Japanese Encephalitis Virus (JEV) NS1′ Enhances the Viral Infection of Dendritic Cells (DCs) and Macrophages in Pig Tonsils

Xie, Shengda; Pan, Junhui; Zhang, Qiuting; Guan, Ruting; Yang, Xingmiao; Yang, Xiaoxiao; Liang, Zhenjie; Cao, Rui

doi:10.1128/spectrum.01147-22

Cited by 7 publications

(2 citation statements)

References 49 publications

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“…PRF is utilized by many viruses to increase the coding capacity within a limited viral genome, and the related translation product always benefits virus infection of the host. For example, NS1’, a PRF product of Japanese encephalitis virus, enhanced the viral infection of dendritic cells and macrophages [ 31 ], while the live-attenuated vaccine SA 14 -14–2 strain, which had lost NS1’ expression, showed slower replication kinetics in cells and was essentially non-neuroinvasive and non-neurovirulent in weanling ICR mice [ 32 ]. A transcription factor protein produced by alphaviruses via a -1 PRF played a role in viral assembly, as transcription factor-deficient virus exhibited reduced virion release [ 33 ] and attenuated pathogenesis [ 34 – 36 ].…”

Section: Discussionmentioning

confidence: 99%

Cell fusing agent virus isolated from Aag2 cells does not vertically transmit in Aedes aegypti via artificial infection

Zhou,

Huang,

Guo

et al. 2023

Parasites Vectors

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Background Cell fusing agent virus (CFAV) was the first insect-specific virus to be characterized, and has been reported to negatively influence the growth of arboviruses such as dengue, Zika, and La Cross, making it a promising biocontrol agent for mosquito-borne disease prevention. Aedes aegypti Aag2 cells were naturally infected with CFAV. However, the ability of this virus to stably colonize an Ae. aegypti population via artificial infection and how it influences the vector competence of this mosquito have yet to be demonstrated. Methods CFAV used in this study was harvested from Aag2 cells and its complete genome sequence was obtained by polymerase chain reaction and rapid amplification of complementary DNA ends, followed by Sanger sequencing. Phylogenetic analysis of newly identified CFAV sequences and other sequences retrieved from GenBank was performed. CFAV stock was inoculated into Ae. aegypti by intrathoracic injection, the survival of parental mosquitoes was monitored and CFAV copies in the whole bodies, ovaries, and carcasses of the injected F0 generation and in the whole bodies of the F1 generation on different days were examined by reverse transcription-quantitative polymerase chain reaction. Results The virus harvested from Aag2 cells comprised a mixture of three CFAV strains. All genome sequences of CFAV derived from Aag2 cells clustered into one clade but were far from those isolated or identified from Ae. aegypti. Aag2-derived CFAV efficiently replicated in the mosquito body and did not attenuate the survival of Ae. aegypti. However, the viral load in the ovarian tissues was much lower than that in other tissues and the virus could not passage to the offspring by vertical transmission. Conclusions The results of this study demonstrate that Aag2-derived CFAV was not vertically transmitted in Ae. aegypti and provide valuable information on the colonization of mosquitoes by this virus. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Cell fusing agent virus isolated from Aag2 cells does not vertically transmit in Aedes aegypti via artificial infection

Zhou,

Huang,

Guo

et al. 2023

Parasites Vectors

View full text Add to dashboard Cite

show abstract

“…It is generally believed that NS1’ is produced by programmed ribosomal frameshiftthat causes 52 amino acids added to the C-terminus of NS1. NS1′ is commonly associated with flavivirus replication complexes and plays a role in viral neuroinvasiveness ( Melian et al., 2010 ; Xie et al., 2022 ; Young et al., 2013 ). In addition, multiple reports have showed that JEV NS1′ is involved in regulating the host antiviral response ( Li et al., 2021 ).…”

Section: Flavivirus Ns1: a Multifunctional Proteinmentioning

confidence: 99%

Making sense of flavivirus non-strctural protein 1 in innate immune evasion and inducing tissue-specific damage

Zeng,

Liu,

Hao

et al. 2023

Virus Research

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Evaluation of the genotype I Japanese encephalitis virus as a stable viral vector for foreign gene expression

Zhang

Chen

et al. 2023

Antiviral Research

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Japanese Encephalitis Virus (JEV) NS1′ Enhances the Viral Infection of Dendritic Cells (DCs) and Macrophages in Pig Tonsils

Abstract: Pigs are amplification hosts for Japanese encephalitis virus (JEV). JEV can persist in the tonsils for months despite the presence of neutralizing antibodies.

Cited by 7 publications

References 49 publications

Cell fusing agent virus isolated from Aag2 cells does not vertically transmit in Aedes aegypti via artificial infection

Cell fusing agent virus isolated from Aag2 cells does not vertically transmit in Aedes aegypti via artificial infection

Making sense of flavivirus non-strctural protein 1 in innate immune evasion and inducing tissue-specific damage

Evaluation of the genotype I Japanese encephalitis virus as a stable viral vector for foreign gene expression

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