Jasmonate promotes artemisinin biosynthesis by activating the TCP14-ORA complex in
            <i>Artemisia annua</i>

Ma, Yong; Li, Ling; Zhang, Fei; Fu, Xueqing; Shen, Qian; Lyu, Xueying; Wu, Zhangkuanyu; Pan, Qin; Pu, Shi; Hao, Xiaolong; Yan, Tingxiang; Chen, Ming‐Hui; Pin, Liu; He, Qian; Xie, Lihui; Zhong, Yijun; Zhao, Jingya; Zhang, Lida; Sun, Xiaofen

doi:10.1126/sciadv.aas9357

Cited by 112 publications

(161 citation statements)

References 54 publications

(147 reference statements)

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“…For MeJA treatment, seeds of A . annua were germinated in pots and seedlings were grown in a growth chamber under the growth conditions described previously . For collection of different tissues (root, stem, leaf, shoot, bud, and flower), A. annua plants were grown in a greenhouse for 5 months .…”

Section: Methodsmentioning

confidence: 99%

“…annua were germinated in pots and seedlings were grown in a growth chamber under the growth conditions described previously . For collection of different tissues (root, stem, leaf, shoot, bud, and flower), A. annua plants were grown in a greenhouse for 5 months . For in vitro culture of seedlings used for generation of transgenic plants, A. annua seeds were sown on Murashige and Skoog medium (Sigma–Aldrich, St. Louis, MO, USA) with 3% sucrose and 0.6% agar (pH 5.7) after surface sterilization.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, farnesyl diphosphate is synthesized from two molecules of isopentenyl diphosphate and one molecule dimethylallyl diphosphate, which are produced from the MVA pathway in the cytosol and the MEP pathway in plastids. Farnesyl diphosphate is converted to dihydroartemisinic acid by four trichome‐specific enzymes (amorpha‐4,11‐diene synthase [AaADS], cytochrome P450‐dependent hydroxylase [AaCYP71AV1], double bond reductase 2 [AaDBR2], and aldehyde dehydrogenase 1 [AaALDH1]) and subsequently modified to artemisinin in the trichome subcuticular space .…”

Section: Introductionmentioning

confidence: 99%

“…JAZs are key regulators that act as repressors in JA signaling processes and interact with transcriptional activators to inhibit their transcriptional activation activities . There are nine JAZ proteins in A. annua , and it was revealed that they can repress the transcription of AaMYC2, AaORA, and AaTCP14, which positively regulate artemisinin biosynthesis .…”

Section: Introductionmentioning

confidence: 99%

“…In addition, overexpression of AaMYC2 in A. annua significantly activated the transcription of AaCYP71AV1 and AaDBR2 , and resulted in an increase of artemisinin content . Recently, JA activation of the AaTCP14–AaORA complex was found to activate the AaDBR2 promoter, which subsequently promotes JA‐induced artemisinin accumulation .…”

Section: Introductionmentioning

confidence: 99%

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Jasmonic acid‐responsive AabHLH1 positively regulates artemisinin biosynthesis in Artemisia annua

Li¹,

Hao²,

Liu³

et al. 2019

Biotech and App Biochem

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Artemisia annua is the only natural source of the sesquiterpenoid artemisinin, which is widely used to treat malaria. The phytohormone jasmonic acid (JA) can significantly promote artemisinin biosynthesis in A. annua. AabHLH1 can bind and activate artemisinin biosynthetic genes, such as AaADS and AaCYP71AV1. In this study, we proved that AabHLH1 was responsive to MeJA treatment and highly expressed in glandular trichome-enriched tissues, and that its expression profile was similar to that of AaADS. Yeast two-hybrid assays showed that AabHLH1 interacted with all nine AaJAZ proteins in A. annua. Functional analysis with transgenic plants showed that several artemisinin biosynthetic genes were upregulated in AabHLH1-OE transgenic A. annua lines and downregulated in AabHLH1-EAR lines; furthermore, the artemisinin content was increased in the AabHLH1-OE lines and decreased in the AabHLH1-EAR lines. These results demonstrate that the JA-induced AabHLH1 positively regulates artemisinin biosynthesis by regulating the biosynthetic genes, and thus provide new insight into the regulatory mechanism of JA-induced artemisinin biosynthesis in

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Jasmonic acid‐responsive AabHLH1 positively regulates artemisinin biosynthesis in Artemisia annua

Li¹,

Hao²,

Liu³

et al. 2019

Biotech and App Biochem

Self Cite

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Effects of exogenous indole‐3‐acetic acid on the density of trichomes, expression of artemisinin biosynthetic genes, and artemisinin biosynthesis in Artemisia annua

Guo

Zhang

et al. 2023

Biotech and App Biochem

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Artemisinin is the most practical medication for the treatment of malaria, but is only very minimally synthesized in Artemisia annua, significantly less than the market needs. In this study, indole‐3‐acetic acid (IAA) was used to investigate its effects on trichomes, artemisinin accumulation, and biosynthetic gene expression in A. anuua. The results showed that exogenous IAA could contribute to the growth and development of A. annua and increase the density of trichomes. Analysis using liquid chromatography‐tandem mass spectrometry (LC‒MS/MS) indicated that artemisinin and dihydroartemisinic acid (DHAA) contents were increased by 1.9‐fold (1.1 mg/g) and 2.1‐fold (0.51 mg/g) after IAA treatment in comparison with control lines (CK), respectively. Furthermore, quantitative real‐time PCR results showed that AaADS, AaCYP71AV1, AaALDH1, and AaDBR2, four critical enzyme genes for the biosynthesis of artemisinin, had relatively high transcription levels in leaves of A. annua treated with IAA. In summary, this study indicated that exogenous IAA treatment was a feasible strategy to enhance artemisinin production, which paves the way for further metabolic engineering of artemisinin biosynthesis.

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Accumulation of Secondary Metabolites and Improved Size of Glandular Trichomes in Artemisia annua

Pandey

Tiwari

Kumar

et al. 2019

Reference Series in Phytochemistry

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Glandular trichomes are multicellular epidermal outgrowths that have characteristic globular head made up of secretory cells and store large quantities of specialized secondary metabolites. Artemisia annua is known for its medicinally important secondary metabolite "artemisinin" which is synthesized and stored in glandular trichomes. However, our understanding of morphological and transcriptional control related to glandular trichome development and accumulation of secondary metabolites in A. annua is available in scattered form. This chapter deals with the trichome biology including developmental and functional aspects along with their correlation with secondary metabolite accumulation in response to various biotic and abiotic signals of the environment using A. annua as model. This chapter also emphasizes the molecular mechanisms behind trichome development in A. annua and provides a glimpse of molecular players involved in this process. There are many environmental as well as intrinsic factors which directly or indirectly affect secondary metabolite synthesis and as a result determine the size of glandular trichomes. The compiled information available for A. annua trichome biology can further be utilized for exploring trichome engineering in many medicinal or aromatic plants which are less explored.

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Jasmonate promotes artemisinin biosynthesis by activating the TCP14-ORA complex in Artemisia annua

Abstract: JA-induced JAZ8 degradation releases and activates the TCP14-ORA complex in enhancing artemisinin biosynthesis in Artemisia annua.

Cited by 112 publications

References 54 publications

Jasmonic acid‐responsive AabHLH1 positively regulates artemisinin biosynthesis in Artemisia annua

Jasmonic acid‐responsive AabHLH1 positively regulates artemisinin biosynthesis in Artemisia annua

Effects of exogenous indole‐3‐acetic acid on the density of trichomes, expression of artemisinin biosynthetic genes, and artemisinin biosynthesis in Artemisia annua

Accumulation of Secondary Metabolites and Improved Size of Glandular Trichomes in Artemisia annua

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