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Bell, Kenneth S.; Roberts, Jacqueline; Verrall, Susan; Cullen, Daniel W.; Williams, N. A.; Harrison, J. G.; Toth, Ian K.; Cooke, D. E. L.; Duncan, James M.; Claxton, J. R.

doi:10.1023/a:1008782309333

Cited by 46 publications

(2 citation statements)

References 27 publications

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“…Molecular methods based on PCR (Bulman and Marshall 1998) can also be used for highly sensitive laboratory-based Sss detection. Bell et al (1999) used primers similar to those reported by Bulman and Marshall (1998), and detected amplification from peel and washings of apparently healthy tubers. Using real-time PCR, van de Graaf et al (2005) found that symptomless infection was common for tubers harvested from their pot trials, especially under conditions suboptimal for powdery scab development.…”

Section: Seed Tuber Infectionmentioning

confidence: 81%

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Review: Powdery Scab of Potato—Increased Knowledge of Pathogen Biology and Disease Epidemiology for Effective Disease Management

Merz

Falloon

2008

Potato Res.

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Section: Seed Tuber Infectionmentioning

confidence: 81%

“…Bell et al (1999) detected as few as one to ten sporosori/g soil applying an adapted DNA extraction method. The biggest problem to overcome was with humic acids which interfered with the PCR.…”

Section: Soil Inoculum Detection and Quantificationmentioning

confidence: 99%

Review: Powdery Scab of Potato—Increased Knowledge of Pathogen Biology and Disease Epidemiology for Effective Disease Management

Merz

Falloon

2008

Potato Res.

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Detection and Identification of Fungal Pathogens

2017

Microbial Plant Pathogens

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Molecular Techniques for Detection of Microbial Pathogens

Narayanasamy

Molecular Biology in Plant Pathogenesis and Disease Management

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Detection, identification and differentiation of microbial plant pathogens -oomycetes, fungi, bacteria, phytoplasmas, viruses and viroids -infecting various crops constitute the basic step for the development of effective crop disease management systems. The conventional cultural methods involving isolation and studying the morphological characteristics using microscope are labor intensive and cumbersome, yielding sometimes, inconclusive results. The molecular techniques, on the other hand, are able to provide precise, reliable and reproducible results rapidly, facilitating early disease management decisions. Biochemical, immunological and nucleic acid-based assays have been preferred, because of the distinct advantages over the conventional methods. The molecular techniques have been very useful in the identification of obligate pathogens causing diseases such as downy mildews, powdery mildews and rusts and also fungi that grow very slowly in the culture media, taking several weeks to produce spore forms that can be used for identification. The availability of antisera, primers and commercial kits has led to widespread application of the molecular techniques for on-site detection during field surveys for assessing the distribution of existing pathogens, occurrence of new/introduced pathogens or strains and for preventing introduction of new pathogens through seeds or planting materials. Furthermore, molecular techniques have been demonstrated to be useful in breeding programs to identify sources of resistance to disease(s). Several protocols for molecular techniques, useful for researchers and students are presented in the Appendix.

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Cited by 46 publications

References 27 publications

Review: Powdery Scab of Potato—Increased Knowledge of Pathogen Biology and Disease Epidemiology for Effective Disease Management

Review: Powdery Scab of Potato—Increased Knowledge of Pathogen Biology and Disease Epidemiology for Effective Disease Management

Detection and Identification of Fungal Pathogens

Molecular Techniques for Detection of Microbial Pathogens

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