Joint Healthcare Infection Society (HIS) and Infection Prevention Society (IPS) guidelines for the prevention and control of meticillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities

Coia, John; Wilson, Jennie; Bak, Aggie; Marsden, Gemma; Shimonovich, Michal; Loveday, Heather; Humphreys, Hilary; Wigglesworth, Neil; Demirjian, Alicia; Brooks, Julie; Butcher, Lisa; Price, James R.; Ritchie, Lauren E.; Newsholme, William; Enoch, David; Bostock, Julieanne M.; Cann, M.; Wilson, A.P.R.

doi:10.1016/j.jhin.2021.09.022

Cited by 17 publications

(9 citation statements)

References 253 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Egypt, 5.5–8.8% of S. aureus isolates were VRSA ( Amr and Gammal 2017 ; ElSayed et al 2018 ). The determination of S. aureus resistance is crucial for optimal antimicrobial therapy, as well as for epidemiological knowledge and effective infection control strategies ( Prestinaci et al 2015 ; Coia et al 2021 ). Since 2008, Lithuania has applied a national prevention program to control the spread of antimicrobial resistance ( Ministry of Health of The Republic of Lithuania 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and the Prevalence of the Panton-Valentine Leukocidin Gene among Clinical Isolates of Staphylococcus aureus in Lithuania

Kirkliauskienė,

Kriščiūnas,

Miciulevičienė

et al. 2024

Polish Journal of Microbiology

View full text Add to dashboard Cite

This study aimed to determine resistance to antimicrobials of Staphylococcus aureus strains isolated from clinical specimens in Lithuanian hospitals and to identify the genes conferring resistance and virulence. The study was carried out from June 2019 to September 2021. S. aureus strains were isolated from skin, soft tissues, blood, lower respiratory tract, urine and other specimens. Antibiotic susceptibility testing was performed using the disc diffusion method according to EUCAST guidelines. All isolates were analyzed for detection of the ermA, ermC, mecA, mecC, tetK, tetM, and lukF-PV genes by multiplex real-time PCR. The 16S rRNA coding sequence was applied as an internal PCR control. Altogether, 745 S. aureus strains were analyzed. Antimicrobial susceptibility testing revealed that all isolates were susceptible to rifampin and vancomycin. Of the 745 strains, 94.8% were susceptible to tetracycline, 94.5% to clindamycin, and 88.3% to erythromycin. The lowest susceptibility rate was found for penicillin (25.8%). Six percent of the tested strains were methicillin-resistant S. aureus (MRSA). The majority of methicillin-resistant strains were isolated from skin and soft tissues (73.3%), with a smaller portion isolated from blood (17.8%) and respiratory tract (8.9%). The ermC gene was detected in 41.1% of erythromycin-resistant S. aureus strains, whereas ermA was detected in 32.2% of erythromycin-resistant S. aureus strains. 69.2% of tetracycline-resistant S. aureus strains had tetK gene, and 28.2% had tetM gene. 7.3% of S. aureus isolates harbored lukF-PV gene. The frequency of the pvl gene detection was significantly higher in MRSA isolates than in methicillin-susceptible S. aureus isolates (p < 0.0001).

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and the Prevalence of the Panton-Valentine Leukocidin Gene among Clinical Isolates of Staphylococcus aureus in Lithuania

Kirkliauskienė,

Kriščiūnas,

Miciulevičienė

et al. 2024

Polish Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…In Egypt, 5.5-8.8% of S. aureus isolates were VRSA [26,27]. The determination of S. aureus resistance is crucial for optimal antimicrobial therapy, as well as for epidemiological knowledge and effective infection control strategies [28,29].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance and the prevalence of the Panton-Valentine Leukocidin gene among clinical isolates of Staphylococcus aureus in Lithuania

Kirkliauskienė,

Kriščiūnas,

Miciulevičienė

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Staphylococcus aureus is one of the most frequently identified pathogens causing nosocomial infections worldwide and, in many cases, exhibits resistance to multiple antimicrobials. However, there is a lack of data about the prevalence of S. aureus resistance to antimicrobials in major hospitals in Vilnius city, Lithuania. Therefore, the aim of this study was to determine the resistance to antimicrobials of Staphylococcus aureus strains isolated from clinical specimens in Lithuanian hospitals and to identify the genes conferring resistance and virulence. Methods This study was carried out from June 2019 to September 2021. S. aureus strains were isolated from clinical specimens. Antibiotic susceptibility testing was performed by the disc diffusion method according to EUCAST guidelines. All our collected isolates were analysed for detection of the ermA, ermC, mecA, mecC, tetK, tetM and lukF-PV genes by multiplex real-time PCR. The 16S rRNA coding sequence was applied as an internal PCR control. Results A total of 745 S. aureus strains isolated from clinical specimens were analysed. A total of 69.0% of strains were isolated from the skin and soft tissues, 14.5% from blood, 11.7% from the lower respiratory tract, 2.7% from urine and 2.1% from other specimens. Antimicrobial susceptibility testing revealed that all 745 isolates were susceptible to rifampin and vancomycin. Of the 745 strains, 94.8% were susceptible to tetracycline, 94.5% to clindamycin and 88.3% to erythromycin. The lowest susceptibility rate was found to penicillin (25.8%). Six percent of the tested strains were methicillin-resistant S. aureus (MRSA). The majority of methicillin-resistant strains were isolated from skin and soft tissues (73.3%), with a smaller portion isolated from blood (17.8%) and the respiratory tract (8.9%). The ermC gene was detected in 41.1% of erythromycin-resistant S. aureus strains, whereas ermA was detected in 32.2% of erythromycin-resistant S. aureus strains. A total of 69.2% of tetracycline-resistant S. aureus strains had the tetK gene, and 28.2% had the tetM gene. A total of 7.3% of S. aureus isolates harboured the lukF-PV gene. The frequency of pvl gene detection was significantly higher in the MRSA isolates than in the methicillin-susceptible S. aureus (MSSA) isolates (p < 0.0001). Conclusions All tested strains were susceptible to rifampicin and vancomycin. A total of 6% of the strains were MRSA. The lowest susceptibility rate was found to penicillin. The tetK gene was predominant among S. aureus strains resistant to tetracycline, and the ermC gene was predominant among erythromycin-resistant strains. Seven percent of S. aureus isolates harboured the lukF-PV gene, which was more prevalent in methicillin-resistant strains.

show abstract

“…14 Recently updated National Institute of Clinical Excellence (NICE) guidelines on the management of MRSA have removed a recommendation specifically listing umbilicus and throat as preferential screening sites in neonates, citing a lack of evidence. 15 …”

Section: Challenges With Interventions In Neonatesmentioning

confidence: 99%

Staphylococcal aureus outbreaks in neonatal intensive care units: strategies, nuances, and lessons learned from the frontline

Rooney,

Lancaster,

McKechnie

et al. 2024

ASHE

View full text Add to dashboard Cite

Methicillin-resistant Staphylococcus aureus (MRSA) infections in neonates can result in significant morbidity and mortality. However, comparatively to adults, neonatal MRSA data remains relatively scarce. Additionally, while evidence-driven practices for adults have seen considerable progress, neonatal infection prevention strategies remain poorly described. The Leeds Newborn Service adopted a series of infection prevention and control (IPC) measures following a rise in MRSA cases in 2008–2009. This narrative review presents IPC measures for neonatal MRSA and methicillin-sensitive Staphylococcus aureus (MSSA) infections and reflects upon local challenges and successes of these interventions. Our experience underscores the importance of an adaptive, evidence-based strategy, tailored to the neonatal population. Effectively addressing MRSA/MSSA requires continuous monitoring with sustained targeted interventions. Our key learning points highlight the intertwined difficulties of specific neonatal requirements and lack of definitive IPC guidance, suggesting a holistic approach is key for successful IPC outcomes in the neonatal intensive care unit setting.

show abstract

Joint Healthcare Infection Society (HIS) and Infection Prevention Society (IPS) guidelines for the prevention and control of meticillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities

Cited by 17 publications

References 253 publications

Antimicrobial Resistance and the Prevalence of the Panton-Valentine Leukocidin Gene among Clinical Isolates of Staphylococcus aureus in Lithuania

Antimicrobial Resistance and the Prevalence of the Panton-Valentine Leukocidin Gene among Clinical Isolates of Staphylococcus aureus in Lithuania

Antimicrobial resistance and the prevalence of the Panton-Valentine Leukocidin gene among clinical isolates of Staphylococcus aureus in Lithuania

Staphylococcal aureus outbreaks in neonatal intensive care units: strategies, nuances, and lessons learned from the frontline

Contact Info

Product

Resources

About