JNK signaling in pioneer neurons organizes ventral nerve cord architecture in Drosophila embryos

Karkali, Katerina; Saunders, Timothy E.; Panayotou, George; Martı́n-Blanco, Enrique

doi:10.1038/s41467-023-36388-1

Cited by 5 publications

(17 citation statements)

References 61 publications

(81 reference statements)

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“…MARCM clones of this allele induced in the embryo engender a mutant phenotype. The phenotype ( n = 6) is a ventral cord that fails to condense, a phenotype recently reported for JNK signaling ( Karkali et al . 2023 ).…”

Section: Resultsmentioning

confidence: 70%

Fourth Chromosome Resource Project: a comprehensive resource for genetic analysis in Drosophila that includes humanized stocks

Stinchfield,

Weasner,

Weasner

et al. 2023

Genetics

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The fourth chromosome is the final frontier for genetic analysis in Drosophila. Small, heterochromatic, and devoid of recombination the fourth has long been ignored. Nevertheless, its long arm contains 79 protein-coding genes. The Fourth Chromosome Resource Project (FCRP) has a goal of facilitating the investigation of genes on this neglected chromosome. The project has 446 stocks publicly available at the Bloomington and Kyoto stock centers with phenotypic data curated by the FlyBase and FlyPush resources. Four of the five stock sets are nearly complete: (1) UAS.fly cDNAs, (2) UAS.human homolog cDNAs, (3) gene trap mutants and protein traps, and (4) stocks promoting meiotic and mitotic recombination on the fourth. Ongoing is mutagenesis of each fourth gene on a new FRT-bearing chromosome for marked single-cell clones. Beyond flies, FCRP facilitates the creation and analysis of humanized fly stocks. These provide opportunities to apply Drosophila genetics to the analysis of human gene interaction and function. In addition, the FCRP provides investigators with confidence through stock validation and an incentive via phenotyping to tackle genes on the fourth that have never been studied. Taken together, FCRP stocks will facilitate all manner of genetic and molecular studies. The resource is readily available to researchers to enhance our understanding of metazoan biology, including conserved molecular mechanisms underlying health and disease.

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Section: Resultsmentioning

confidence: 70%

Fourth Chromosome Resource Project: a comprehensive resource for genetic analysis in Drosophila that includes humanized stocks

Stinchfield,

Weasner,

Weasner

et al. 2023

Genetics

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“…Pioneer neurons and tracts were discovered in seminal studies in Drosophila (Harrison, 1959) and recent studies show to be required for other neuronal cell body correct positioning (Karkali et al, 2023). In zebrafish, a group of pioneer neurons is required for correct migration of later facial branchiomotor neurons (Wanner & Prince, 2013).…”

Section: The Posterior Placodal Area Providing Pioneer Neurons As Org...mentioning

confidence: 99%

“…Specially studied in invertebrates is the modulation of neuronal dynamics during development by pioneer cells. These pioneer cells are defined as early born cells that have a scaffolding capacity to organize the tissue and preform the final configuration (Aigouy et al, 2008;Karkali et al, 2023;Wanner & Prince, 2013;Whitlock & Westerfield, 1998).…”

Section: Introductionmentioning

confidence: 99%

Role of pioneer neurons and neuroblast behaviors on otic ganglion assembly

Bañón

Alsina

2023

Preprint

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Cranial ganglia are aggregates of sensory neurons that mediate distinct types of sensation. It is little understood how individual neurons coalesce, distribute and shape the ganglion. The statoacoustic ganglion (SAG) displays several lobes spatially arranged to properly connect with hair cells of the inner ear. To investigate the cellular behaviors involved in the 3D organization of the SAG, we use high resolution confocal imaging of single cell labeled zebrafish neuroblasts (NB), photoconversion, photoablation and genetic perturbations. We find that otic NB delaminate out of the otic epithelium in an EMT-like manner, rearranging apical polarity and primary cilia proteins. We also show that, once delaminated, NB migrate directionally and actively, requiring RhoGTPases. Interestingly, cell tracking of individual delaminated NB reveals that NB migrate and coalesce around a small population of pioneer SAG neurons. These pioneer SAG neurons are not from otic placode origin and populate the coalescence region before otic neurogenesis begins. Upon ablation of these cells, migratory pathways of delaminated NB are disrupted and, consequently, SAG shape is affected. Altogether, this work shows for the first time the role of pioneer SAG neurons in orchestrating SAG development.

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“…Pioneer neurons and tracts were discovered in seminal studies in Drosophila (Harrison, 1959) and recent studies show that they are required for other neuronal body positioning (Karkali et al, 2023). In zebrafish, a group of pioneer neurons is required for correct migration of later facial branchiomotor neurons (Wanner and Prince, 2013).…”

Section: Nb Otic Delamination As An Emt Processmentioning

confidence: 99%

“…One such behaviour is the modulation of neuronal dynamics by pioneer cells. These pioneer cells, first described in invertebrates, are defined as early-born cells that have a scaffolding capacity to organize the tissue and pre-form the final configuration (Aigouy et al, 2008;Karkali et al, 2023;Wanner and Prince, 2013;Whitlock and Westerfield, 1998). However, whether other cells also participate in SAG formation is unknown.…”

Section: Introductionmentioning

confidence: 99%

Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly

Bañón,

Alsina

2023

Development

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Cranial ganglia are aggregates of sensory neurons that mediate distinct types of sensation. The statoacoustic ganglion (SAG) develops into several lobes that are spatially arranged to connect appropriately with hair cells of the inner ear. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion, photoablation, and genetic perturbations. We show that otic NBs delaminate out of the otic epithelium in an epithelial-mesenchymal transition-like manner, rearranging apical polarity and primary cilia proteins. We also show that, once delaminated, NBs require RhoGTPases in order to perform active migration. Furthermore, tracking of recently delaminated NBs revealed their directed migration and coalescence around a small population of pioneer SAG neurons. These pioneer SAG neurons, not from otic placode origin, populate the coalescence region before otic neurogenesis begins and their ablation disrupts delaminated NB migratory pathways, consequentially affecting SAG shape. Altogether, this work shows for the first time the role of pioneer SAG neurons in orchestrating SAG development.

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JNK signaling in pioneer neurons organizes ventral nerve cord architecture in Drosophila embryos

Cited by 5 publications

References 61 publications

Fourth Chromosome Resource Project: a comprehensive resource for genetic analysis in Drosophila that includes humanized stocks

Fourth Chromosome Resource Project: a comprehensive resource for genetic analysis in Drosophila that includes humanized stocks

Role of pioneer neurons and neuroblast behaviors on otic ganglion assembly

Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly

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