Jasmonate-responsive MYB factors spatially repress rutin biosynthesis in Fagopyrum tataricum

Zhang, Kaixuan; Logacheva, Maria D.; Meng, Yu; Hu, Jianping; Wan, Dongpu; Li, Long; Janovská, Dagmar; Wang, Zhiyong; Georgiev, Milen I.; Zhuo, Yu; Yang, Fan; Yan, Mingli; Zhou, Meiliang

doi:10.1093/jxb/ery032

Cited by 85 publications

(73 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FtMYB116 is responsive to changes in light quality, but FtMYB9, FtMYB10, FtMYB12, FtMYB17, FtMYB21, and FtMYB22 respond to abiotic stresses such as high salinity, drought stress, and cold (Gao, Yao, et al, ; Gao, Zhao, et al, ; Gao et al, ; Zhou et al, ). Moreover, FtMYB116 acts as a positive regulator, but FtMYB11, FtMYB13, FtMYB14, FtMYB15, and FtMYB16 act as negative regulators of flavonoid biosynthesis (K. Zhang, Logacheva, et al, ; Zhou et al, ).…”

Section: Discussionmentioning

confidence: 99%

The light‐induced transcription factor FtMYB116 promotes accumulation of rutin in Fagopyrum tataricum

Zhang

Jiang

Huang

et al. 2018

Plant Cell & Environment

View full text Add to dashboard Cite

Tartary buckwheat (Fagopyrum tataricum) not only provides a supplement to primary grain crops in China but also has high medicinal value, by virtue of its rich content of flavonoids possessing antioxidant, anti‐inflammatory, and anticancer properties. Light is an important environmental factor that can regulate the synthesis of plant secondary metabolites. In this study, we treated tartary buckwheat seedlings with different wavelengths of light and found that red and blue light could increase the content of flavonoids and the expression of genes involved in flavonoid synthesis pathways. Through coexpression analysis, we identified a new MYB transcription factor (FtMYB116) that can be induced by red and blue light. Yeast one‐hybrid assays and an electrophoretic mobility shift assay showed that FtMYB116 binds directly to the promoter region of flavonoid‐3′‐hydroxylase (F3'H), and a transient luciferase activity assay indicated that FtMYB116 can induce F3'H expression. After transforming FtMYB116 into the hairy roots of tartary buckwheat, we observed significant increases in the content of rutin and quercetin. Collectively, our results indicate that red and blue light promote an increase in flavonoid content in tartary buckwheat seedlings; we also identified a new MYB transcription factor, FtMYB116, that promotes the accumulation of rutin via direct activation of F3′H expression.

show abstract

Section: Discussionmentioning

confidence: 99%

The light‐induced transcription factor FtMYB116 promotes accumulation of rutin in Fagopyrum tataricum

Zhang

Jiang

Huang

et al. 2018

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…SENSITIVE TO ABA AND DROUGHT 2 (SAD2), an importin b-like protein, mediates nuclear trafficking of AtMYB4, AtMYB7, and AtMYB32, thereby increasing their repression of their target genes [41,42]. The interaction between SAD2 and members of subgroup 4 occurs through their SID domain GXXDFxxxG/DL, which is also a signature for protein degradation through the 26S proteasome pathway [43]. In buckwheat (Fagopyrum tataricum), jasmonates induce not only the expression of SAD2 and of five members of subgroup 4, but also the degradation of the corresponding proteins through the 26S proteasome pathway [23,43].…”

Section: R2r3 Mybs the Gatekeepers Of Scw Formation And Lignificationmentioning

confidence: 99%

“…The interaction between SAD2 and members of subgroup 4 occurs through their SID domain GXXDFxxxG/DL, which is also a signature for protein degradation through the 26S proteasome pathway [43]. In buckwheat (Fagopyrum tataricum), jasmonates induce not only the expression of SAD2 and of five members of subgroup 4, but also the degradation of the corresponding proteins through the 26S proteasome pathway [23,43]. The subgroup 4 FtMYB11 binds to the AATAGTT motif to repress the expression of FtPAL, FtC4H, and Ft4CL ( Figure 2 and Table 1).…”

Section: R2r3 Mybs the Gatekeepers Of Scw Formation And Lignificationmentioning

confidence: 99%

A Molecular Blueprint of Lignin Repression

Behr¹,

Guerriero²,

Grima‐Pettenati³

et al. 2019

Trends in Plant Science

View full text Add to dashboard Cite

Although lignin is essential to ensure the correct growth and development of land plants, it may be an obstacle to the production of lignocellulosics-based biofuels, and reduces the nutritional quality of crops used for human consumption or livestock feed. The need to tailor the lignocellulosic biomass for more efficient biofuel production or for improved plant digestibility has fostered considerable advances in our understanding of the lignin biosynthetic pathway and its regulation. Most of the described regulators are transcriptional activators of lignin biosynthesis, but considerably less attention has been devoted to the repressors of this pathway. We provide a comprehensive overview of the molecular factors that negatively impact on the lignification process at both the transcriptional and post-transcriptional levels. Challenging LigninLignin is a major cell wall component that fulfills fundamental functions in plant development as well as in defense against pests and pathogens. This heteropolymer impregnates the compound middle lamella and the secondary cell walls (SCWs) of cells genetically programmed to be lignified, such as xylem tracheary elements as well as xylem and phloem fibers. Lignin biosynthesis spans the phenylpropanoid and the monolignol pathways, from phenylalanine to p-coumaryl, coniferyl, and sinapyl alcohols. Monolignol homeostasis is regulated through a balance between the molecular factors that antagonistically regulate their biosynthesis. After monolignols are excreted into the apoplast, they are oxidized by the phenol-oxidoreductase laccases and/or by class III peroxidases, and are polymerized by radical coupling. A complex regulatory network involving phytohormones, transcription factors (TFs), and post-transcriptional events guide SCW deposition and lignification [1]. This network prevents lignin deposition in tissues undergoing active division or elongation such as apical meristems, vascular cambium, and immature xylem cells, as well as in non-lignified tissues such as stem pith and flax or hemp bast fibers that harbor gelatinous walls under normal developmental conditions. Similarly, this network negatively regulates lignification in xylem tissue formed in response to mechanical stresses such as, for instance, poplar tension wood that harbors hypolignified gelatinous walls.

show abstract

“…Tartary buckwheat (Fagopyrum tataricum) is well known to enrich in rutin among other buckwheat species (Figure 1a; Sytar et al, 2014). The subgroup 4 of R2R3-MYB transcription factors (TFs), FtMYB11/13/14/15/16, are key repressors of rutin biosynthesis in buckwheat (Matsui et al, 2004;Zhang et al, 2018;Zhou et al, 2017). However, FtMYB16 is lack of the GY/FDFLGL motif (SID motif) and specially acts as a repressor on rutin biosynthesis in root (Zhang et al, 2018;Zhou et al, 2015).…”

mentioning

confidence: 99%

“…The subgroup 4 of R2R3-MYB transcription factors (TFs), FtMYB11/13/14/15/16, are key repressors of rutin biosynthesis in buckwheat (Matsui et al, 2004;Zhang et al, 2018;Zhou et al, 2017). However, FtMYB16 is lack of the GY/FDFLGL motif (SID motif) and specially acts as a repressor on rutin biosynthesis in root (Zhang et al, 2018;Zhou et al, 2015). The SID motif contributes to the protein interaction with an importinb-like protein that mediates the MYB nuclear trafficking (Zhao et al, 2007;Zhou et al, 2017).…”

mentioning

confidence: 99%

FtMYB16 interacts with Ftimportin‐α1 to regulate rutin biosynthesis in tartary buckwheat

Zhang

Meng

et al. 2019

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

Jasmonate-responsive MYB factors spatially repress rutin biosynthesis in Fagopyrum tataricum

Abstract: Jasmonate-responsive repressors of FtMYBs can be degraded by the 26S proteasome in a COI1-dependent manner in Fagopyrum tataricum and act together with FtSAD2 or FtJAZ1 in the phenylpropanoid pathway.

Cited by 85 publications

References 43 publications

The light‐induced transcription factor FtMYB116 promotes accumulation of rutin in Fagopyrum tataricum

The light‐induced transcription factor FtMYB116 promotes accumulation of rutin in Fagopyrum tataricum

A Molecular Blueprint of Lignin Repression

FtMYB16 interacts with Ftimportin‐α1 to regulate rutin biosynthesis in tartary buckwheat

Contact Info

Product

Resources

About