Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in ‘Zaosu’ Pear (Pyrus bretschneideri Rehd.) Fruit

Wu, Ting; Liu, Han-Ting; Zhao, Guangping; Song, Junxing; Wang, Xiaoli; Yang, Chengquan; Zhai, Rui; Wang, Zhigang; Ma, Fengwang; Xu, Lingfei

doi:10.3390/biom10020278

Cited by 29 publications

(18 citation statements)

References 67 publications

(76 reference statements)

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“…Our previous study indicated that lanolin promotes pear anthocyanin accumulation (Wu et al 2020). By further analyzing the transcriptomic data, we found that the expression levels of 61 WRKY TFs were upregulated after lanolin treatment (Tables S1 and S3).…”

Section: Pbwrky75 May Be Related To Pear Anthocyanin Synthesismentioning

confidence: 76%

PbWRKY75 promotes anthocyanin synthesis by activating PbDFR, PbUFGT, and PbMYB10b in pear

Cong

Sha

et al. 2021

Physiologia Plantarum

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Anthocyanins are common secondary metabolites in plants that impart red coloration to fruits and flowers. The important WRKY transcription factor family plays multifaceted roles in plant growth and development. In this study, we found a WRKY family gene, Pyrus bretschneideri WRKY75, that may be involved in anthocyanin synthesis in pear. Unlike Arabidopsis thaliana WRKY75, PbWRKY75 may be a positive regulator of anthocyanin synthesis. A transient expression assay indicated that PbWRKY75 promoted pear anthocyanin synthesis. The structural genes (PbANS, PbDFR, and PbUFGT) and positive regulators (PbMYB10 and PbMYB10b) of anthocyanin synthesis were significantly upregulated in the fruitlet skins of PbWRKY75-overexpressing "Zaosu" pears. Subsequently, yeast one-hybrid and dual-luciferase assays indicated that PbWRKY75 promoted PbDFR, PbUFGT, and PbMYB10b expression by activating their promoters. These results revealed that PbWRKY75 may promote the expression of both PbMYB10b and anthocyanin late biosynthetic genes (PbDFR and PbUFGT) by activating their promoters, thereby inducing anthocyanin synthesis in pear. This study enhanced our understanding of the mechanism of pear anthocyanin synthesis, which will be beneficial in the improvement of pear peel color.

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Section: Pbwrky75 May Be Related To Pear Anthocyanin Synthesismentioning

confidence: 76%

PbWRKY75 promotes anthocyanin synthesis by activating PbDFR, PbUFGT, and PbMYB10b in pear

Cong

Sha

et al. 2021

Physiologia Plantarum

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“…In fruit, some studies focused on the ERF genes related with fruit colour, texture, flavor and ripening by using transgenosis or RNAi mostly existing in model fruit Tomato LeERF1 was found to be associated with fruit ripening and softening (Li et al, 2007) and Le-ERF2 could trigger fruit ethylene synthesis (Zhang et al, 2009). PbERF22 in pears was found to respond to jasmonate, ethylene signals and affect fruit anthocyanin biosynthesis (Wu et al, 2020). ERF genes have not been widely studied in different fruit species for the lack of genomic information.…”

Section: Discussionmentioning

confidence: 99%

The regulation of 1-methylcyclopropene treatment on the subfamily genes expression of ethylene response factors in peaches during storage

Cai

Han²,

Wang

et al. 2021

Acta Sci Pol Technol Aliment

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Background. Ethylene response factors (ERFs) perform diverse functions in fruit development, ripening and senescence. However, the effects of postharvest treatments on ERF genes have not been widely investigated due to the lack of peach ERF genomic information. The aim of this study was to investigate the ERF genes' expression of freshly harvested peach during storage after 1-methylcyclopropene (1-MCP) treatment. Material and methods. 10 μL L -1 1-MCP was used to fumigate peaches. Treated peaches and control peaches were stored at 20°C for 9 days. Fruit firmness, ethylene production and the transcript abundance of ERFs were evaluated during storage. Results. 127 AP2/ERF genes were identified genome using RNA-sequencing (RNA-seq). Expression profiles of 39 ERF genes were considered at day 0, 3, 5 and 7. Results showed that 1-MCP inhibited some ERF genes' expression (e.g., Prupe.5G117800), some genes were generally up-regulated responding to 1-MCP (e.g., Prupe.6G039700), while the other ERF genes displayed no significant difference between the two groups (e.g., Prupe.1G130300). Conclusions. These data revealed that peach ERF genes perform diverse functions during fruit growth, ripening and senescence. The different responses of ERF genes to postharvest 1-MCP treatment may be useful to understand the roles of ethylene and ERF genes in controlling technological aspects of postharvest peach conservation.

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“…Phytohormones are indispensable for the processes of plant growth and development, and known plant hormones, such as JA (jasmonate), ABA (abscisic acid), ethylene and auxin, could stimulate the ethylene response factor family (ERF) and be involved in the regulation of anthocyanin biosynthesis [55][56][57]. In red pear and A. thaliana, JAZ (jasmonate ZIM-domain) proteins are substrates of the SCF COI1 complex, which interferes with the transcriptional levels of WD/bHLH/MYB complexes by interacting directly with the C-terminus of both bHLH and MYB members, in turn repressing anthocyanin biosynthesis [58].…”

Section: Phytohormonesmentioning

confidence: 99%

Regulatory Mechanisms of Anthocyanin Biosynthesis in Apple and Pear

Liu

et al. 2021

IJMS

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Anthocyanins contribute to the quality and flavour of fruits. They are produced through the phenylpropanoid pathway, which is regulated by specific key genes that have been identified in many species. The dominant anthocyanin forms are reversibly transformed at different pH states, thus forming different colours in aqueous solutions. In plants, anthocyanins are controlled by specific factors of the biosynthetic pathway: light, temperature, phytohormones and transcription factors. Although great progress in research on anthocyanin structures and the regulation of anthocyanin biosynthesis has been made, the molecular regulatory mechanisms of anthocyanin biosynthesis in different plants remain less clear. In addition, the co-regulation of anthocyanin biosynthesis is poorly understood. In this review, we summarise previous findings on anthocyanin biosynthesis, including the biochemical and biological features of anthocyanins; differences in anthocyanin biosynthesis among fruit species, i.e., apple, red pear, and the model plant Arabidopsis thaliana; and the developmental and environmental regulation of anthocyanin accumulation. This review reveals the molecular mechanisms underlying anthocyanin biosynthesis in different plant species and provides valuable information for the development of anthocyanin-rich red-skinned and red-fleshed apple and pear varieties.

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Jasmonate and Ethylene-Regulated Ethylene Response Factor 22 Promotes Lanolin-Induced Anthocyanin Biosynthesis in ‘Zaosu’ Pear (Pyrus bretschneideri Rehd.) Fruit

Cited by 29 publications

References 67 publications

PbWRKY75 promotes anthocyanin synthesis by activating PbDFR, PbUFGT, and PbMYB10b in pear

PbWRKY75 promotes anthocyanin synthesis by activating PbDFR, PbUFGT, and PbMYB10b in pear

The regulation of 1-methylcyclopropene treatment on the subfamily genes expression of ethylene response factors in peaches during storage

Regulatory Mechanisms of Anthocyanin Biosynthesis in Apple and Pear

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