Jellyfish Bioactive Compounds: Methods for Wet-Lab Work

Frazão, Bárbara; Antunes, Agostinho

doi:10.3390/md14040075

Cited by 21 publications

(18 citation statements)

References 111 publications

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“…Moreover, the sulfur content of PULV (9.67%) was similar to purified fucoidan (9.83%) obtained from Fucus vesiculosus. [25] The carbon content of PGCL (29.49%) is significantly higher compared to purified fucoidan (26.12%) of F. vesiculosus. [25] Functional group analysis…”

Section: Carbohydrate Content and Elemental Compositionmentioning

confidence: 90%

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Chemical characterization of sulfated polysaccharides from Gracilaria gracilis and Ulva lactuca and their radical scavenging, metal chelating, and cholinesterase inhibitory activities

Olasehinde

Mabinya

Olaniran

et al. 2019

International Journal of Food Properties

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This study was designed to evaluate the antioxidant and cholinesterase inhibitory activities of sulfated polysaccharides from Gracilaria gracilis (PGCL) and Ulva lactuca (PULV). PGCL and PULV were extracted and characterized with Scanning electron microscope-energy dispersive x-ray (SEM-EDX), Fourier transform infrared spectroscopy (FTIR) and Gas chromatography-mass spectroscopy (GC-MS). The radical (2,2-azinobis-3-ethylbenzothiazoline-6-sulfonate [ABTS] 2,2-diphenyl-1-picrylhydrazyl [DPPH], and hydroxyl [OH]) scavenging and metal chelating activities as well as their inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities were also determined. SEM-EDX analysis revealed the presence of elements such as carbon, magnesium, calcium, phosphorus, and sulfur. The FTIR analysis also confirmed the presence of sulfate group in the polysaccharide samples. Some sugars such as glucose, galactose, arabinose, xylose, rhamnose, and allose were also identified using GC-MS. PGCL and PULV scavenged ABTS, DPPH and OH radicals in a dose-dependent manner. Moreover, PULV exhibited higher scavenging activity compared to PGCL. PGCL and PULV also reduced the activity of AChE (IC 50 = 132.73 and 106.93 µg/mL) and BChE (IC 50 = 124.93 and 93.45 µg/mL) respectively. PGCL and PULV exhibited antioxidant activity and possess cholinesterase inhibitory activity, hence these polysaccharides could be explored as ingredients for the development of functional foods with neuroprotective potentials.

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Section: Carbohydrate Content and Elemental Compositionmentioning

confidence: 90%

“…[25] The carbon content of PGCL (29.49%) is significantly higher compared to purified fucoidan (26.12%) of F. vesiculosus. [25] Functional group analysis…”

Section: Carbohydrate Content and Elemental Compositionmentioning

confidence: 90%

Chemical characterization of sulfated polysaccharides from Gracilaria gracilis and Ulva lactuca and their radical scavenging, metal chelating, and cholinesterase inhibitory activities

Olasehinde

Mabinya

Olaniran

et al. 2019

International Journal of Food Properties

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“…The details of blast search and protein identification by MALDI-TOF/TOF mass spectrometry of the protein identified from the 2DE is shown in Table 1 . It is noteworthy, that some of the proteins identified have been previously reported in other cnidarians [ 46 , 47 , 48 ], but constitute the first report in B. verrucosa .…”

Section: Resultsmentioning

confidence: 86%

Proteomic Analyses of the Unexplored Sea Anemone Bunodactis verrucosa

et al. 2018

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Cnidarian toxic products, particularly peptide toxins, constitute a promising target for biomedicine research. Indeed, cnidarians are considered as the largest phylum of generally toxic animals. However, research on peptides and toxins of sea anemones is still limited. Moreover, most of the toxins from sea anemones have been discovered by classical purification approaches. Recently, high-throughput methodologies have been used for this purpose but in other Phyla. Hence, the present work was focused on the proteomic analyses of whole-body extract from the unexplored sea anemone Bunodactis verrucosa. The proteomic analyses applied were based on two methods: two-dimensional gel electrophoresis combined with MALDI-TOF/TOF and shotgun proteomic approach. In total, 413 proteins were identified, but only eight proteins were identified from gel-based analyses. Such proteins are mainly involved in basal metabolism and biosynthesis of antibiotics as the most relevant pathways. In addition, some putative toxins including metalloproteinases and neurotoxins were also identified. These findings reinforce the significance of the production of antimicrobial compounds and toxins by sea anemones, which play a significant role in defense and feeding. In general, the present study provides the first proteome map of the sea anemone B. verrucosa stablishing a reference for future studies in the discovery of new compounds.

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“…[40][41][42] Moreover, inhibition of PI3K/Akt signaling pathway has been used as a therapeutic strategy to treat gastric cancer patients. 43 Evidences of ROS1 targeted PI3K/Akt signaling pathway existed in some aspects. ROS1 was reported to be involved in BMSC fate switching between osteogenesis and adipogenesis, mediated via PI3K/AKT/mTORC1 signaling.…”

Section: Discussionmentioning

confidence: 99%

<p>Knockdown of ROS proto-oncogene 1 inhibits migration and invasion in gastric cancer cells by targeting the PI3K/Akt signaling pathway</p>

Jian¹,

Sun³

et al. 2019

OTT

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Objectives: Gastric cancer ranks the fourth most common cancer and the third leading cause of cancer mortality in the world. ROS proto-oncogene 1 (ROS1) is an oncogene and ROS1 rearrangement has been reported in many cancers. Our study aimed to investigate the potential function and the precise mechanisms of ROS1 in gastric cancer. Methods: In our study, the analysis of ROS1 expression and clinical pathologic factors of gastric cancer in gastric cancer using TCGA database demonstrated that ROS1 expression was elevated in gastric cancer and related to T, N, M and TNM staging. High expression of ROS1 predicted poor survival in patients with gastric cancer. Then, we measured ROS1 expression in four human gastric cancer cell lines and knocked down ROS1 expression in BGC-823 and SGC-7901 cells by specific shRNA transfection via Lipofectamine 2000. The effect of ROS1 knockdown on cell proliferation, cell cycle distribution, cell apoptosis and metastasis in vitro was evaluated by MTT, colony formation, flow cytometric analysis, wound healing and Transwell invasion assays. The levels of apoptosis-related proteins, EMT markers and the PI3K/Akt signaling pathway members were measured by Western blotting. Results: We demonstrated that shROS1 transfection markedly downregulated ROS1 expression in BGC-823 and SGC-7901 cells. Knockdown of ROS1 inhibited cell survival, clonogenic growth, migration, invasion and epithelial-mesenchymal transition (EMT), as well as induced cell cycle arrest and apoptosis in gastric cancer cells. Furthermore, ROS1 knockdown inhibited the phosphorylation of PI3K and Akt. Conclusion: Collectively, our data suggest that ROS1 may serve as a promising therapeutic target in gastric cancer treatment.

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Jellyfish Bioactive Compounds: Methods for Wet-Lab Work

Cited by 21 publications

References 111 publications

Chemical characterization of sulfated polysaccharides from Gracilaria gracilis and Ulva lactuca and their radical scavenging, metal chelating, and cholinesterase inhibitory activities

Chemical characterization of sulfated polysaccharides from Gracilaria gracilis and Ulva lactuca and their radical scavenging, metal chelating, and cholinesterase inhibitory activities

Proteomic Analyses of the Unexplored Sea Anemone Bunodactis verrucosa

<p>Knockdown of ROS proto-oncogene 1 inhibits migration and invasion in gastric cancer cells by targeting the PI3K/Akt signaling pathway</p>

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