Jasmonic acid regulates the biosynthesis of medicinal metabolites via the JAZ9-MYB76 complex inSalvia miltiorrhiza

Abstract

Jasmonic acid (JA) signaling pathway plays an important role in tanshinone and phenolic acid biosynthesis in Salvia miltiorrhiza. However, the specific regulatory mechanism remains largely unclear. Previous work showed that a JASMONATE ZIM-domain (JAZ), SmJAZ9, acted as a repressor of tanshinone production in S. miltiorrhiza. In this study, we revealed that SmJAZ9 reduced both phenolic acid accumulation and related biosynthetic gene expression, confirming that SmJAZ9 also negatively affected phenolic acid biosynthesis. Then, we identified a novel MYB transcription factor,SmMYB76, which interacted with SmJAZ9. SmMYB76 repressed phenolic acid biosynthesis by directly downregulating SmPAL1, Sm4CL2, and SmRAS1. Further investigation demonstrated that JA mediated phenolic acids biosynthesis via SmJAZ9-SmMYB76 complex. Taken together, these findings state the molecular mechanism that SmJAZ9-SmMYB76 regulated phenolic acid biosynthesis at the transcriptional and protein levels, which provided new insights into JA signaling pathway regulating plant metabolism.

Transcriptome sequencing and metabolome analysis reveal the molecular mechanism of Salvia miltiorrhiza in response to drought stress

“…Moreover, drought stress could also stimulate ABA accumulation. For example, in P. nutans , PYL, PP2C, and SnRK2 were notably upregulated [ 64 ]. DEGs that encode PYL, PP2C, and SnRK2 in our research, however, showed a decrease or no significant change.…”

“…TFs are crucial for the response to drought stress and the regulation of secondary metabolites [ 64 , 65 ]. Previous results have proven that some TFs function through the MAPK signaling pathway and plant hormone signal transduction.…”

Genome-Wide Identification and Characterization of the Salvia miltiorrhiza Histone Deacetylase (HDAC) Family in Response to Multiple Abiotic Stresses

“…It is worth noting that SmHDA5 also positively correlated with the expression of SmJAZ9 . In previous reports, SmJAZ9 was shown that it can form the complex with SmMYB76 , further inhibit the expression of the synthetic gene SmRAS1 , SmMYB76 was also associated with SmHDA5 [ 2 ]. The addition of SmHDA5 may provide a new idea for the molecular mechanism of SmMYB76 inhibiting salvianolic acid synthesis.…”

“…Salvia miltiorrhiza is a Chinese traditional medicinal plant that has been used for thousands of years. It is rich in water-soluble component salvianolic acids and lipid-soluble component tanshinones [ 1 , 2 ], which is used to combat the increasing incidence of cardiovascular disease [ 3 , 4 ]. However, the wild resources of S. miltiorrhiza are decreasing continuously, which cannot meet the market demand.…”

“…For example, JA signaling pathway induced by jasmonic acid (JA) has been proved to significantly increase the content of salvianolic acid [ 9 ]. In-depth study on JA pathway found that some transcription factors activated by JA pathway can enhance the transcription of salvianolic acid key enzyme genes, such as basic helix-loop-helix (bHLH), R2R3-MYB transcription factor MYB76, MYB1, MYB2 [ 2 , 10 , 11 ]; APETALA2/ERF (AP2/ERF) transcription factor ERF115 [ 12 ]. However, in addition to transcription factors, there are many other factors affecting gene expression such as post-translational modifications (PTMs).…”