Jasmonates induce over-accumulation of methylputrescine and conjugated polyamines in Hyoscyamus muticus L. root cultures

Biondi, Stefania; Fornalé, Silvia; Oksman-Caldentey, Kirsi-Marja; Eeva, Manu; Agostani, S.; Bagni, Nello

doi:10.1007/s002999900178

Cited by 67 publications

(27 citation statements)

References 34 publications

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“…Although PMT expression was reported to be induced by jasmonate treatment in two tropane alkaloid-producing species, Anisodus acutangulus (Kai et al 2008) and Hyoscyamus niger (Zhang et al 2002), jasmonate treatment was ine ective in increasing PMT expression in A. belladonna (Suzuki et al 1999b). Formation of hyoscyamine and scopolamine either increased by jasmonate treatment in H. niger (Zhang et al 2002) or did not in A. belladonna (Suzuki et al 1999b), Datura wrightii (Hare and Walling 2006), and H. muticus (Biondi et al 2000). We did not observe reproducible induction of other genes involved in alkaloid biosynthesis (e.g., TR-I and H6H) to be induced by such treatment in A. belladonna and H. niger (our unpublished results).…”

Section: Discussionmentioning

confidence: 66%

DNA-binding and transcriptional activation properties of tobacco <i>NIC2</i>-locus ERF189 and related transcription factors

Shoji

Hashimoto

2012

Plant Biotechnology

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In tobacco (Nicotiana tabacum), several transcription factors belonging to the IXa group of APETALA2/ ETHYLENE RESPONSE FACTOR (AP2/ERF) cluster at the regulatory NIC2 locus for the leaf nicotine level, and directly activate structural genes required for nicotine biosynthesis and transport. Solanaceous Atropa and Hyoscyamus plants synthesize medicinal tropane alkaloids by utilizing enzymes that partially overlap with the nicotine pathway. We tested whether the tobacco NIC2-locus ERF189 binds to potential ERF binding sites in the promoters of various structural genes involved in biosynthesis of nicotine and tropane alkaloids by an electrophoresis mobility shi assay. ERF189 bound four new GCC-box-like sequences in three nicotine structural genes, but did not recognize other candidate binding sites. is binding preference was used to optimize the consensus binding sequence of ERF189 as 5′-(A/C)GC(A/C)NNCC(A/T)-3′. Five group IXa ERFs (tobacco ERF189, tobacco ERF163, Catharanthus ORCA3, Arabidopsis AtERF13, and Arabidopsis AtERF1) were examined whether they bind to the ERF189-recognition site in the promoter of the tobacco putrescine Nmethyltransferase gene in vitro, and transactivate the promoter in a transient expression assay using cultured tobacco cells.e more the protein sequences were similar to ERF189, the more e ective these ERFs were in these functional assays. e transient expression assay also identi ed transactivation domains in an N-terminal acidic region of ERF189, and in a C-terminal Ser-rich region of AtERF13.

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Section: Discussionmentioning

confidence: 66%

DNA-binding and transcriptional activation properties of tobacco <i>NIC2</i>-locus ERF189 and related transcription factors

Shoji

Hashimoto

2012

Plant Biotechnology

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“…Jasmonates, a group of growth regulators produced by plants exposed to stress and under senescence (Koda, 1997;Biondi et al, 2000), are highly effective inducers of tuberization (Van den Berg & Ewing, 1991;Pelacho & Mingo-Castel, 1991;Ravinkar et al, 1992). We found that JA conditioning of stock plants destined for producing explants for in vitro tuberization, or addition of JA to the tuberization medium, enhanced tuber size and uniformity (Pruski et al, 2002).…”

Section: Introductionmentioning

confidence: 88%

Jasmonate effect on in vitro tuberization of potato (Solanum tuberosum L.) cultivars under light and dark conditions

Pruski¹,

Duplessis²,

Lewis³

et al. 2001

Potato Res

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“…39 Jasmonates have been shown to induce over-accumulation of methylputrescine and conjugated PAs in Hyoscyamus muticus L. root cultures. 40 Jasmonic acid and its precursor 12-oxophytodienoic acid increased in hop nodules after 45 days of culture. 41 This increase in jasmonic acid may thus influence the relative accumulation of conjugated PAs …”

Section: Discussionmentioning

confidence: 97%

Arginine Decarboxylase expression, polyamines biosynthesis and reactive oxygen species during organogenic nodule formation in hop

Fortes¹,

Costa²,

Santos

et al. 2011

Plant Signaling & Behavior

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Jasmonates induce over-accumulation of methylputrescine and conjugated polyamines in Hyoscyamus muticus L. root cultures

Cited by 67 publications

References 34 publications

DNA-binding and transcriptional activation properties of tobacco <i>NIC2</i>-locus ERF189 and related transcription factors

DNA-binding and transcriptional activation properties of tobacco <i>NIC2</i>-locus ERF189 and related transcription factors

Jasmonate effect on in vitro tuberization of potato (Solanum tuberosum L.) cultivars under light and dark conditions

Arginine Decarboxylase expression, polyamines biosynthesis and reactive oxygen species during organogenic nodule formation in hop

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