Joint distraction attenuates osteoarthritis by reducing secondary inflammation, cartilage degeneration and subchondral bone aberrant change

Chen, Y.; Sun, Yuxin; Xiao-ting, Pan; Ho, Kwok W.; Li, Gang

doi:10.1016/j.joca.2015.05.018

Cited by 50 publications

(71 citation statements)

References 59 publications

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“…That might be the reason for more cartilage still remaining in the PBS group and medium group in our current study. Moreover, once committed to the osteogenic lineage, MSCs express Osx and OCN which are markers of osteoprogenitors [24, 38]. The number of Osx- and OCN-positive osteoprogenitors was significantly upregulated in the distraction zone after treatment with hFMSC secretome compared to the control treatment groups.…”

Section: Discussionmentioning

confidence: 99%

“…Immunohistochemistry staining was performed using a standard protocol as previously reported [24]. Secretions were treated with primary antibodies to rabbit osterix (Osx; Abcam, 1:100, ab22552) and osteocalcin (OCN; Santa Cruz, 1:100, sc30045) overnight at 4 °C; a horseradish peroxidase-streptavidin detection system (Dako, USA) was used, followed by counterstaining with hematoxylin.…”

Section: Methodsmentioning

confidence: 99%

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Human fetal mesenchymal stem cell secretome enhances bone consolidation in distraction osteogenesis

Wang²,

Sun

et al. 2016

Stem Cell Res Ther

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BackgroundDistraction osteogenesis (DO) is one of the most dramatic reconstructive techniques for inducing bone regeneration, but it involves an undesirably long period for bone consolidation. Developing innovative approaches to enhance bone consolidation is a burning need. Human fetal mesenchymal stem cells (hFMSCs) have been shown to express more primitive developmental genes than those of human adult mesenchymal stem cells (hAMSCs), which is a preferable source for cell therapy and tissue regeneration. In the present study, we investigated the immunogenicity of using the human mesenchymal stem cell (MSC) secretome on rat cells, the effects of secretome on osteogenic differentiation of rat bone marrow-derived MSCs (rBMSCs), and the potential application of hFMSC secretome in promoting bone consolidation in a rat DO model.MethodsSecretome was collected from MSC culture and was used to treat rBMSCs. Following secretome treatment, cell proliferation, alkaline phosphatase staining, Alizarin Red S staining, and mRNA expression of osteogenic differentiation-related genes (including ALP, Runx2, OCN, OPN, and Osx) in the rBMSCs were checked, as well as mixed rat peripheral blood lymphocyte reaction. hFMSC secretome was injected locally into the regenerates from the end of lengthening every 3 days in the rat DO model, until termination. The regenerates were subject to weekly x-rays, micro-computed tomography (μCT) and mechanical testing examination. The bone quality was assessed by histology and immunohistochemistry examinations.ResultsCompared to the secretome from rBMSCs and hAMSCs, hFMSC secretome had the best osteogenic induction ability and low immunogenicity. hFMSC secretome with different doses showed no effect on cell viability. hFMSC secretome at the dose of 100 μg/μl could significantly increase the expression of alkaline phosphatase and all the osteogenic marker genes, as well as the amount of calcium deposits in the rBMSCs. Finally, the local application of hFMSC secretome in distraction regenerates in a rat DO model significantly improved bone consolidation according to the results of μCT, mechanical test, and histological and immunohistochemistry analysis.ConclusionsThe current study demonstrated that hFMSC secretome promotes osteogenesis of rBMSCs and bone consolidation during DO. hFMSC secretome may be a new therapeutic strategy to enhance bone consolidation in patients undergoing DO treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Human fetal mesenchymal stem cell secretome enhances bone consolidation in distraction osteogenesis

Wang²,

Sun

et al. 2016

Stem Cell Res Ther

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“…O processo patológico da doença ativa afeta todos os tecidos da articulação, levando a uma alteração metabólica local, alterando o processo de reparo tecidual e culminando com uma insuficiência articular (SHARMA;ELISSEEFF, 2004;ARLIANI et al, 2014;CHEN et al, 2015).…”

Section: Populações De Ct Foram Isoladas Também Da Polpa Dentária Humunclassified

“…Lesões osteocondrais degenerativas e progressivas são caracterizadas por alterações na cartilagem articular e tecido ósseo, sendo a articulação do joelho a mais afetada (BENNELL; HINMAN, 2011;ROI et al, 2015;CHEN et al, 2015). Um desses processos é conhecido como osteoartrite (OA), um processo inflamatório degenerativo que acomete a articulação e o osso subcondral (SCHMINKE;MIOSGE, 2014;CHEN et al, 2015).…”

Section: Introductionunclassified

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Reparo de lesões induzidas na articulação femorotibiopatelar de ovinos através do implante de biomateriais associados à células-tronco de polpa dentária humana

Silva¹

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Staphylococcal enterotoxin C2 expedites bone consolidation in distraction osteogenesis

Sun

et al. 2016

J. Orthop. Res.

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Distraction osteogenesis (DO) technique could be used to manage large-size bone defect successfully, but DO process usually requires long duration of bone consolidation. Innovative approaches for augmenting bone consolidation are of great need. Staphylococcal enterotoxin C2 (SEC2) has been found to suppress osteoclastogenesis of mesenchymal stem cells in vitro. In this study, we investigated the effect of SEC2 on proliferation and osteogenic differentiation of rat bone marrow derived mesenchymal stem cells (rBMSCs). Further, we locally administrated SEC2 (10 ng/ml) or PBS into the distraction gap in Sprague-Dawley male rat DO model every 3 days till termination at 3 and 6 weeks. The regenerates were subjected to X-rays, micro-computed tomography, mechanical testing, histology, and immunohischemistry examinations to assess new bone quality. SEC2 had no effect on cell viability. The calcium deposition was remarkably increased and osteogenic marker genes were significantly up-regulated in rBMSCs treated with SEC2. In rat DO model, SEC2 group had higher bone volume/total tissue volume in the regenerates. At 6 weeks, mechanical properties were significantly higher in SEC2-treated tibiae comparing to the control group. Histological analysis confirmed that the new bone had improved quality in SEC2 treated group, where the osteocalcin and osterix expression in the regenerates was up-regulated, indicating faster bone formation. The current study demonstrated that SEC2 local injection promotes osteogenesis and enhanced bone consolidation in DO. The findings support application of SEC2 as a potential novel strategy to expedite bone consolidation in patients undergoing DO treatment. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1215-1225, 2017.

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Joint distraction attenuates osteoarthritis by reducing secondary inflammation, cartilage degeneration and subchondral bone aberrant change

Abstract: In the present study, we demonstrated that joint distraction reduced the level of secondary inflammation, cartilage degeneration and subchondral bone aberrant change, joint distraction may be a strategy for slowing OA progression.

Cited by 50 publications

References 59 publications

Human fetal mesenchymal stem cell secretome enhances bone consolidation in distraction osteogenesis

Human fetal mesenchymal stem cell secretome enhances bone consolidation in distraction osteogenesis

Reparo de lesões induzidas na articulação femorotibiopatelar de ovinos através do implante de biomateriais associados à células-tronco de polpa dentária humana

Staphylococcal enterotoxin C2 expedites bone consolidation in distraction osteogenesis

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