JMM Profile: Vibrio cholerae: an opportunist of human crises

Grant, Trudy-Ann; Balasubramanian, Deepak; Almagro‐Moreno, Salvador

doi:10.1099/jmm.0.001423

Cited by 8 publications

(4 citation statements)

References 10 publications

(18 reference statements)

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“…Since the publication of our first Pathogen Profile on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by Professor Tim Inglis (JMM Deputy Editor-in-Chief) and Professor Kalai Mathee (JMM Co-Editor-in-Chief) [2], we have published a number of profiles, including those on Vibrio cholerae , Bordetella pertussis and Streptococcus pneumoniae [3–5]. We have also published our first Antimicrobial Profile on carbapenems [6], while our first Diagnostic Profile on loop-mediated isothermal amplification (LAMP) is forthcoming.…”

Section: Full-textmentioning

confidence: 99%

JMM Profiles for the Journal of Medical Microbiology: an update

Fry¹,

Ragione

2022

Journal of Medical Microbiology

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Section: Full-textmentioning

confidence: 99%

JMM Profiles for the Journal of Medical Microbiology: an update

Fry¹,

Ragione

2022

Journal of Medical Microbiology

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“…Vibrio cholerae , a Gram-negative, curved rod, comma-shaped bacterium, the causative agent of cholera, is a significant global public health concern, particularly in the regions with limited access to clean water and sanitation ( 1 ). V. cholerae is responsible for severe diarrheal outbreaks, leading to high morbidity and mortality rates worldwide.…”

Section: Introdutionmentioning

confidence: 99%

The Role of Morphological Adaptability inVibrio cholerae’s Motility and Pathogenicity

Xu,

Abe,

Kodama

et al. 2024

Preprint

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Vibrio cholerae, the etiological agent of cholera, exhibits remarkable adaptability to different environmental conditions by undergoing morphological changes that significantly contribute to its pathogenicity and impact the epidemiology of the disease globally. This study investigates the morphological adaptability of the clinically isolatedV. choleraeO1 strain, specifically focusing on the motility and pathogenicity differences between the filamentous and original comma-shaped forms within diverse viscosity conditions. Utilizing the El Tor strain ofV. choleraeO1, we induced the transformation into the filamentous form and performed a comparative analysis with the canonical comma-shaped morphology. Our approach involved assessing motility patterns, swimming speeds, rotation rates, kinematics, and reversal frequencies through dark-field microscopy and high-speed imaging techniques. The findings reveal that filamentousV. choleraecell retains enhanced motility in viscous environments. This suggests an evolutionary adaptation enabling survival across a range of habitats, notably the human gastrointestinal tract. Filamentous forms demonstrated increased reversal behavior at mucin interfaces, hinting at an advantage in penetrating the mucus layer. Rabbit intestinal loop assays further showed that both morphological forms exhibit similar fluid accumulation ratios, thus indicating comparable pathogenic potentials. These results underscore the significance ofV. cholerae's morphological flexibility in adapting to environmental viscosity changes, shedding light on the bacterium's intricate survival and infection strategies. Our study provides critical insights into the dynamics of cholera, underlining the importance of considering bacterial morphology in developing effective cholera control strategies.

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“…1 The current cholera pandemic is responsible for over 140.000 deaths annually in communities and countries where proper water sanitation is limited, especially in the wake of natural disasters and armed conflicts. 2 The Gram-negative bacterium Vibrio cholerae is the pathogenic agent responsible for cholera.…”

Section: Introductionmentioning

confidence: 99%

Calcium binding site in AA10 LPMO fromVibrio choleraesuggests modulating effects during environment survival and infection

Montserrat-Canals,

Bjerregard-Andersen,

Sørensen

et al. 2023

Preprint

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Despite major efforts towards its eradication, cholera remains a major health and economic burden in many developing countries. Between outbreaks, the bacterium responsible for the disease,Vibrio cholerae, survives in aquatic environmental reservoirs, where it commonly forms biofilms,e.g., on zooplankton.N-acetyl glucosamine binding protein A (GbpA) is an adhesin that binds to the chitinaceous surface of zooplankton and breaks its dense crystalline packing thanks to its lytic polysaccharide monooxygenase (LPMO) activity, which providesV. choleraewith nutrients. In addition, GbpA is an important colonization factor associated with bacterial pathogenicity, allowing the binding to mucins in the host intestine. Here, we report the discovery of a cation-binding site in proximity of the GbpA active site, which allows Ca2+, Mg2+or K+to bind close to its carbohydrate-binding surface. In addition to the X-ray crystal structures, we explored how the presence of ions affects the stability of the protein, compared the new GbpA LPMO structures to those of other LPMOs, and discussed the relevance of our discovery for bacterial survival. Calcium ions, abundant in natural sources of chitin, have been found to have the strongest effect on GbpA stability. Our findings suggest aV. cholerae-specific cation-binding site in GbpA that may fine-tune activity and binding to the different substrates during environmental survival and host infection.

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JMM Profile: Vibrio cholerae: an opportunist of human crises

Cited by 8 publications

References 10 publications

JMM Profiles for the Journal of Medical Microbiology: an update

JMM Profiles for the Journal of Medical Microbiology: an update

The Role of Morphological Adaptability inVibrio cholerae’s Motility and Pathogenicity

Calcium binding site in AA10 LPMO fromVibrio choleraesuggests modulating effects during environment survival and infection

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