Jasmonate-mediated wound signalling promotes plant regeneration

Zhang, Guifang; Zhao, Fei; Chen, Lyuqin; Pan, Yu; Sun, Lijun; Bao, Ning; Zhang, Teng; Cui, Chongwei; Qiu, Zaozao; Zhang, Yijing; Yang, Li; Xu, Lin

doi:10.1038/s41477-019-0408-x

Cited by 204 publications

(226 citation statements)

References 44 publications

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“…In our study, two genes of ERF109 showed a −6.43 and −6.50 FC of down-regulation in the LD group. Zhang et al also uncovered the activation of ERF109 by JA, which further upregulates the ANTHRANILATE SYNTHASE α1 (ASA1)-a tryptophan biosynthesis gene in the auxin production pathway, promoting the de novo regeneration of root [42]. Therefore, the coordination between wound response or wound-induced immunity and regeneration might be built through JA signaling.…”

Section: Discussionmentioning

confidence: 99%

Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.

Dong

Li-zhi

et al. 2019

Genes

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De novo shoot regeneration is one of the important manifestations of cell totipotency in organogenesis, which reflects a survival strategy organism evolved when facing natural selection. Compared with tissue regeneration, and somatic embryogenesis, de novo shoot regeneration denotes a shoot regeneration process directly from detatched or injured tissues of plant. Studies on plant shoot regeneration had identified key genes mediating shoot regeneration. However, knowledge was derived from Arabidopsis; the regeneration capacity is hugely distinct among species. To achieve a comprehensive understanding of the shoot regeneration mechanism from tree species, we select four genetic lines of Populus euphratica from a natural population to be sequenced at transcriptome level. On the basis of the large difference of differentiation capacity, between the highly differentiated (HD) and low differentiated (LD) groups, the analysis of differential expression identified 4920 differentially expressed genes (DEGs), which were revealed in five groups of expression patterns by clustering analysis. Enrichment showed crucial pathways involved in regulation of regeneration difference, including "plant hormone signal transduction", "cell differentiation", "cellular response to auxin stimulus", and "auxin-activated signaling pathway". The expression of nine genes reported to be associated with shoot regeneration was validated using quantitative real-time PCR (qRT-PCR). For the specificity of regeneration mechanism with P. euphratica, large amount of DEGs involved in "plant-pathogen interaction", ubiquitin-26S proteosome mediated proteolysis pathway, stress-responsive DEGs, and senescence-associated DEGs were summarized to possibly account for the differentiation difference with distinct genotypes of P. euphratica. The result in this study helps screening of key regulators in mediating the shoot differentiation. The transcriptomic characteristic in P. euphratica further enhances our understanding of key processes affecting the regeneration capacity of de novo shoots among distinct species.

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Section: Discussionmentioning

confidence: 99%

Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.

Dong

Li-zhi

et al. 2019

Genes

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“…However, how these signals regulate gene expression in the nucleus is largely unknown. The identification of wound-induced AP2/ERFs suggests the early wound signal rapidly reprograms the transcriptome at the transcriptional level (Marhava et al, 2019;Zhang et al, 2019;Zhou et al, 2019). Intriguingly, studies from the Sugimoto lab have shown that another group of AP2/ERF-type transcriptional factor genes, named WOUND-INDUCED DEDIFFERENTIATION1 (WIND1) and its homologs WIND2, WIND3 and WIND4, is induced upon wounding (Iwase et al, 2011a;2011b).…”

Section: Nature Of the Wound Signal During Plant Regenerationmentioning

confidence: 99%

“…Free IAA content measurement in Figures 2D, 2E, and 5K and Supplemental Figure 9H was performed as described in Wang et al (2015a), with minor modifications in ultraperformance liquid chromatographytandem mass spectrometer conditions. IAA content measurement in Figure 2F and Supplemental Figure 5D was performed as described by Zhang et al (2019). Briefly, the leaf explants from each sample were ground by liquid nitrogen, dissolved by 200 mL of phosphate buffered saline for 10 min on ice, and centrifuged at 12,000 rpm at 4°C for 10 min.…”

Section: Free Iaa Analysesmentioning

confidence: 99%

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

et al. 2019

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Age and wounding are two major determinants for regeneration. In plants, the root regeneration is triggered by woundinduced auxin biosynthesis. As plants age, the root regenerative capacity gradually decreases. How wounding leads to the auxin burst and how age and wound signals collaboratively regulate root regenerative capacity are poorly understood. Here, we show that the increased levels of three closely-related miR156-targeted Arabidopsis (Arabidopsis thaliana) SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors, SPL2, SPL10, and SPL11, suppress root regeneration with age by inhibiting wound-induced auxin biosynthesis. Mechanistically, we find that a subset of APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factors including ABSCISIC ACID REPRESSOR1 and ERF109 is rapidly induced by wounding and serves as a proxy for wound signal to induce auxin biosynthesis. In older plants, SPL2/10/11 directly bind to the promoters of AP2/ERFs and attenuates their induction, thereby dampening auxin accumulation at the wound. Our results thus identify AP2/ERFs as a hub for integration of age and wound signal for root regeneration.

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“…YUCCA1/4-mediated auxin biosynthesis promotes the fate transition of rooting competent cells in leaf explants of Arabidopsis (Chen et al, 2016). A wound-triggered signaling cascade mediated by jasmonate (JA) signaling was recently identified to stimulate auxin biogenesis for adventitious rooting (Zhang et al, 2019). JA-induced ETHYLENE RESPONSE FACTOR 109 directly activates the transcription of ANTHRANILATE SYNTHASE α1 encoding a tryptophan biosynthetic enzyme for auxin production (Zhang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…A wound-triggered signaling cascade mediated by jasmonate (JA) signaling was recently identified to stimulate auxin biogenesis for adventitious rooting (Zhang et al, 2019). JA-induced ETHYLENE RESPONSE FACTOR 109 directly activates the transcription of ANTHRANILATE SYNTHASE α1 encoding a tryptophan biosynthetic enzyme for auxin production (Zhang et al, 2019). Increased auxin polar transport labeled by radioactive IAA was detected around the position of excision where ARs develop, in line with AR deficient phenotypes resulting from impaired polar auxin transport (Sukumar et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

ThemicroRNA476a/RFL module regulates adventitious root formation through a mitochondria-dependent pathway inPopulus

Luo

Tao

et al. 2019

Preprint

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21 Adventitious root (AR) formation at the base of stem cuttings determines the efficiency 22 of clonal propagation for woody plants. Many endogenous and environmental factors 23 influence AR formation. However, our knowledge about the regulation of AR 24 development by mitochondrial metabolism in plants is very limited. Here we identified 25 Populus-specific miR476a as a novel regulator of wound-induced adventitious rooting 26 via orchestrating mitochondrial homeostasis in poplar. MiR476a exhibited inducible 27 expression during AR formation and directly targets several Restorer of Fertility like 28 (RFL) genes encoding mitochondrion-localized pentatricopeptide repeat proteins. 29 Genetic modification of miR476-RFL expression revealed the miR476/RFL-mediated 30 dynamic regulation of mitochondrial homeostasis on AR formation in transgenic 31 poplar. Furthermore, mitochondrial perturbation via exogenous chemical inhibitor 32 validated that the miR476a/RFL-directed AR formation depended on mitochondrial 33 regulation though modulating the auxin pathway. Our results established a 34 miRNA-directed mitochondrion-auxin signaling cascade required for AR development, 35 providing novel insights into the understanding of mitochondrial regulation on plant 36 developmental plasticity. 37 38

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Jasmonate-mediated wound signalling promotes plant regeneration

Cited by 204 publications

References 44 publications

Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.

Identification of Shoot Differentiation-Related Genes in Populus euphratica Oliv.

AP2/ERF Transcription Factors Integrate Age and Wound Signals for Root Regeneration

ThemicroRNA476a/RFL module regulates adventitious root formation through a mitochondria-dependent pathway inPopulus

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