (ELA) system, which is believed to be the major histocompatibility complex (MHC) of the horse (Lizary et al, 1980a, b;Mottironi et al, 1981;Antczak et al, 1982;Bailey, 1983). Early descriptions of a complex genetic organization of the ELA region detectable by cytotoxic antisera (Bailey, 1980;Lazary et al, 1980a), have not yet been confirmed at the level of international workshops on the ELA system (Bull, 1983;.…”

Section: Introductionmentioning

confidence: 99%

Lymphocyte alloantigens of the horse. III. ELY‐2.1: a lymphocyte alloantigen not coded for by the MHC

Antczak

1984

Animal Blood Groups and Biochemical Genetics

View full text Add to dashboard Cite

Summary A new polymorphic locus of the horse which has several unusual properties is described. The suggested name for the locus is ELY‐2. The gene product of one allele at this locus, designated ELY‐2.1, has been identified with antisera raised as a result of pregnancy. Antibody to ELY‐2.1 was first detected on day 55 after conception in the serum of a mare in first pregnancy. This early onset of antibody is similar to that seen for antibody to ELA antigens, and suggests that the source of the antigenic stimulus may be the tissue of the equine endometrial cups. The antisera identifying ELY‐2.1 are cytotoxic and kill all peripheral blood lymphocytes from horses carrying the antigen. ELY‐2.1 is a cell surface molecule expressed on lymphocytes, erythrocytes, and platelets. Other cell types have not been investigated. The overall phenotypic frequency of ELY‐2.1 in several horse breeds was 16 %. The ELY‐2.1 antigen is controlled by an autosomal, dominant gene which is not coded by the ELA region (the major histocompatibility complex of the horse), nor is it identical to the ELY‐1 locus, which codes for another cell surface alloantigen of equine lymphocytes. Stimulator cells carrying ELY‐2.1 did not induce proliferation of ELY‐2.1 negative responder cells in mixed cultures of horse lymphocytes. Attempts to raise alloantisera to other alleles of the ELY‐2 locus through immunization with lymphocytes were unsuccessful. It is possible that the alternate allele(s) does not code for a gene product which is expressed. The function and biochemical nature of the ELY‐2.1 molecule are unknown.

show abstract

Section: Introductionmentioning

confidence: 99%

Lymphocyte alloantigens of the horse. III. ELY‐2.1: a lymphocyte alloantigen not coded for by the MHC

Antczak

1984

Animal Blood Groups and Biochemical Genetics

View full text Add to dashboard Cite

show abstract

“…Data from second international ELA workshop (Bailey et al, 1984). Data from first international ELA workshop (Bull, 1983). 5 Data reported by Bailey (1983).…”

Section: Discussionmentioning

confidence: 99%

“…Since then, several laboratories have performed serological and genetic studies on the major histocompatibility complex (MHC) of the horse (Bailey, 1980;Lazary et al, 1980a, b;Mottironi et al, 1981;Antczak et al, 1982). During the first international workshop on equine lymphocyte alloantigens (ELA) (Bull, 1983) an international nomenclature was accepted defining 6 allelic ELA specificities called ELA-W1, W2, W3, W4, W5 and W6. Additional ELA specificities ELA-W7, W8, W9 and W10 were accepted as a result of the second international workshop (Bailey et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Equine lymphocyte antigens in four major Belgian horse populations. Contribution to serology and antigen distribution

Varewyck

Bouquet

Lazàry

et al. 1985

Animal Blood Groups and Biochemical Genetics

View full text Add to dashboard Cite

158 Belgian Saddlebreds, 130 Belgian Trotters, 108 Belgian Draft horses and 92 Shetland ponies have been typed for serologically defined antigens at the ELA and ELY systems. Gene frequencies were estimated in each breed for the internationally established ELA, ELY-1 and ELY-2 alleles as well as for locally assigned additional ELA markers and for subtypes of ELA-W3, W9 and W11. The distribution of ELA alleles was in agreement with the expected Hardy-Weinberg equilibrium for the 4 horse breeds described here. Differences in gene frequencies between these main Belgian horse populations were observed.

show abstract

“…The MHC of the horse has been characterised sufficiently to permit typing of animals for Class I MHC antigens Bull 1983;Bailey et al 1984). These equine MHC antigens are referred to as equine lymphocyte antigens (ELA).…”

Section: Immunological Aspects Of Intraspecies Horse and Donkey Pregnmentioning

confidence: 99%

Immunological aspects of pregnancy in mules

Antczak

Davies

Kydd

et al. 1985

Equine Veterinary Journal

View full text Add to dashboard Cite

Summary The pregnant mare makes a range of immune responses to paternally‐inherited foetal histocompatibility antigens. Cytotoxic antibody is first detected in major histocompatibility complex (MHC)‐incompatible pregnancies by Days 50 to 70 of gestation. This follows shortly after the development of the endometrial cups and is concurrent with a striking accumulation of maternal lymphocytes and other mononuclear cells in the endometrial stroma around each cup. No such cellular infiltration occurs at the endometrium‐allantochorion border. The temporal coincidence between endometrial cup formation, cytotoxic alloantibody production and the accumulation of maternal lymphocytes suggests that these three events are interrelated. The relationships have been explored using immunogenetic markers in intraspecies horse pregnancy, by comparing immunological and endocrinological aspects of pregnancy in mares and jenny donkeys carrying normal intra‐specific and interspecific hybrid conceptuses and, finally, by examining foetomaternal interactions in sterile interspecies mules carrying pregnancies of both parental types created by embryo transfer. The working hypothesis has been that the endometrial cups are the source of immunogenic foetal histocompatibility antigen in equine pregnancy and that the cytotoxic antibody response and the lymphocyte accumulation around the cups represent humoral and cell‐mediated arms of the maternal immune response. Establishment of MHC‐compatible pregnancies demonstrated no significant effect of MHC differences between mother and foetus on serum equine chorionic gonadotrophin (eCG) levels, the duration of eCG secretion or the magnitude of the maternal leucocyte accumulation around the cups. Comparison of intraspecies horse and donkey pregnancies showed major differences between the two, including lower serum eCG levels, smaller endometrial cups with reduced leucocyte accumulation and a lower incidence of maternal sensitisation to foetal histocompatibility antigens in donkey pregnancy. Examination of interspecies mule and hinny pregnancies, on the other hand, showed a marked increase in the maternal lymphocyte reaction around the endometrial cups and a definite influence of the paternal genome of the foetus on eCG production. The successful transfer of horse and donkey embryos to female mules demonstrated that: (a) Both horse and donkey conceptuses express histocompatibility antigen in immunogenic form early in pregnancy; (b) both horse and donkey chorionic girdle cells can invade the endometrium of the mule to form endometrial cups, the eCG production rate of which is dependant upon foetal genotype; and (c) the magnitude of the lymphocyte accumulation around the endometrial cups does not correlate with known genetic differences between mother and foetus.

show abstract

Joint report1 of the First International Workshop on Lymphocyte Alloantigens of the Horse held 24–29 October 1981

Cited by 19 publications

References 7 publications

Lymphocyte alloantigens of the horse. III. ELY‐2.1: a lymphocyte alloantigen not coded for by the MHC

Lymphocyte alloantigens of the horse. III. ELY‐2.1: a lymphocyte alloantigen not coded for by the MHC

Equine lymphocyte antigens in four major Belgian horse populations. Contribution to serology and antigen distribution

Immunological aspects of pregnancy in mules

Contact Info

Product

Resources

About