JIP1 Mediates Anterograde Transport of Rab10 Cargos during Neuronal Polarization

Deng, Cai-Yun; Lei, Wenliang; Xu, Xiaohui; Ju, Xiang-Chun; Liu, Yang; Luo, Zhen-Ge

doi:10.1523/jneurosci.4496-13.2014

Cited by 50 publications

(51 citation statements)

References 59 publications

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“…It is highly expressed in neurons and through interplay with AKT, promotes axonal growth in cortical cultures [33,34]. In vivo, it is important for polarization and migration of cortical neurons [35]. Serine/threonine-protein kinase MARK1 (microtubule affinity-regulating kinase 1) encodes a kinase-regulating microtubule-dependent transport in axons and dendrites [36].…”

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis of Mouse Cortex Postsynaptic Density following Neonatal Brain Hypoxia-Ischemia

Shao

Wang²,

Guan³

et al. 2017

Dev Neurosci

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Proteomics of the synapses and postsynaptic densities (PSDs) have provided a deep understanding of protein composition and signal networks in the adult brain, which underlie neuronal plasticity and neurodegenerative or psychiatric disorders. However, there is a paucity of knowledge about the architecture and organization of PSDs in the immature brain, and how it is modified by brain injury in an early developing stage. Mass spectrometry (MS)-based proteomic analysis was performed on PSDs prepared from cortices of postnatal day 9 naïve mice or pups which had suffered hypoxic-ischemic (HI) brain injury. 512 proteins of different functional groups were identified from PSDs collected 1 h after HI injury, among which 60 have not been reported previously. Seven newly identified proteins involved in neural development were highlighted. HI injury increased the yield of PSDs at early time points upon reperfusion, and multiple proteins were recruited into PSDs following the insult. Quantitative analysis was performed using spectral counting, and proteins whose relative expression was more than 50% up- or downregulated compared to the sham animals 1 h after HI insult were reported. Validation with Western blotting demonstrated changes in expression and phosphorylation of the N-methyl-D-aspartate receptor, activation of a series of postsynaptic protein kinases and dysregulation of scaffold and adaptor proteins in response to neonatal HI insult. This work, along with other recent studies of synaptic protein profiling in the immature brain, builds a foundation for future investigation on the molecular mechanisms underlying developing plasticity. Furthermore, it provides insights into the biochemical changes of PSDs following early brain hypoxia-ischemia, which is helpful for understanding not only the injury mechanisms, but also the process of repair or replenishment of neuronal circuits during recovery from brain damage.

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Section: Resultsmentioning

confidence: 99%

Proteomic Analysis of Mouse Cortex Postsynaptic Density following Neonatal Brain Hypoxia-Ischemia

Shao

Wang²,

Guan³

et al. 2017

Dev Neurosci

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“…In this review, we have focused on exocytic and endocytic machinery and the role the Golgi and ER play in controlling plasma membrane expansion and receptor dynamics. Recent studies have identified possible molecules and mechanisms which may bridge these cell biological functions including TRIM9, PACSINS, JIP1, and cytoskeletal motor proteins which transport vesicles (Deng et al, 2014; Kessels, 2004; Winkle et al, 2014; Zhang et al, 2004; Zhu et al, 2007). This is an area awaiting further investigation.…”

Section: Discussionmentioning

confidence: 99%

Membrane Trafficking in Neuronal Development: Ins and Outs of Neural Connectivity

Winkle

Gupton

2016

International Review of Cell and Molecular Biology

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During development, neurons progress through rapid yet stereotypical shape changes to achieve proper neuronal connectivity. This morphological progression requires carefully orchestrated plasma membrane expansion, insertion of membrane components including receptors for extracellular cues into the plasma membrane and removal and trafficking of membrane materials and proteins to specific locations. This review outlines the cellular machinery of membrane trafficking that play an integral role in neuronal cell shape change and function from initial neurite formation to pathway navigation and synaptogenesis.

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“…Recently, we have shown that Rab10 is associated with PPVs [24], Myosin V controls biogenesis of postGolgi Rab10 carriers [27], and JIP1 mediates anterograde transport of Rab10-PPVs during axon development [56]. How do Rab10 vesicles dock and fuse with the membrane at the destination sites?…”

Section: Discussionmentioning

confidence: 99%

MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development

Deng

Liu

et al. 2014

Cell Res

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Axon development requires membrane addition from the intracellular supply, which has been shown to be mediated by Rab10-positive plasmalemmal precursor vesicles (PPVs). However, the molecular mechanisms underlying the membrane trafficking processes of PPVs remain unclear. Here, we show that myristoylated alanine-rich C-kinase substrate (MARCKS) mediates membrane targeting of Rab10-positive PPVs, and this regulation is critical for axon development. We found that the GTP-locked active form of Rab10 binds to membrane-associated MARCKS, whose affinity depends on the phosphorylation status of the MARCKS effector domain. Either genetic silencing of MARCKS or disruption of its interaction with Rab10 inhibited axon growth of cortical neurons, impaired docking and fusion of Rab10 vesicles with the plasma membrane, and consequently caused a loss of membrane insertion of axonal receptors responsive to extracellular axon growth factors. Thus, this study has identified a novel function of MARCKS in mediating membrane targeting of PPVs during axon development.

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JIP1 Mediates Anterograde Transport of Rab10 Cargos during Neuronal Polarization

Cited by 50 publications

References 59 publications

Proteomic Analysis of Mouse Cortex Postsynaptic Density following Neonatal Brain Hypoxia-Ischemia

Proteomic Analysis of Mouse Cortex Postsynaptic Density following Neonatal Brain Hypoxia-Ischemia

Membrane Trafficking in Neuronal Development: Ins and Outs of Neural Connectivity

MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development

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