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Abstract

The biocompatibility of material plays an important role in the bone-implant interface for the prosthetic implant fixation. The biocompatibility of implants is associated with the chemical composition, surface topography, surface energy and surface roughness of biomaterials. The effects of two factors, surface roughness and serum contents, on osteoblast behavior at the surface of Ti-6Al-4V and plasma sprayed HA coating were investigated in the experiment. The osteoblasts derived from neonatal rat calvarial were cultured in Dulbecco's modified Eagle medium (DMEM) with fetal bovine serum (FBS) on the surface of polished Ti-6Al-4V (Ti-p), grit-blasted Ti-6Al-4V (Ti-b), polished HA coating (HAC-p), and as-sprayed HA coating (HAC). Under culture medium containing 4% FBS, the level of cell attachment to the polished surface is significantly higher than the rough surface of the same experimental materials during all culture periods. Increasing the contents of FBS up to 10%, the difference of osteoblast attachment is not found between Ti-p and Ti-b. Under 4% serum condition, the cell morphology attached to smooth surfaces (Ti-p and HAC-p) is spread faster and are more flattened than the one to rough surface of the same experimental materials by SEM. After 24 h culture, the corroded cracks are easily observed at the surface of polished HA coatings, and the cell morphology on HAC-p coatings are elongated and less flattened compared with Ti-p. The result is consistent with statistical difference of cell attachment between Ti-p and HAC-p under 4% serum condition.

Biomechanical Properties and Corrosion Resistance of Plasma-Sprayed Fish Scale Hydroxyapatite (FsHA) and FsHA-Doped Yttria-Stabilized Zirconia Coatings on Ti–6Al–4V Alloy for Biomedical Applications

“…The very high-intensity HA peak observed in the spectrum could be attributed to the optimum coating parameters and fine powder particle size used in this work. The main phases observed in the coating were HA, CaO, TTCP, and β-TCP, which agreed with reports that plasma-sprayed HA coatings usually contain α and β-TCP, TTCP, CaO, and an amorphous calcium phosphate mixture in addition to crystalline HA [42]. Figure 8b-d also shows the XRD patterns of the plasma-sprayed FsHA coatings with 10, 15, and 20 wt.% YSZ, with the different phases identified in the coatings labelled accordingly.…”

“…HA [42]. Figure 8b-d also shows the XRD patterns of the plasma-sprayed FsHA coatings with 10, 15, and 20 wt.% YSZ, with the different phases identified in the coatings labelled accordingly.…”

“…Surface roughness is described by many researchers as vital for implant-tissue interactions in clinical use. Clinically used implants are characterized by micro-pitted surfaces made by either plasma spraying, sand blasting, acid etching or laser treatment [42,43]. Table 3 lists the surface roughness values (Ra, Rz and Rmax) of the plasma-sprayed FsHA/YSZ coatings.…”

Hydroxyapatite Film Coating by Er:YAG Pulsed Laser Deposition Method for the Repair of Enamel Defects

“…The HAp film coating on the etched bovine enamel was sterilized by autoclaving (121 °C, 125 kPa, 60 min) and furbished [ 24 ] (silicon carbide abrasive paper until #4000) for cell culture [ 25 ]. For SEM analysis, the HPdLF cells were processed for fixation and then gently rinsed three times with phosphate-buffered saline (PBS).…”