Jawbones Scaffold Constructed by TGF-β1 and BMP-2 Loaded Chitosan Microsphere Combining with Alg/HA/ICol for Osteogenic-Induced Differentiation

Abstract

Bone scaffolds based on multi-components are the leading trend to address the multifaceted prerequisites to repair various bone defects. Chitosan is the most useable biopolymer, having excellent biological applications. Therefore, in the present study, the chitosan microsphere was prepared by the ion–gel method; transforming growth factor β (TGF-β1) and bone morphogenetic protein 2 (BMP-2) were loaded onto it and then combined with alginate/hyaluronic acid/collagen (Alg/HA/ICol) to construct a jawbones scaffold. The Alg/HA/ICol scaffolds were characterized by FTIR and SEM, and the water content, porosity, tensile properties, biocompatibility, and osteogenic-induced differentiation ability of the Alg/HA/ICol jawbones scaffolds were studied. The results indicate that a three-dimensional porous jawbone scaffold was successfully constructed having 100–250 μm of pore size and >90% of porosity without cytotoxicity against adipose-derived stem cells (ADSCs). Its ALP quantification, osteocalcin expression, and Von Kossamineralized nodule staining was higher than the control group. The jawbones scaffold constructed by TGF-β1 and BMP-2 loaded chitosan microsphere combining with Alg/HA/ICol has potential biomedical application in the future.

Preparation and biological properties of ZnO/hydroxyapatite/chitosan-polyethylene oxide@gelatin biomimetic composite scaffolds for bone tissue engineering

“…4,5 At present, there are many studies on the preparation of bioscaffold materials using the antibacterial activity of modified chitin or chitosan. [6][7][8][9] Chitosan's structure is similar to the glycosaminoglycans of bone tissue. [10][11][12][13] It has been found that adding polyethylene oxide (PEO) into CS solution could not only improve the electrospinning performance of CS, but also further enhance the hydrophilicity of CS-containing nanofibers.…”

Comparative study of dedifferentiated fat cell and adipose‐derived stromal cell sheets for periodontal tissue regeneration: In vivo and in vitro evidence

“…Synthetic materials such as β‐tricalcium phosphate/collagen‐I fibre scaffolds (Wang et al, 2021), hydrogel scaffolds (Kim et al, 2020), and PCL‐based nanofibrous composite scaffolds (Mansour et al, 2022) have also been employed to stimulate osteogenic differentiation of ADSCs. Bioactive factors such as growth factors, miRNAs, and exosomes have been harnessed to enhance the osteogenic differentiation of ADSCs (S. Yang et al, 2020; Tan et al, 2021). These strategies should be explored to improve periodontal tissue regeneration using DFAT cells in the future.…”

“…to enhance the osteogenic differentiation of ADSCs (S. Yang et al, 2020;Tan et al, 2021). These strategies should be explored to improve periodontal tissue regeneration using DFAT cells in the future.…”

Advances in Barrier Membranes for Guided Bone Regeneration Techniques

“…The combined use of VEGF and rhBMP-2 can increase angiogenesis and blood supply, promote the formation of new bone and solve the problem of vertical bone regeneration in clinical work (Schorn et al, 2017). There is also TGF (Tan et al, 2021) similar to BMP synergistic osteogenesis. In addition to growth factors, some animal and plant active components such as BMP-2-related peptide P28 (Sun T. et al, 2018), amelogenin (EMD) (Miron et al, 2017) and Icariin (ICA) (Yin et al, 2017b) have also been applied in bone tissue engineering.…”