Jeotgalicoccus marinus sp. nov., a marine bacterium isolated from a sea urchin

Abstract

A novel non-sporulating, non-motile, catalase-and oxidase-positive, facultatively anaerobic, moderately halophilic, Gram-positive coccus, designated JSM 076033 T , was isolated from a sea urchin (Hemicentrotus pulcherrimus) collected from the South China Sea. Strain JSM 076033 T was able to grow in the presence of 0.5-25.0 % (w/v) total salts and at pH 6.0-10.0 and 10-45 6C; optimum growth was observed with 5.0-10.0 % (w/v) total salts and at pH 7.0-8.0 and 25-30 6C. The major amino acid constituents of the cell wall were glycine, lysine and alanine. The major cellular fatty acids were anteiso-C 15 : 0 , iso-C 15 : 0 and anteiso-C 17 : 0 . The respiratory quinones were MK-7 (60.7 %) and MK-6 (39.3 %) and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid. The DNA G+C content was 40.3 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 076033 T should be assigned to the genus Jeotgalicoccus.The sequence similarities between the novel isolate and the type strains of recognized Jeotgalicoccus species were in the range 95. et al., 2003). During an investigation of the diversity of the microbial population of invertebrates inhabiting the South China Sea, we isolated a novel moderately halophilic bacterium, designated JSM 076033 T , from a sea urchin (Hemicentrotus pulcherrimus) collected from Leizhou Bay, China. On the basis of the results of a polyphasic taxonomic analysis, this strain represents a novel species of the genus Jeotgalicoccus.For strain isolation, serial dilutions (1 : 10) of homogenates of the sea urchin were plated on Difco marine agar 2216 (MA; pH 7.5), supplemented with 0-20 % (w/v) NaCl, at 28 u C for 7-28 days. A creamy-white-pigmented colony, designated strain JSM 076033 T , was picked from a plate of MA supplemented with 10 % (w/v) NaCl. It was maintained both on slants of MA supplemented with 5 % (w/v) NaCl (referred to as MA5) at 4 u C and in Difco marineThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 076033 T is EU583727.A supplementary table showing the fatty acid compositions of strain JSM 076033 T and members of the genus Jeotgalicoccus is available with the online version of this paper.

Terminal Olefin (1-Alkene) Biosynthesis by a Novel P450 Fatty Acid Decarboxylase from Jeotgalicoccus Species

“…The sequence was classified using the Ribosomal Database Project website (http://rdp.cme.msu .edu/) with a 100% confidence threshold as Jeotgalicoccus spp. It showed 99% sequence identity to the 16S rRNA genes from Jeotgalicoccus marinus JSM 076033 (12) and Jeotgalicoccus sp. strain NY-2 (26).…”

Jeotgalicoccus coquinae sp. nov. and Jeotgalicoccus aerolatus sp. nov., isolated from poultry houses

“…Later, Hoyles et al (2004) isolated a third species, Jeotgalicoccus pinnipedialis, from a southern elephant seal. Jeotgalicoccus marinus was described by Chen et al (2009) and was isolated from a sea urchin, and Guo et al (2010) isolated Jeotgalicoccus huakuii from a seaside soil in China.…”

Jeotgalicoccus halophilus sp. nov., isolated from salt lakes

“…A third species, Jeotgalicoccus pinnipedialis, from the oral cavity of a southern elephant seal, was subsequently described by Hoyles et al (2004). To date, two more species have been described within this genus: Jeotgalicoccus marinus (Chen et al, 2009), from a sea urchin collected from the South China Sea, and Jeotgalicoccus huakuii (Guo et al, 2010), from a seaside soil sample in Shandong province of China. The main characteristics of this genus are Gram-positive, non-motile, non-endospore-forming, catalase-and oxidase-positive cocci.…”