Jellyfish collagen and alginate: Combined marine materials for superior chondrogenesis of hMSC

Pustlauk, Wera; Paul, Birgit; Gelinsky, Michael; Bernhardt, Anne

doi:10.1016/j.msec.2016.03.081

Cited by 59 publications

(34 citation statements)

References 67 publications

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“…The capacity of the collagen to promote cell migration in wounded tissue suggests its possible use in synthetic matrix production for cartilage tissue engineering, mainly fibrous, hydrogel or hybrid materials [125,126]. Several efforts have been focused on collagen-based material colonization with cells of connective tissue such as fibroblasts or endothelial cells and other growth supports, in an attempt to produce a similar biocompatibility and immune response with collagen-based material commercially available.…”

Section: Jellyfishmentioning

confidence: 99%

Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications

et al. 2020

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Due to its unique properties, collagen is used in the growing fields of pharmaceutical and biomedical devices, as well as in the fields of nutraceuticals, cosmeceuticals, food and beverages. Collagen also represents a valid resource for bioplastics and biomaterials, to be used in the emerging health sectors. Recently, marine organisms have been considered as promising sources of collagen, because they do not harbor transmissible disease. In particular, fish biomass as well as by-catch organisms, such as undersized fish, jellyfish, sharks, starfish, and sponges, possess a very high collagen content. The use of discarded and underused biomass could contribute to the development of a sustainable process for collagen extraction, with a significantly reduced environmental impact. This addresses the European zero-waste strategy, which supports all three generally accepted goals of sustainability: sustainable economic well-being, environmental protection, and social well-being. A zero-waste strategy would use far fewer new raw materials and send no waste materials to landfills. In this review, we present an overview of the studies carried out on collagen obtained from by-catch organisms and fish wastes. Additionally, we discuss novel technologies based on thermoplastic processes that could be applied, likewise, as marine collagen treatment.2 of 23 protective colloid functions and film-forming capacity. Also, collagen is a good surface-active agent, with its ability to penetrate lipid-free interfaces [2].Collagen can be utilized in a variety of applications because of its biocompatibility and excellent degradability [3,4]. Furthermore, it is known that collagen is a molecule with weak immunogenicity, which decreases the possibilities of rejection when it is ingested or injected into a different body. Although this molecule has already low antigenicity, this property can be enhanced by modifying it to suppress any immune response [5,6]. Additionally, collagen peptides and gelatin (denatured collagen) have been widely utilized in different fields such as food, medicine, cosmetics, leather and film industries, diagnostic imaging, and therapeutic delivery [7].For many years, most available collagen was extracted from discards from the bovine and porcine processing industries, but during the last few decades the use of collagen from these sources has been limited. The use of porcine and bovine-derived products is occasionally prevented by dietary regimes, due to specific needs or personal choices. It is forbidden by religious constraints, to Muslims, Hindus and Jews who make up 38.4% of the global population [8]. Moreover, the use of bovine-derived products became a concern for a wider section of the population during the bovine spongiform encephalopathy (BSE), transmissible spongiform encephalopathy (TSE) and foot-and-mouth disease (FMD) crises that occurred over the last few decades in all areas of the world, mostly in the United Kingdom and Asia. Bovine-derived products might be a vehicle of transmission of these diseases ...

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Section: Jellyfishmentioning

confidence: 99%

Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications

et al. 2020

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“…This is why Pustlauk et al [28] investigated their potential for joint cartilage repair. They studied the expression of the COL 2 gene and found that its expression was comparable in all scaffold types examined.…”

Section: Collagen Electrospinningmentioning

confidence: 99%

Gelatin and Collagen Nanofiber Scaffolds for Tissue Engineering

Monroy¹,

Bravo²,

Mercado³

et al. 2018

Tissue Regeneration

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One of the main complications that can present a person with second and third degree burns is the possibility of being infected by opportunistic bacteria or viruses that are present in the environment. Nowadays, the majority of the burn injuries are treated with conventional gauze, which involves a high probability of infection and pain for the patient being treated with this method. In order to obtain low-cost scaffolds, natural and abundant polymers were used such as gelatin (GEL) and collagen (COL). The GEL functions as a base scaffold, stable and flexible, and also biocompatible because it is a byproduct of the partial hydrolysis of COL, which is an indispensable component for the stability of the cell membrane and it is present in great extent in the human epithelium.

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“…At day 21, however, a much more distinct, pro-chondrogenic effect is demonstrated in the PEU scaffolds, with lower type I collagen expression and higher expression of aggrecan and type II collagen. Combined, these data suggest a trend in gene expression that potentially demonstrates PEU better supports a more long-term chondrogenic phenotype in TC28a2 human chondrocytes than PCL [ 53 , 54 ]. This is likely due to chemical and mechanical differences between the two materials.…”

Section: Resultsmentioning

confidence: 99%

A Preliminary Evaluation of the Pro-Chondrogenic Potential of 3D-Bioprinted Poly(ester Urea) Scaffolds

et al. 2020

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Degeneration of articular cartilage (AC) is a common healthcare issue that can result in significantly impaired function and mobility for affected patients. The avascular nature of the tissue strongly burdens its regenerative capacity contributing to the development of more serious conditions such as osteoarthritis. Recent advances in bioprinting have prompted the development of alternative tissue engineering therapies for the generation of AC. Particular interest has been dedicated to scaffold-based strategies where 3D substrates are used to guide cellular function and tissue ingrowth. Despite its extensive use in bioprinting, the application of polycaprolactone (PCL) in AC is, however, restricted by properties that inhibit pro-chondrogenic cell phenotypes. This study proposes the use of a new bioprintable poly(ester urea) (PEU) material as an alternative to PCL for the generation of an in vitro model of early chondrogenesis. The polymer was successfully printed into 3D constructs displaying adequate substrate stiffness and increased hydrophilicity compared to PCL. Human chondrocytes cultured on the scaffolds exhibited higher cell viability and improved chondrogenic phenotype with upregulation of genes associated with type II collagen and aggrecan synthesis. Bioprinted PEU scaffolds could, therefore, provide a potential platform for the fabrication of bespoke, pro-chondrogenic tissue engineering constructs.

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Jellyfish collagen and alginate: Combined marine materials for superior chondrogenesis of hMSC

Cited by 59 publications

References 67 publications

Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications

Marine Collagen from Alternative and Sustainable Sources: Extraction, Processing and Applications

Gelatin and Collagen Nanofiber Scaffolds for Tissue Engineering

A Preliminary Evaluation of the Pro-Chondrogenic Potential of 3D-Bioprinted Poly(ester Urea) Scaffolds

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