Jelly Layer Formation in Penaeoidean Shrimp Eggs

Clark, Wallis H.; Yudin, Ashley I.; Lynn, John W.; Griffin, Frederick J.; Pillai, Muralidharan C.

doi:10.2307/1541831

Cited by 54 publications

(30 citation statements)

References 13 publications

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“…Gamete mixing is crucial for two reasons; first, since sperm are nonmotile, their contact with ova needs to be facilitated by the female (27). Second, since sea water Mg*+-initiated oocyte activation (32, 39) results in jelly precursor release and VE dissipation (15), any sperm that do not come into contact with ova minutes after spawning are unable to undergo primary binding. VE ligands involved in sperm binding therefore are important not only in rapidly "capturing" these nonmotile sperm but are also important for sperm orientation.…”

Section: Discussionmentioning

confidence: 99%

Two Extracellular Matrices From Oocytes of the Marine Shrimp Sicyonia ingentis that Independently Mediate Only Primary or Secondary Sperm Binding

Wikramanayake

Clark

1994

Dev Growth Differ

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During spawning, female Sicyonia ingentis simultaneously release ova and stored nonmotile sperm and mix them externally to initiate gamete interaction. Sperm bind to a thin vitelline envelope (VE) via their anterior appendage and within seconds are induced to undergo acrosomal exocytosis. The sperm penetrate the VE and become secondarily bound to the surface coat (SC), a glycocalyx on the oocyte surface. In this study, both extracellular matrices were isolated from S. ingentis oocytes. Isolated VEs mediated only primary sperm binding (i.e., before the acrosome reaction), while the isolated SCs mediated only secondary sperm binding (i.e., after acrosomal exocytosis). Isolated S. ingentis VEs were used to characterize primary sperm binding activity. The two extracellular matrices differ morphologically and possess different polypeptide profiles. Soluble fractions of isolated VEs inhibited primary sperm binding in a concentration dependent manner, and immunolocalization of VE components demonstrated highly localized VE binding sites at the tip of the sperm anterior appendage by which sperm bind eggs. Extensive Pronase digestion of VE components did not affect sperm binding activity of solubilized VE components, while complete deglycosylation with trifluoromethanesulfonic acid destroyed sperm binding activity. However, neither alkaline treatment nor enzyme digestion using glycosidases specific for asparagine and serine/ threonine linked oligosaccharides affected sperm binding activity.

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Section: Discussionmentioning

confidence: 99%

Two Extracellular Matrices From Oocytes of the Marine Shrimp Sicyonia ingentis that Independently Mediate Only Primary or Secondary Sperm Binding

Wikramanayake

Clark

1994

Dev Growth Differ

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“…In penaeid shrimps, ovarian development is characterized by the formation of cortical rods in the oocytes after the completion of yolk accumulation (Clark et al 1990). As in Macrobrachium prawns and shrimp, oocyte development is classified into the previtellogenic, endogenous vitellogenic, and exogenous vitellogenic stages (Fig.…”

Section: -3 Modes Of Ovarian Maturationmentioning

confidence: 99%

“…In penaeid shrimps, after the completion of yolk accumulation, cortical rods are formed radially around the periphery of the oocyte plasma membrane (Clark et al 1990;Wallis et al 1990). Two major proteins comprising the cortical rods have been purified and characterized: shrimp ovarian peritrophin (SOP, 29-35 and 33-36 kDa) in Penaeus semisulcatus (Khayat et al 2001) or cortical rod protein (CRP, 28.6 and 30.5 kDa) in Mar.…”

Section: -3b Identification Of Cortical Rod Proteins In Penaeid Spementioning

confidence: 99%

“…Taken together with the fact that SOP, CRP, and MjTSP genes are transcribed and translated at different oocyte stages, these results indicate that expression of these genes is controlled at the translational level by CHH-family peptides. The content of the cortical rods is released around eggs and forms into a jelly layer, which envelopes the eggs on contact with sea water at spawning (Clark et al 1990). The jelly layer starts dissipating around 45 minutes after spawning, and the hatching envelope forms on the egg surface.…”

Section: -3b Identification Of Cortical Rod Proteins In Penaeid Spementioning

confidence: 99%

“…The jelly layer starts dissipating around 45 minutes after spawning, and the hatching envelope forms on the egg surface. The jelly layer is considered to function as a barrier against polyspermy and as an external protective coat from the time of spawning to the formation of the hatching envelope (Clark et al 1990). Recently, a CRP homologue (mrCRP, 30 kDa) was found in the ovaries of Mac.…”

Section: -3b Identification Of Cortical Rod Proteins In Penaeid Spementioning

confidence: 99%

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Reproductive Mechanisms in Crustacea Focusing on Selected Prawn Species: Vitellogenin Structure, Processing and Synthetic Control

Wilder¹,

Okumura²,

Tsutsui³

2010

Aqua-BioSci. Monogr. (ABSM)

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Shrimp culture is a significant world-wide industry, with current production levels reaching over 3 million tons per year. The expansion of the industry has given rise to the problems of environmental deterioration due to intensive-scale culture, and the outbreak of disease. While many of these issues are now being sufficiently addressed, the establishment of sustainable seed production technology is an area that should be given continued attention. In this regard, it remains difficult to control reproduction under hatchery conditions for a large number of commercially-important species. At present, an understanding of reproductive mechanisms in Crustacea is not complete, although in recent years, a great deal of knowledge has accumulated on vitellogenin structure, processing, and synthetic site in a number of economically-important species. This monograph will cover the current status of research on vitellogenin in decapod crustaceans, especially prawns and shrimp, and discuss mechanisms of vitellogenin synthetic control, both demonstrated and postulated. The monograph will also present current knowledge of crustacean vitellogenin receptors, and cover related facets of reproductive development, such as mechansisms of cortical rod formation and the utilization of vitellin during embryogenesis. Finally, future directions for this research and potential applications to aquaculture will be discussed.

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Egg coats of the rock shrimp Rhynchocinetes typus: ultrastructural characterization and their function during fertilization

et al. 2002

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The aim of the present work was to characterize structurally and ultrastructurally the egg coats of the rock shrimp, Rhynchocinetes typus, and to describe their functional roles during fertilization. Oocytes fixed directly from the ovary, have a total diameter of 549 microm and are covered by a 10- microm-thick transparent envelope. Electron microscope sections (dehydrated) of the egg envelope revealed an electron-dense external coat of 0.4 microm covered by filamentous processes, and a granular inner coat of 4- microm thickness. Oocytes placed for 5 min in seawater had a significantly larger diameter (573 microm), because of the increase in the thickness of the egg coats (32 microm) and the formation of a 16- microm perivitelline space. The diameter of the egg proper was reduced by the same extent as the size of the perivitelline space. All these changes were associated to the loss of the egg fertilizability. SDS-PAGE of isolated and solubilized egg coats with 20% beta-mercaptoethanol or 25 mM dithiothreitol (DTT) showed bands between 58 and 105 kDa and between 44 and 103 kDa, respectively. During normal fertilization, the sperm undergoes a drastic change in shape after first contact with the egg. We observed a similar change when solubilized egg coats were placed with vas deferens sperms. When the solubilized egg coat proteins were ultrafiltrated with a membrane of 10,000 MWCO (pore size) and then assayed for their effect on fertilization, an inhibitory effect of 30%, 41%, and 59% was found when oocytes were incubated with spermatozoa pre-treated with 30, 60, and 120 microg/ml of proteins solubilized with beta-mercaptoethanol. A similar inhibitory effect was found when egg coat proteins solubilized with 25 mM DTT were used. Our results suggest that, in the shrimp, the egg coats play an active role during the morphological changes of the sperm during their passage through them.

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Jelly Layer Formation in Penaeoidean Shrimp Eggs

Cited by 54 publications

References 13 publications

Two Extracellular Matrices From Oocytes of the Marine Shrimp Sicyonia ingentis that Independently Mediate Only Primary or Secondary Sperm Binding

Two Extracellular Matrices From Oocytes of the Marine Shrimp Sicyonia ingentis that Independently Mediate Only Primary or Secondary Sperm Binding

Reproductive Mechanisms in Crustacea Focusing on Selected Prawn Species: Vitellogenin Structure, Processing and Synthetic Control

Egg coats of the rock shrimp Rhynchocinetes typus: ultrastructural characterization and their function during fertilization

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