Jerusalem artichoke invertases—Immunocharacterization of a soluble form and its putative precursor

Goupil, Pascale; Croisille, Y; Croisille, F.; Ledoigt, Gérard

doi:10.1016/0168-9452(88)90054-4

Cited by 12 publications

(2 citation statements)

References 19 publications

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“…The 62-kD estimated size is well within the predicted range for this protein, based on deduced amino acid sequence (72 kD deduced from the cDNA and 65 kD deduced from a putative cleavage site at the N terminus, predicted by the SignalP V1.1 program) and other soluble type invertases. Examples of SDS-PAGE estimates of other purified soluble invertases include Lilium longiflorum flower buds with isoforms at 78, 54, 52, and 24 kD (Ranwala et al, 1998), carrot dry seeds and seedlings with a 68-kD polypeptide and proteolytic fragments at 43 and 25 kD (Unger et al, 1992), elongating stem tissue from barley at 60 kD (Karuppiah et al, 1989), Jerusalem artichoke shoot extracts at 58 kD (Goupil et al, 1988), and wheat coleoptiles at 53 kD (Krishnan et al, 1985). Somewhat smaller vacuolar forms of invertase have also been reported, including a 35-kD polypeptide from potato tuber (Isla et al, 1998) and a 42-kD protein from sweet pepper (Michaud et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

A Re-Evaluation of the Relative Roles of Two Invertases, INCW2 and IVR1, in Developing Maize Kernels and Other Tissues

Carlson

Chourey

1999

Plant Physiology

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We have examined the relative abundance and distribution of the transcripts and protein products of a cell wall gene (Incw2) and a soluble invertase gene (Ivr1) to better understand their relative roles during maize (Zea mays L.) kernel development. In developing kernels the steady-state levels of Incw2 transcript increased dramatically from 0 to 12 d after pollination, while Ivr1 transcript, in contrast to a previous report, was undetectable. Consistent with the RNA expression data, the IVR1 protein could not be detected in kernel extracts using antisera raised to a synthetic peptide. Fractionation of the soluble form of invertase from developing kernels by isoelectric focusing and protein blots suggested that the enzyme activity was due to contamination of the cell wall invertase protein.A similar observation was made in a maize cell suspension culture in which Ivr1 RNA, but not IVR1 protein, was significantly modulated by sugars in the medium. Protein-blot analyses of the soluble enzyme activity suggested that changes in the enzyme activity are attributable to a cell wall invertase protein in the soluble fraction. Based on the collective evidence, we propose that the cell wall, but not the soluble invertase, is critical to heterotrophic sinks such as cell suspension cultures and developing kernels.

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Section: Discussionmentioning

confidence: 99%

A Re-Evaluation of the Relative Roles of Two Invertases, INCW2 and IVR1, in Developing Maize Kernels and Other Tissues

Carlson

Chourey

1999

Plant Physiology

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“…Recently, a number of higher plant cell wall invertases (EC 3.2.1.26) have been purified and characterized (3,5,6,9,13,15,17,(22)(23)(24)37). The enzyme, which has an acidic pH optimum and is positively charged (pI 9-10) at the pH of the cell wall, hydrolyzes sucrose in the apoplast in rapidly growing tissues and has been proposed to be involved in establishing metabolic sinks (25,33, and literature cited therein).…”

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confidence: 99%

Cell Wall Invertase in Tobacco Crown Gall Cells

Weil¹,

Rausch²

1990

Plant Physiol.

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The cell wall invertase from an Agrobacterium tumefacienstransformed Nkotiana tabacum cell line (SR1-C58) was purified. The heterogeneously glycosylated enzyme has the following properties: M. 63,000, pH optimum at 4.7, Km ase 0.6 millimolar (at pH 4.7), pi 9.5. Enzyme activity is inhibited by micromolar concentrations of HgCI2 but is insensitive to H202, N-ethylmaleimide and dithiothreitol. Upon transfer of transformed cells from the stationary phase to fresh medium, a cycloheximide-and tunicamycin-sensitive de novo formation of cell wall invertase is demonstrated in the absence or presence of sucrose. While in an auxin mutant (lacking gene 1; SR1-3845) 1 micromolar 1-naphthaleneacetic acid led to a further increased activity, the wild-type transformed cell line (SRI-C58) responded with a decreased activity compared to the control. An analysis of cell wall invertase in and around tumors initiated with Agrob.cteium tumefaciens (strain C58) on Nkotlana tabacum stem and Kalancho6 daigremontlana leaves revealed gradients of activity. The results indicate that the auxin-stimulated cell wall invertase is essential for the establishment of the tumor sink.about the factors involved in gene regulation. In view of the established effects of auxin on expression of several cell wall proteins (32), we started from the hypothesis that synthesis and/or secretion of cell wall invertase may be regulated by this hormone. Earlier reports suggested that an exogenous application of auxin and gibberellic acid stimulates invertase activity, but the specificity of the effect for the cell wall form was not demonstrated (25). As a model system, we used

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Beta-fructofuranosidase

Schomburg

Salzmann

1991

Enzyme Handbook 4

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Jerusalem artichoke invertases—Immunocharacterization of a soluble form and its putative precursor

Cited by 12 publications

References 19 publications

A Re-Evaluation of the Relative Roles of Two Invertases, INCW2 and IVR1, in Developing Maize Kernels and Other Tissues

A Re-Evaluation of the Relative Roles of Two Invertases, INCW2 and IVR1, in Developing Maize Kernels and Other Tissues

Cell Wall Invertase in Tobacco Crown Gall Cells

Beta-fructofuranosidase

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