Jatropha curcas seed cake as substrate for production of xylanase and cellulase by Aspergillus niger FGSCA733 in solid-state fermentation

Ncube, Thembekile; Howard, R. L.; Abotsi, E; Rensburg, Elbert Lukas Jansen van; Ncube, Ignatious

doi:10.1016/j.indcrop.2011.11.024

Cited by 89 publications

(46 citation statements)

References 25 publications

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“…Among these cellulase producers, Trichoderma reesei and Aspergillus niger have attracted most attention due to their high cellulase productivity, safe use in industry and the availability of their whole genome sequences. Both submerged fermentation and solid state fermentation (SSF) have been used in cellulase production [9]. In comparison with submerged fermentation, SSF is easy to operate, requires minimum equipment and has the unique advantage of being able to handle insoluble solid substrates, such as lignocellulose.…”

Section: Introductionmentioning

confidence: 99%

Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Abdullah

Greetham

Pensupa

et al. 2016

J Fundam Renewable Energy

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This paper explores the possibility of using an industrially processed municipal solid waste (MSW) for cellulase enzyme production via solid state fermentation (SSF) by Trichoderma reesei and Aspergillus niger. Both fungi grew well on the MSW substrate and production of cellulase enzymes was optimized for temperature, moisture content, inoculation and period of incubation. The effect of additional minerals, and alternative carbon and nitrogen sources were also examined.Following optimization a cellulase activity of 26.10 ± 3.09 FPU/g could be produced using T. reesei at 30°C with a moisture content of 60% with an inoculums of 0.5 million spores/g and incubation for 168 hours. Addition of extra nitrogen and/or carbon did not improve cellulase accumulation. Acid or alkali pretreatment of MSW led to reduced cellulase production. Crude enzymes produced from MSW by T. reesei were evaluated for their ability to release glucose from MSW. A cellulose hydrolysis yield of 24.7% was achieved, which was close to that obtained using a commercial enzyme. Results demonstrated that MSW can be used as an inexpensive lignocellulosic material for the production of cellulase enzymes. Research has revealed that T. reesei can produce 13.4 FPU/g cellulase activity using water hyacinth [8]; 154.58 FPU/g using sugar cane bagasse [10]; 8.2 FPU/g using oil palm empty fruit bunches [11]; and 1.16 FPU/g using rice bran [12] as substrates. Similarly it has been reported that A. Niger could produce 24 FPU/g cellulase activity using wheat straw as a substrate [13]. Other substrates such as banana peel, rice straw, corn cob residue, rice husk, banana fruit stalk, and coconut coir pith have all being used for cellulase production [14,15]. Optimizing Cellulase Production fromAlthough MSW can contain a high lignocellulosic content, there has been limited research into the use of MSW for cellulase production. Other research demonstrated the possibility of using MSW to produce cellulase via SSF. But by using raw not autoclave MSW [16,17]. In this paper, we report an example of using an industrially processed MSW for cellulase production and then the use of the resulting "cellulase cocktail" for the enzymatic hydrolysis of MSW. Materials and Methods Municipal solid wasteMunicipal solid waste (MSW) was kindly supplied by Wilson Steam Storage Ltd (UK). The MSW had been subjected to commercial steam 15 min, respectively. After autoclaving, samples were neutralized to pH 7.0 using either 1 M NaOH or 1 M H 2 SO 4 as appropriate. The samples were then centrifuged at 5000 rpm (4472 g) for 10 min. The solid fraction (pellet) was rinsed three times by adding distilled water to the pellet and re-centrifuged. The solid fraction was then dried overnight at room temperature. Journal of Fundamentals of Renewable Energy and ApplicationsMicroorganisms: Two filamentous fungi, Trichoderma reesei QM6a and Aspergillus niger N402 were kindly donated by Professor David Archer (University of Nottingham, UK). Procedures for storing and cultivating T. reesei and A. n...

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Section: Introductionmentioning

confidence: 99%

Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Abdullah

Greetham

Pensupa

et al. 2016

J Fundam Renewable Energy

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“…On the other hand, Panagiotou et al (2003) reported maximum production of xylanase under the optimum temperature of 27 °C but about 13% dropped at in production at 30 °C. While, Ncube et al (2012) obtained optimum xylanase production at when A. niger was grown on Jatropha curcas seed cake at 25 °C in SSF.…”

Section: Effect Of Different Incubation Temperature On Xylanase Produmentioning

confidence: 94%

Governing parameters on xylanase production using developed bioreactor

Lee¹,

Demirtaş²,

Omar³

2015

MJM

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Aims: Chemicals bleaching are common steps in pulp and paper manufacturing process. The reduction of chemical use would result in lower waste treatment costs while minimizes the impact on the environment. This can be achieved using enzyme (xylanase). Compared to submerged fermentation process, the cost for enzyme production is much cheaper using solid state fermentation process (SSF). The present study aimed to examine factors affect xylanase production using developed SSF bioreactor. Methodology and results:The performance of developed bioreactor namely FERMSOSTAT had been investigated in xylanase production studies. In this studies, local isolate Aspergillus niger USM AI 1 was used together with sugarcane bagasse and palm kernel cake (ratio 1:1), which serve as substrates for xylanase production. The highest xylanase activity and production of 397 U/g and 103 U/mgG glucosamine were obtained, respectively when the fermentation process was carried out under examined enzyme production conditions of 0.5 kg substrate; 70% (w/w) moisture content; 30 °C; aeration at 4 L/h.g fermented substrate for 5 min and mixing at 0.5 rpm for 5 min. On the other hand, compared to initial SSF conditions for xylanase production, xylanase activity increased 681% after all factors affect xylanase production have been examined. Conclusion, significance and impact of study: The performance of the newly developed bioreactor is good since it can be used to produce xylanase with high title when compared with previous researcher findings.

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“…A preparação do inóculo ocorreu pela adição de 20 mL de solução de Tween 80 0,2% à placa contendo os esporos, sendo transferidos para a solução com o auxílio de alça de Drigalski. Em seguida determinou-se a concentração de esporos (câmara de Neubauer), sendo inoculado no meio de fermentação 1 mL desta suspensão na concentração de 1 × 10 6 esporos/ mL (Ncube et al, 2012).…”

Section: Preparo Do Inóculounclassified

“…Esta aplicação tem como principais motivadores a abundância destes materiais, ricos em nutrientes, além de não possuírem custos diretos de produção, contribuindo para a sustentabilidade dos sistemas de produção (Ncube et al, 2012).…”

Section: Introductionunclassified

ESTUDO DA INFLUÊNCIA DO pH E DA TEMPERATURA NA PRODUÇÃO DE CELULASES POR Mucor circinelloides

Maciel¹,

Paixão²,

Oliveira³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -As celulases são enzimas que têm se destacado no mundo científico em virtude do interesse na produção do etanol de segunda geração. Desta forma, diversas pesquisas têm se concentrado no desenvolvimento de novos bioprocessos para a produção de celulases microbianas mais eficientes e estáveis que as disponíveis atualmente. Neste contexto, o objetivo do presente trabalho foi avaliar o efeito do pH e da temperatura na produção de celulases a partir de Mucor circinelloides, um micro-organismo recentemente isolado por nosso grupo de pesquisa, sendo utilizado bagaço de cana-de-açúcar como substrato para a fermentação em estado sólido. Diferentes combinações de pH e temperatura foram estudadas através de um planejamento fatorial composto central (22). Os resultados observados a partir da superfície de resposta, mostraram um ponto de máximo, pH 5,48 e 34,4 ºC, no qual a atividade enzimática foi igual a 6,00 ± 0,32 FPU/g.

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Jatropha curcas seed cake as substrate for production of xylanase and cellulase by Aspergillus niger FGSCA733 in solid-state fermentation

Cited by 89 publications

References 25 publications

Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Optimizing Cellulase Production from Municipal Solid Waste (MSW) using Solid State Fermentation (SSF)

Governing parameters on xylanase production using developed bioreactor

ESTUDO DA INFLUÊNCIA DO pH E DA TEMPERATURA NA PRODUÇÃO DE CELULASES POR Mucor circinelloides

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