JCVI-syn3.0 – A synthetic genome stripped bare!

Sleator, Roy D.

doi:10.1080/21655979.2016.1175847

Cited by 10 publications

(5 citation statements)

References 23 publications

(22 reference statements)

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“…In the longer term, synthetic biology is likely to play a key role in the further development of the field (Sleator 2014b). The creation of the first cell with a synthetic genome (Gibson et al 2010) and more recently the creation of a minimal bacterial genome (Hutchison et al 2016, Sleator 2016) have laid the foundation for the design and creation of whole new bacterial cells, tailored for a specific purpose and with a specific set of genes. In just 20 years, the field has advanced from sequencing the first bacterial genome to the design, synthesis, and assembly of a synthetic genome (Sleator 2016) and associated biomolecules (Sleator 2013b).…”

Section: Discussionmentioning

confidence: 99%

“…The creation of the first cell with a synthetic genome (Gibson et al 2010) and more recently the creation of a minimal bacterial genome (Hutchison et al 2016, Sleator 2016) have laid the foundation for the design and creation of whole new bacterial cells, tailored for a specific purpose and with a specific set of genes. In just 20 years, the field has advanced from sequencing the first bacterial genome to the design, synthesis, and assembly of a synthetic genome (Sleator 2016) and associated biomolecules (Sleator 2013b). Further advances, including automated genetic circuit design (Nielsen et al 2016), whole-cell computational models (Sleator 2012), DNA-mediated big data storage (O'Driscoll & Sleator 2013), and an expanding genetic alphabet (Sleator 2014a), will ultimately lead to the next generation of true designer probiotics.…”

Section: Discussionmentioning

confidence: 99%

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Engineered Probiotics: Applications and Biological Containment

Sola-Oladokun

Culligan

Sleator

2017

Annu. Rev. Food Sci. Technol.

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Bioengineered probiotics represent the next generation of whole cell-mediated biotherapeutics. Advances in synthetic biology, genome engineering, and DNA sequencing and synthesis have enabled scientists to design and develop probiotics with increased stress tolerance and the ability to target specific pathogens and their associated toxins, as well as to mediate targeted delivery of vaccines, drugs, and immunomodulators directly to host cells. Herein, we review the most significant advances in the development of this field. We discuss the critical issue of biological containment and consider the role of synthetic biology in the design and construction of the probiotics of the future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Engineered Probiotics: Applications and Biological Containment

Sola-Oladokun

Culligan

Sleator

2017

Annu. Rev. Food Sci. Technol.

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“…Genome design rules remain poorly understood, and most synthetic genome projects rely on trial-and-error approaches, involving long and fastidious rounds of optimization. For instance, to create JCVI-syn3.0, it took not only many rounds of genome design, transposon mutagenesis, and debugging, but also an extensive knowledge of the biochemical data available in the literature as well as an impressive amount of time and resources (Sleator, 2010;Sleator, 2016;Hutchison et al, 2016). Systems biology approaches that can integrate multiple layers of information and systematically evaluate genome designs represent promising tools in that context (Chalkley et al, 2019;Rees-Garbutt et al, 2020a;Rees-Garbutt et al, 2020b).…”

Section: Is the Genome Of M Florum Minimal?mentioning

confidence: 99%

Mesoplasma florum: a near-minimal model organism for systems and synthetic biology

Matteau,

Duval,

Baby

et al. 2024

Front. Genet.

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Mesoplasma florum is an emerging model organism for systems and synthetic biology due to its small genome (∼800 kb) and fast growth rate. While M. florum was isolated and first described almost 40 years ago, many important aspects of its biology have long remained uncharacterized due to technological limitations, the absence of dedicated molecular tools, and since this bacterial species has not been associated with any disease. However, the publication of the first M. florum genome in 2004 paved the way for a new era of research fueled by the rise of systems and synthetic biology. Some of the most important studies included the characterization and heterologous use of M. florum regulatory elements, the development of the first replicable plasmids, comparative genomics and transposon mutagenesis, whole-genome cloning in yeast, genome transplantation, in-depth characterization of the M. florum cell, as well as the development of a high-quality genome-scale metabolic model. The acquired data, knowledge, and tools will greatly facilitate future genome engineering efforts in M. florum, which could next be exploited to rationally design and create synthetic cells to advance fundamental knowledge or for specific applications.

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“…The new organism was named “JCVI-syn1.0” or more informally “Synthia” ( Powell, 2018 ). In 2016 a new version of the synthetic organism was presented containing only 473 genes, the minimum number required to sustain life under ideal environmental conditions ( Sleator, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Overstatements and Understatements in the Debate on Synthetic Biology, Bioterrorism and Ethics

Melin

2021

Front. Bioeng. Biotechnol.

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Synthetic biology has many valuable applications, but it also gives rise to certain risks. In this paper I discuss the risk of bioterrorism, which often attracts attention in both the mass media and scientific debate, as well as in government reports. While some authors argue that there is a significant risk of bioterrorism connected to synthetic biology, other scholars claim that the risk is exaggerated and that actors often have motives for overstating the risk. In this paper, I argue that some estimates of the risk may be overstated but that certain risks of bioterrorism, such as the creation and spread of known pathogenic viruses, need to be taken seriously. Actors may also have scientific and financial motives for understating the risk. Such understatements are sometimes based on a principle of hope, which says that technological progress is important for the future welfare of humanity and that too much precaution would have bad consequences. I argue that this principle is problematic as the burdens and benefits of synthetic biology may not be equally divided between different social groups. Instead, I claim that the principle of precaution is more justified as a point of departure for assessing advancements within synthetic biology. It tells us that we need strong evidence that such advancements are safe, because there is a potential risk that they may make it easier for terrorist groups to create and spread known pathogenic viruses.

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JCVI-syn3.0 – A synthetic genome stripped bare!

Cited by 10 publications

References 23 publications

Engineered Probiotics: Applications and Biological Containment

Engineered Probiotics: Applications and Biological Containment

Mesoplasma florum: a near-minimal model organism for systems and synthetic biology

Overstatements and Understatements in the Debate on Synthetic Biology, Bioterrorism and Ethics

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