Jian-Pi-Yi-Shen Formula Regulates Inflammatory Cytokines Production in 5/6 Nephrectomized Rats via Suppression of NF-κB Activation

Abstract

Jian-Pi-Yi-Shen formula (JPYSF) is a Chinese herbal decoction used for treating chronic kidney disease (CKD) for over 20 years with good efficiency. However, the mechanism lacks solid evidence. In the present study, we tested the hypothesis that JPYSF may retard CKD progression via inhibition of inflammation in 5/6 nephrectomy (5/6 Nx) rat model. The 5/6 Nx rats were randomly divided into 2 groups: 5/6 Nx group and JPYSF group. Sham-operated rats served as control. JPYSF (2.06 g/kg/d) were administrated by gavage to 5/6 Nx rats daily for 6 weeks. Results showed that JPYSF treatment significantly improved kidney function and pathological injury in 5/6 Nx rats. Multiplex analysis of cytokines revealed that JPYSF reduced proinflammatory cytokines and increased anti-inflammatory cytokine production. Furthermore, JPYSF inhibited the activation of nuclear factor-kappa B (NF-κB) signaling pathway. In conclusion, our data demonstrated that JPYSF remarkably retards development and progression of CKD in a 5/6 Nx rat model, which may be associated with inhibition of inflammation via NF-κB signaling pathway.

Jian-Pi-Yi-Shen Decoction Relieves Renal Anemia in 5/6 Nephrectomized Rats: Production of Erythropoietin via Hypoxia Inducible Factor Signaling

“…These effects could be involved with the production of EPO via HIF signaling pathway. In line with these findings, our previous studies revealed that JPYS remarkably retards development and progression of CKD in animal model and induced expression of EPO in cultured kidney cells [9, 10, 12]. The results of this study demonstrated that the serum EPO levels were decreased in CKD rats.…”

Jian-Pi-Yi-Shen Regulates EPO and Iron Recycling Protein Expressions in Anemic Rats with Chronic Kidney Disease: Accumulation of Hypoxia Inducible Factor-2α via ERK Signaling

“…Besides, the overexpression of serum hepcidin reverted to its original level by JPYS treatment, ensuring the systemic and intracellular iron homeostasis. In line with this notion, previous studies showed that JPYS could suppress proinflammatory cytokines production in 5/6 nephrectomized rats [ 36 ]. The hepcidin expression in the liver can be induced by the upregulation of cytokines, most notably IL-6 and LPS [ 27 ], whereas the increased systemic hepcidin level can act on macrophages, preventing the release of iron recovered from senescent red blood cells into plasma [ 37 ].…”

Metabolomics Analysis Reveals the Protection Mechanism of Huangqi–Danshen Decoction on Adenine-Induced Chronic Kidney Disease in Rats

“…Sections were stained with periodic acid–Schiff (PAS) and Masson’s trichrome stains. The degree of tubular atrophy in PAS staining was determined based on a scale of 0–3 points: 0, normal tubules; 1, rare single atrophic tubule; 2, several clusters of atrophic tubules; and 3, massive atrophy (Lu et al, 2018). Interstitial fibrosis area in Masson staining was measured using Image J software (NIH, Bethesda, MD, USA).…”