Joint analysis of left ventricular expression and circulating plasma levels of Omentin after myocardial ischemia

Abstract

BackgroundOmentin-1, also known as Intelectin-1 (ITLN1), is an adipokine with plasma levels associated with diabetes, obesity, and coronary artery disease. Recent studies suggest that ITLN1 can mitigate myocardial ischemic injury but the expression of ITLN1 in the heart itself has not been well characterized. The purpose of this study is to discern the relationship between the expression pattern of ITLN1 RNA in the human heart and the level of circulating ITLN1 protein in plasma from the same patients following myocardial ischemia.MethodsA large cohort of patients (n = 140) undergoing elective cardiac surgery for aortic valve replacement were enrolled in this study. Plasma and left ventricular biopsy samples were taken at the beginning of cardiopulmonary bypass and after an average of 82 min of ischemic cross clamp time. The localization of ITLN1 in epicardial adipose tissue (EAT) was also further characterized with immunoassays and cell fate transition studies.ResultsmRNA expression of ITLN1 decreases in left ventricular tissue after acute ischemia in human patients (mean difference 280.48, p = 0.001) whereas plasma protein levels of ITLN1 increase (mean difference 5.24, p < 0.001). Immunohistochemistry localized ITLN1 to the mesothelium or visceral pericardium of EAT. Epithelial to mesenchymal transition in mesothelial cells leads to a downregulation of ITLN1 expression.ConclusionsMyocardial injury leads to a decrease in ITLN1 expression in the heart and a corresponding increase in plasma levels. These changes may in part be due to an epithelial to mesenchymal transition of the cells that express ITLN1 following ischemia. Trial Registration Clinicaltrials.gov ID: NCT00985049Electronic supplementary materialThe online version of this article (doi:10.1186/s12933-017-0567-x) contains supplementary material, which is available to authorized users.

Plasma omentin levels are inversely associated with atherosclerosis in type 2 diabetes patients with increased plasma adiponectin levels: a cross-sectional study

“…While studies in individuals without CVDs indicated an inverse relationship between omentin and subclinical atherosclerosis [6, 11, 13, 14], studies in patients with established CVDs demonstrated that higher omentin levels were cross-sectionally associated with the severity of coronary artery disease [35, 39] and were longitudinally associated with adverse CVD outcome [40, 41]. Increased plasma omentin levels were also demonstrated after acute cardiac ischemia in patients undergoing cardiac surgery [42]. Importantly, in patients with coronary artery disease and ≥ 90% coronary occlusion, plasma omentin levels were not only increased with the disease severity, but also were independently associated with better collateral circulation, or better endothelial function [39].…”

Association between plasma omentin-1 levels in type 2 diabetic patients and peripheral artery disease

“…Other studies have investigated the relationship between omentin-1 and coronary artery disease [14,15], stroke, and complications of T2DM [13,16]. Based on these findings, some controversial data emerged about a negative, positive, or independent correlation between omentin-1 levels and cardiovascular risk factors.…”

Cardioprotective Effect of Danshensu against Ischemic/Reperfusion Injury via c‐Subunit of ATP Synthase Inhibition

“…Cardiomyocytes are sensitive to the changes of microenvironment, especially ischemia. Numerous studies have reported that mRNA transcription and protein expression change during the first 90 minutes of ischemic-reperfusion episode in both human and animals [ 31 , 32 ]. ATP 5G1, which target to mitochondria, can be imported into mitochondria under ischemic conditions [ 10 , 29 ].…”