JC virus in the Irish population: Significant increase of genotype 2 in immunocompromised individuals

Schaffer, Kirsten; Sheehy, Noreen; Coughlan, Suzie; Bergin, Colm; Hall, William W.

doi:10.1080/13550280600614965

Cited by 20 publications

(51 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…17 The most frequent JCV subtype was 1b, similar to Mediterranean countries of Europe. 22,23,27 However, subtypes 2b and 3a, also highly prevalent in Brazil, are not common in the north hemisphere. 23 Discrimination of JCV subtypes is useful for studies regarding routes of JCV migration, but there is no evidence that different subtypes have different effects on JCV pathogenesis.…”

Section: Discussionmentioning

confidence: 95%

Molecular characterization of human polyomavirus JC in Brazilian AIDS patients with and without progressive multifocal leukoencephalopathy

Fink

Oliveira

Romano

et al. 2010

Journal of Clinical Virology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 95%

Molecular characterization of human polyomavirus JC in Brazilian AIDS patients with and without progressive multifocal leukoencephalopathy

Fink

Oliveira

Romano

et al. 2010

Journal of Clinical Virology

View full text Add to dashboard Cite

show abstract

“…JCV DnA was detected by amplifying a 215 bp fragment of the VP1 gene in a one step PCR reaction (nucleotides 1710-1734 and 1924-1902) (Agostini et al 1997). The amplification was performed according to Shaffer et al (2006). BKV DnA was detected by amplifying a 354 nucleotide region within the VP1 gene PCR JCV (Agostini et al 1997) PCR BKV (Jin et al 1993) (nt 1663-2016, DUn numbering) using the oligonucleotides BKV-1 (50-GAAGTTCTAGAAGTTAAAACT-GGG-30) and BKV-2 (50-GTGGAAATTACTGCCTT-GAATAGG-30) (Jin et al 1993).…”

Section: Patients Materials and Methodsmentioning

confidence: 99%

Human polyomaviruses JC and BK in the urine of Brazilian children and adolescents vertically infected by HIV

Machado

Fink

Pannuti

et al. 2011

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

The aim of this study was to characterize the urinary excretion of the BK (BKV) and JC (JCV) human polyomaviruses in a cohort of human immunodeficiency virus (HIV)-infected children and adolescents. One hundred and fifty-six patients were enrolled: Group I included 116 HIV-infected children and adolescents [median age = 11.4 years (y); range 1-22 y]; Group II included 40 non-HIV-infected healthy controls (median age = 11.37 y; range 7-16 y). Single urine samples from both groups were screened for the presence of JCV and BKV DNA by polymerase chain reaction at enrolment. The overall rate of JCV and BKV urinary excretion was found to be 24.4% and 40.4%, respectively (n = 156). Group I had urinary excretion of JCV and BKV in 27.6% and 54.3% of subjects, respectively. In contrast, Group II showed positive results for JCV in 17.5% of subjects and for BKV in 12.5% of subjects (p Pearson JCV = 0.20; p Pearson BKV < 0.0001). In Group I, there was no association between JCV/BKV shedding and age, gender or CD4 values. Patients with an HIV viral load < 50 copies/mL had a lower excretion of BKV (p < 0.001) and a trend of lower JCV excretion (p = 0.07). One patient in Group I (1/116, 0.9%) showed clinical and radiological features consistent with progressive multifocal leukoencephalopathy, suggesting that children with HIV/polyomavirus coinfection should be kept under surveillance

show abstract

“…Nested PCR products of two sets of primers used. Column1: control, columns 2–11: nested‐ PCR products of the second round of Nested PCR (primers 13 and 14) (designed in this study), Columns 12–21: Nested‐PCR products of the second Nested PCR (primers 15 and 16) [used by Schaffer et al, ], Column 22: 100 bp DNA Ladder (CinnaGen, Iran).…”

Section: Resultsmentioning

confidence: 99%

“…A set of primers were designed by Oligo 7 software (primers 13–3, 14–4, 13, 14) for genotyping of the virus based on vp1 gene (Mad‐1 prototype virus, accession number: NC_001699). Also a set of already reported primers (primers 15–5, 16–6, 15, 16) [Schaffer et al, ] were also used to confirm the frequency results (Table ).…”

Section: Methodsmentioning

confidence: 99%

“…A volume of 0.5 μl of the first round products were used as template for the second rounds. For Schaffer et al [] primers the conditions were the same expect for the enzyme (Smar Taq DNA polymerase (1.5 U), CinnaGen, Iran and its Buffer). The PCR conditions were as follows: an initial denaturation step of 94°C for 5 min, 40 cycles of 94°C for 30 sec, 60°C for 1 min for first round, while 64°C for 1 min for second round, and 72°C for 1 min, then final extension at 72°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

John cunningham (JC) virus genotypes in kidney transplant recipients, rheumatoid arthritis patients and healthy individuals in Isfahan, Iran

Atyabi

Bouzari

Kardi³

2016

Journal of Medical Virology

View full text Add to dashboard Cite

In healthy individuals John Cunningham virus is latent without any clinical signs, but in the cases of the use of immunosuppressive drugs in graft recipients, autoimmune diseases and also increasing of age, that the immune system is suppressed it may cause disease in reactivation. Progressive multifocal leukoencephalopathy (PML) is the well-known disease caused by the virus. It has also been associated with nephropathy and tumorogensis. At present, based on vp1 capsid gene 7 genotypes have been detected. Genetic variations of JC virus in different geographical areas and the presence of different subtypes is a useful tool for reconstructing of the genetic information of JC virus and understanding of its evolution. The aim of this study was to investigate different genotypes of the JC virus in the urine of 100 kidney transplant recipients, 43 rheumatoid arthritis patients, and 100 healthy individuals as control group in Isfahan. DNA was extracted by phenol-chloroform method and subjected to a nested PCR using specific primer for vp1 capsid gene designed by Oligo 7 software. Fisher's exact test was used for statistical analyses. Using MEGA 6 software the sequences were aligned using Clustal W tool and phylogenetic trees were constructed by neighbor joining method. Thirty-one positive samples were sequenced. Genotypes 1, 3, and 4 of the virus were detected for the first time in Iran. For the first time genotype 3 was reported as the dominant genotype in Iran. For the first time in the world, genotype 4 was detected in rheumatoid arthritis patients. J. Med. Virol. 89:337-344, 2017. © 2016 Wiley Periodicals, Inc.

show abstract

JC virus in the Irish population: Significant increase of genotype 2 in immunocompromised individuals

Cited by 20 publications

References 25 publications

Molecular characterization of human polyomavirus JC in Brazilian AIDS patients with and without progressive multifocal leukoencephalopathy

Molecular characterization of human polyomavirus JC in Brazilian AIDS patients with and without progressive multifocal leukoencephalopathy

Human polyomaviruses JC and BK in the urine of Brazilian children and adolescents vertically infected by HIV

John cunningham (JC) virus genotypes in kidney transplant recipients, rheumatoid arthritis patients and healthy individuals in Isfahan, Iran

Contact Info

Product

Resources

About