Jasmonoyl-<scp>l</scp>-isoleucine is required for the production of a flavonoid phytoalexin but not diterpenoid phytoalexins in ultraviolet-irradiated rice leaves

Miyamoto, Koji; Enda, Isami; Okada, Toshiki; Sato, Yumiko; Watanabe, Kohei; Sakazawa, Tomoko; Yumoto, Emi; Shibata, Kyomi; Asahina, Masashi; Iino, Masashi; Yokota, Takao; Okada, Kazunori; Yamane, Hisakazu

doi:10.1080/09168451.2016.1189319

Cited by 24 publications

(22 citation statements)

References 27 publications

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“…Root samples were harvested 1–12 mm from the root–shoot junction at 0, 2 and 8 h after root cutting, and frozen in liquid nitrogen. The hormone analysis was carried out as described previously ( Miyamoto et al 2016 , Enomoto et al 2017 ). Briefly, samples of approximately 100 mg FW were suspended in 80% (v/v) aqueous methanol with [ 13 C 6 ]IAA, [ 2 H 2 ]JA and [ 13 C 6 ]JA-Ile as internal standards.…”

Section: Methodsmentioning

confidence: 99%

YUCCA9-Mediated Auxin Biosynthesis and Polar Auxin Transport Synergistically Regulate Regeneration of Root Systems Following Root Cutting

Miao

Yumoto

et al. 2017

Plant and Cell Physiology

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Recovery of the root system following physical damage is an essential issue for plant survival. An injured root system is able to regenerate by increases in lateral root (LR) number and acceleration of root growth. The horticultural technique of root pruning (root cutting) is an application of this response and is a common garden technique for controlling plant growth. Although root pruning is widely used, the molecular mechanisms underlying the subsequent changes in the root system are poorly understood. In this study, root pruning was employed as a model system to study the molecular mechanisms of root system regeneration. Notably, LR defects in wild-type plants treated with inhibitors of polar auxin transport (PAT) or in the auxin signaling mutant auxin/indole-3-acetic acid19/massugu2 were recovered by root pruning. Induction of IAA19 following root pruning indicates an enhancement of auxin signaling by root pruning. Endogenous levels of IAA increased after root pruning, and YUCCA9 was identified as the primary gene responsible. PAT-related genes were induced after root pruning, and the YUCCA inhibitor yucasin suppressed root regeneration in PAT-related mutants. Therefore, we demonstrate the crucial role of YUCCA9, along with other redundant YUCCA family genes, in the enhancement of auxin biosynthesis following root pruning. This further enhances auxin transport and activates downstream auxin signaling genes, and thus increases LR number.

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Section: Methodsmentioning

confidence: 99%

YUCCA9-Mediated Auxin Biosynthesis and Polar Auxin Transport Synergistically Regulate Regeneration of Root Systems Following Root Cutting

Miao

Yumoto

et al. 2017

Plant and Cell Physiology

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“…The elicitation was considered to arise from the induction of naringenin 7- O -methyltransferase, a key enzyme in sakuranetin biosynthesis [156]. Very interestingly, JA-Ile was not required for diterpenoid production in blast pathogen-infected or CuCl 2 -treated rice leaves [157].…”

Section: Metabolic Regulation Of Secondary Meatobolitesmentioning

confidence: 99%

Rice Secondary Metabolites: Structures, Roles, Biosynthesis, and Metabolic Regulation

Wang

Dang

et al. 2018

Molecules

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Rice (Oryza sativa L.) is an important food crop providing energy and nutrients for more than half of the world population. It produces vast amounts of secondary metabolites. At least 276 secondary metabolites from rice have been identified in the past 50 years. They mainly include phenolic acids, flavonoids, terpenoids, steroids, alkaloids, and their derivatives. These metabolites exhibit many physiological functions, such as regulatory effects on rice growth and development, disease-resistance promotion, anti-insect activity, and allelopathic effects, as well as various kinds of biological activities such as antimicrobial, antioxidant, cytotoxic, and anti-inflammatory properties. This review focuses on our knowledge of the structures, biological functions and activities, biosynthesis, and metabolic regulation of rice secondary metabolites. Some considerations about cheminformatics, metabolomics, genetic transformation, production, and applications related to the secondary metabolites from rice are also discussed.

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“…In the final step of sakuranetin biosynthesis, the precursor naringenin is converted to sakuranetin by naringenin 7- O -methyltransferase (NOMT), which is encoded by OsNOMT 11. We recently reported that OsNOMT expression is induced by JA treatment12, and jasmonoyl- l -isoleucine (JA-Ile), an active form of jasmonate, is required for the production of sakuranetin10. We have previously shown that CuCl 2 is a potent elicitor to induce sakuranetin production in wild-type plants, while such CuCl 2 -inductive production of sakuranetin is specifically compromised in a JA-Ile deficient mutant osjar1 10.…”

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confidence: 99%

OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability

Ogawa

Miyamoto

Nemoto

et al. 2017

Sci Rep

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Biosynthesis of sakuranetin, a flavonoid anti-fungal phytoalexin that occurs in rice, is highly dependent on jasmonic acid (JA) signalling and induced by a variety of environmental stimuli. We previously identified OsNOMT, which encodes naringenin 7-O-methyltransferase (NOMT); NOMT is a key enzyme for sakuranetin production. Although OsNOMT expression is induced by JA treatment, the regulation mechanism that activates the biosynthetic pathway of sakuranetin has not yet been elucidated. In this study, we show that JA-inducible basic helix-loop-helix transcriptional factor OsMYC2 drastically enhances the activity of the OsNOMT promoter and is essential for JA-inducible sakuranetin production. In addition, we identified 2 collaborators of OsMYC2, OsMYC2-like protein 1 and 2 (OsMYL1 and OsMYL2) that further activated the OsNOMT promoter in synergy with OsMYC2. Physical interaction of OsMYC2 with OsMYL1 and OsMYL2 further supported the idea that these interactions lead to the enhancement of the transactivation activity of OsMYC2. Our results indicate that JA signalling via OsMYC2 is reinforced by OsMYL1 and OsMYL2, resulting in the inductive production of sakuranetin during defence responses in rice.

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Jasmonoyl-l-isoleucine is required for the production of a flavonoid phytoalexin but not diterpenoid phytoalexins in ultraviolet-irradiated rice leaves

Cited by 24 publications

References 27 publications

YUCCA9-Mediated Auxin Biosynthesis and Polar Auxin Transport Synergistically Regulate Regeneration of Root Systems Following Root Cutting

YUCCA9-Mediated Auxin Biosynthesis and Polar Auxin Transport Synergistically Regulate Regeneration of Root Systems Following Root Cutting

Rice Secondary Metabolites: Structures, Roles, Biosynthesis, and Metabolic Regulation

OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability

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