Jejunal Bacterial Flora in Chronic Small Bowel Disease

Prizont, Roberto; Hersh, Theodore; Floch, Martin H.

doi:10.1093/ajcn/23.12.1602

Cited by 36 publications

(18 citation statements)

References 12 publications

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“…This operation increased the stability of the enzyme and allowed determinations to be made with short assay times (<30 min). Furthermore, because the growth of organisms did not exceed 106-107/ml in culture, a 10-fold increase in activity would be consistent with protease that might be secreted from 109-1010 organisms found in the blind loop syndrome (7,(31)(32)(33)(34). Proteolysis in the concentrated form did not increase after removal by dialysis of the concentrated salt resulting from lyophilization.…”

Section: Resultssupporting

confidence: 56%

“…Variations in the levels of brush border hydrolases in rats-e.g., sucrase, maltase, lactase, and alkaline phosphatase-have been evaluated most extensively (1,2,4,5). Similar changes have been demonstrated in human bacterial overgrowth syndromes (6,7). Several hypotheses explain the mechanism of the bowel mucosal damage, such as direct damage by bacteria (1)(2)(3)(4) or their secreted products, or changes in the luminal environment-e.g., effects of secondary bile acids (deoxycholic) with inhibition or solubilization of surface enzymes (8)(9)(10).…”

Section: Introductionmentioning

confidence: 94%

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Effect of Secreted Bacteroides Proteases on Human Intestinal Brush Border Hydrolases

Riepe¹,

Goldstein²,

Alpers³

1980

J. Clin. Invest.

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A B S T R A C T Selected bacteroides species secreted various amounts of protease and glycosidase into their growth medium. Bacteroides vulgatus, distasonis, and ovatus secreted the most (31-60% of total). The secreted protease was similar in action to the protease within the organism, in that it had a broad pH optimum of 6-9, a Km app. for casein of 0.1 ,uM, and was inhibited by benzamidine, phenylmethylsulfonyl fluoride, diisopropylfluorophosphate (DIFP), and by an elastase inhibitor, Ac(Ala)3AlaCH2Cl.Exposure of human brush border preparations to the secreted protease reduced maltase and sucrase activities; the reduction could be prevented by DIFP. In contrast, brush border alkaline phosphatase activity either did not change or increased after exposure to bacterial secretions. >90% inhibition of secreted glycosidase using EDTA and p-chloromercuribenzoic acid did not prevent the reduction of brush border maltase and sucrase activity, suggesting that glucosidases were not likely to be involved in the destruction of brush border enzymes. Moreover, the bacterial proteases caused only a small net release of active maltase or sucrase from the brush border. Most of the loss of activity was due to destruction of the enzyme. Proximal bowel fluid of three patients with overgrowth contained DIFP-inhibitable protease that destroyed sucrase in isolated brush borders. A Bacteroides species was isolated from each sample that secreted protease and destroyed brush border sucrase. We conclude that in bacterial overgrowth syndromes, brush border damage may occur from protease(s) secreted by Bacteroides.

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Section: Resultssupporting

confidence: 56%

Section: Introductionmentioning

confidence: 94%

Effect of Secreted Bacteroides Proteases on Human Intestinal Brush Border Hydrolases

Riepe¹,

Goldstein²,

Alpers³

1980

J. Clin. Invest.

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“…The literature search found 2053 articles overall. After the revision process, reported more in detail in Figure , only 11 articles fulfilled the criteria considered for our analysis …”

Section: Resultsmentioning

confidence: 99%

Small intestinal bacterial overgrowth and celiac disease: A systematic review with pooled‐data analysis

Losurdo

Marra

Shahini

et al. 2017

Neurogastroenterology Motil

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“…Small-intestinal bacterial overgrowth (SIBO) has been suggested as one potentially reversible cause of poorly responsive celiac disease in up to two thirds of patients, although the true prevalence is debated and may actually be much lower, as low as 11% in one study that used jejunal aspirate cultures to diagnose SIBO [Rubio-Tapia et al 2009;Rana et al 2007;Tursi et al 2003;Abdulkarim et al 2002;Prizont et al 1970]. …”

Section: Poorly Responsive Celiac Diseasementioning

confidence: 99%

A review of rifaximin and bacterial overgrowth in poorly responsive celiac disease

Chang

Green

2011

Therap Adv Gastroenterol

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A proportion of patients with celiac disease have a poor response to a gluten-free diet, which may be due to small-intestinal bacterial overgrowth (SIBO). Treatment with rifaximin is often used in the clinical setting, but there is limited literature to support this practice. In addition, challenges in the diagnosis of SIBO confound response interpretation. Our recent placebo-controlled trial did not demonstrate any improvement in gastrointestinal symptoms after treatment with rifaximin and casts doubt on the utility of lactulose-hydrogen breath testing for SIBO in this population.

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Jejunal Bacterial Flora in Chronic Small Bowel Disease

Cited by 36 publications

References 12 publications

Effect of Secreted Bacteroides Proteases on Human Intestinal Brush Border Hydrolases

Effect of Secreted Bacteroides Proteases on Human Intestinal Brush Border Hydrolases

Small intestinal bacterial overgrowth and celiac disease: A systematic review with pooled‐data analysis

A review of rifaximin and bacterial overgrowth in poorly responsive celiac disease

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